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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 132-135.doi: 10.3736/jcim20050214

• Original Experimental Research • Previous Articles     Next Articles

Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats

Bin Zhang1,Mo-bin Wan1,Ling-tai Wang2   

  1. 1. Department of Infectious Diseases, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Department of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Received:2004-09-20 Online:2005-03-20 Published:2005-03-20
  • Contact: Bin Zhang E-mail:zhxy5278@hotmail.com

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Objective

To study the therapeutic effect of Bushen Rougan Recipe (BSRGR) on hepatic fibrosis in rats.

Methods

Forty male Wistar rats were randomly divided into normal control group (n=10), model group (n=15), and BSRGR-treated group (n=15). Rats in the model and BSRGR-treated groups were administered intraperitoneally with 0.5% dimethylnitrosamine (DMN),10 ml·kg -1·d -1 , successive 3 days per week for 4 weeks to induce hepatic fibrosis. Then rats in the above 2 groups were given normal saline and BSRGR (10 ml·kg -1·d -1, ig) for another 4 weeks, respectively. Rats in the 3 groups were all executed at the end of the 8th week. The serum total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (A) and globulin (G) were determined and the serum hyaluronic acid (HA), laminin (LN) and collagen Ⅳ (Ⅳ-C) were measured. 

Results

The rat model of liver fibrosis was successfully induced by DMN. It was found that the serum TBIL, AST and ALT and the liver fibrosis marks were declined in BSRGR-treated group as compared with those in the model group (P<0.01). The content of total serum protein and the A/G in BSRGR-treated group were both increased as compared with those in the model group (P<0.05).

Conclusion

BSRGR can be used to treat hepatic fibrosis in rats.

Key words: Bushen Rougan Recipe, hepatic fibrosis, dimethylnitrosamine, animals, laboratory

CLC Number: 

  • R285.5

Tab 1

Comparison of serum TBIL, ALT and AST levels in 3 groups ($\bar{x}$±s)"

Group n TBIL (μmol/L) ALT (U/L) AST (U/L)
Normal control 10 1.53±0.22 30.50±4.74 128.30±7.06
Model 9 37.44±2.13** 107.33±14.62** 308.89±34.97**
BSRGR-treated 12 17.79±3.04 △△ 68.92±7.05 △△ 203.33±19.09 △△

Tab 2

Comparison of serum TP, A, G and A/G levels in 3 groups ($\bar{x}$±s)"

Group n TP (g/L) A (g/L) G (g/L) A/G
Normal control 10 75.50±4.60 40.30±3.23 35.20±2.62 1.15±0.11
Model 9 59.33±6.87** 22.67±3.04** 36.67±4.53** 0.62±0.07**
BSRGR-treated 12 69.83±3.71△△ 29.79±1.80△△ 40.04±2.34△△ 0.77±0.04△

Tab 3

Comparison of serum HA, LN and Ⅳ-C levels in 3 groups ($\bar{x}$±s)"

Group n HA (μg/L) LN (μg/L) Ⅳ-C (μg/L)
Normal control 10 252.90±26.59 37.90±5.97 21.40±4.17
Model 9 434.44±98.81** 70.67±6.32** 79.39±10.52**
BSRGR-treated 12 319.75±63.23 △△ 44.83±4.09△△ 52.79±5.71△△
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