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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (2): 132-135.doi: 10.3736/jcim20050214

• Original Experimental Research • Previous Articles     Next Articles

Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats

Bin Zhang1,Mo-bin Wan1,Ling-tai Wang2   

  1. 1. Department of Infectious Diseases, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    2. Department of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
  • Received:2004-09-20 Online:2005-03-20 Published:2005-03-20
  • Contact: Bin Zhang


To study the therapeutic effect of Bushen Rougan Recipe (BSRGR) on hepatic fibrosis in rats.


Forty male Wistar rats were randomly divided into normal control group (n=10), model group (n=15), and BSRGR-treated group (n=15). Rats in the model and BSRGR-treated groups were administered intraperitoneally with 0.5% dimethylnitrosamine (DMN),10 ml·kg -1·d -1 , successive 3 days per week for 4 weeks to induce hepatic fibrosis. Then rats in the above 2 groups were given normal saline and BSRGR (10 ml·kg -1·d -1, ig) for another 4 weeks, respectively. Rats in the 3 groups were all executed at the end of the 8th week. The serum total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (A) and globulin (G) were determined and the serum hyaluronic acid (HA), laminin (LN) and collagen Ⅳ (Ⅳ-C) were measured. 


The rat model of liver fibrosis was successfully induced by DMN. It was found that the serum TBIL, AST and ALT and the liver fibrosis marks were declined in BSRGR-treated group as compared with those in the model group (P<0.01). The content of total serum protein and the A/G in BSRGR-treated group were both increased as compared with those in the model group (P<0.05).


BSRGR can be used to treat hepatic fibrosis in rats.

Key words: Bushen Rougan Recipe, hepatic fibrosis, dimethylnitrosamine, animals, laboratory

CLC Number: 

  • R285.5

Tab 1

Comparison of serum TBIL, ALT and AST levels in 3 groups ($\bar{x}$±s)"

Group n TBIL (μmol/L) ALT (U/L) AST (U/L)
Normal control 10 1.53±0.22 30.50±4.74 128.30±7.06
Model 9 37.44±2.13** 107.33±14.62** 308.89±34.97**
BSRGR-treated 12 17.79±3.04 △△ 68.92±7.05 △△ 203.33±19.09 △△

Tab 2

Comparison of serum TP, A, G and A/G levels in 3 groups ($\bar{x}$±s)"

Group n TP (g/L) A (g/L) G (g/L) A/G
Normal control 10 75.50±4.60 40.30±3.23 35.20±2.62 1.15±0.11
Model 9 59.33±6.87** 22.67±3.04** 36.67±4.53** 0.62±0.07**
BSRGR-treated 12 69.83±3.71△△ 29.79±1.80△△ 40.04±2.34△△ 0.77±0.04

Tab 3

Comparison of serum HA, LN and Ⅳ-C levels in 3 groups ($\bar{x}$±s)"

Group n HA (μg/L) LN (μg/L) Ⅳ-C (μg/L)
Normal control 10 252.90±26.59 37.90±5.97 21.40±4.17
Model 9 434.44±98.81** 70.67±6.32** 79.39±10.52**
BSRGR-treated 12 319.75±63.23 △△ 44.83±4.09△△ 52.79±5.71△△
[1] Friedman SL . Molecular mechanisms of hepatic fibrosis and principles of therapy[J]. J Gastroenterol, 1997,32(3):424-430
doi: 10.1007/BF02934504 pmid: 9213261
[2] Lamireau T, Desmouliere A, Bioulac-Sage P , et al. Mechanisms of hepatic fibrogenesis[J]. Arch Pediatr, 2002,9(4):392-405
doi: 10.1016/S0929-693X(01)00800-4
[3] Housset C, Guechot J . Hepatic fibrosis: physiopathology and biological diagnosis[J]. Pathol Biol (Paris), 1999,47(9):886-894
pmid: 10609268
[4] Albanis E, Friedman SL . Hepatic fibrosis. Pathogenesis and principles of therapy[J]. Clin Liver Dis, 2001,5(2):315-334
doi: 10.1016/S1089-3261(05)70168-9
[5] Levy MT , McCaughan GW, Marinos G, et al.Intrahepatic expression of the hepatic stellate cell marker fibroblast activation protein correlates with the degree of fibrosis in hepatitis C virus infection[J]. Liver, 2002,22(2):93-101
doi: 10.1034/j.1600-0676.2002.01503.x
[6] Liu P, Liu CH, Wang HN , et al. Effect of salvianolic acid B on collagen production and mitogen-activated protein kinase activity in rat hepatic stellate cells[J]. Acta Pharmacol Sin, 2002,23(8):733-738
[7] 周飞, 张斌, 王灵台 , 等. 抗纤复方对硬变肝原代培养肝细胞超微结构的影响[J]. 辽宁中医杂志, 2001,28(11):699-700
[8] 包云, 张斌, 王灵台 . 抗纤复方对纤维肝大鼠肝细胞基底膜基质生成的影响[J]. 现代中西医结合杂志, 2001,10(17):1620-1622
[9] 高月求, 王灵台, 陈建杰 , 等. 补肾冲剂治疗慢性乙型肝炎的临床研究[J]. 上海中医药大学学报, 2001,15(1):34-36
doi: 10.3969/j.issn.1008-861X.2001.01.012
[10] 张斌, 万谟彬, 王灵台 . 肝纤维化大鼠TGF-β1与TIMP-1 mRNA的表达及补肾柔肝方的治疗作用[J]. 中西医结合学报, 2004,2(4):274-277
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