高效液相色谱法测定生地黄和桃红四物汤中梓醇的含量

doi:10.3736/jcim20040218

Abstract

Objective: To determinate the catalpol contents in dried rehmannia root and Taohong Siwu Decoction containing rehmannia root with high performance liquid chromatography (HPLC). Methods: Catalpol was separated on a YWG-C18 column using water-acetonitrile (99.4∶0.6) as mobile phase and detective wavelength at 210 nm. Results: The linear curve of tested catalpol concentration within the range of 0.0536-5.3600 μg/μl was ideal (n=5, r=0.9997). The average recovery rate of the dried rehmannia root and Taohong Siwu decoction was 98.7% (RSD=0.48%) and 98.2% (RSD=1.29%) respectively. Conclusion: HPLC method is accurate and valuable for the quality control of Radix Rehmanniae and Taohong Siwu Decoction.

Key words: Radix Rehmanniae, Taohong Siwu Decoction, catalpol, high performance liquid chromatography

CLC Number:

R286.0

Chun-yan Guo, Xue-mei Bai, Wan-ming Zhang, Zhi-bao Wang, Yu-rong Fu. Determination of catalpol in dried rehmannia root and Taohong Siwu Decoction with high performance liquid chromatography. Journal of Chinese Integrative Medicine, 2004, 2(2): 135-137.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20040218

http://www.jcimjournal.com/EN/Y2004/V2/I2/135

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	夏伦祝 . HPLC测定生地黄浓缩颗粒中梓醇的含量[J].安徽中医学院学报, 1999,18(4):51-52
[3]	欧明 . 汉英常用中医处方手册(第1版)[M]. 广州: 广东科学技术出版社, 1991,( 第1版):208

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Group	Added （mg）	Detected （mg）	Recovery (%)	?x (n=5, %)	RSD (n=5, %)
Dried rehmannia root
	0.4	0.392 9	98.23
	0.4	0.396 9	99.22
	0.3	0.295 2	98.40
	0.3	0.297 5	99.17
	0.2	0.197 0	98.50
				98.70	0.48
Taohong Siwu Decoction
	0.4	0.400 6	100.15
	0.4	0.393 9	98.48
	0.3	0.290 5	96.83
	0.3	0.294 3	98.10
	0.2	0.194 8	97.40
				98.20	1.29

Sample	Dried rehmannia root			Taohong Siwu Decoction
Sample	Catapol concentration（%）	?x(n=5, %)	RSD (n=5, %)	Catapol concentration（%）	?x(n=5, %)	RSD (n=5, %)
1	1.19			1.71
2	1.15			1.74
3	1.12			1.78
4	1.18			1.62
5	1.21			1.73
		1.17	3.02		1.72*	3.45

Determination of catalpol in dried rehmannia root and Taohong Siwu Decoction with high performance liquid chromatography

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