补肾柔肝方治疗大鼠肝纤维化的实验研究

doi:10.3736/jcim20050214

Abstract

Objective

To study the therapeutic effect of Bushen Rougan Recipe (BSRGR) on hepatic fibrosis in rats.

Methods

Forty male Wistar rats were randomly divided into normal control group (n=10), model group (n=15), and BSRGR-treated group (n=15). Rats in the model and BSRGR-treated groups were administered intraperitoneally with 0.5% dimethylnitrosamine (DMN),10 ml·kg ^-1·d ^-1 , successive 3 days per week for 4 weeks to induce hepatic fibrosis. Then rats in the above 2 groups were given normal saline and BSRGR (10 ml·kg ^-1·d ^-1, ig) for another 4 weeks, respectively. Rats in the 3 groups were all executed at the end of the 8th week. The serum total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (A) and globulin (G) were determined and the serum hyaluronic acid (HA), laminin (LN) and collagen Ⅳ (Ⅳ-C) were measured.

Results

The rat model of liver fibrosis was successfully induced by DMN. It was found that the serum TBIL, AST and ALT and the liver fibrosis marks were declined in BSRGR-treated group as compared with those in the model group (P<0.01). The content of total serum protein and the A/G in BSRGR-treated group were both increased as compared with those in the model group (P<0.05).

Conclusion

BSRGR can be used to treat hepatic fibrosis in rats.

Key words: Bushen Rougan Recipe, hepatic fibrosis, dimethylnitrosamine, animals, laboratory

CLC Number:

R285.5

Bin Zhang, Mo-bin Wan, Ling-tai Wang. Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats. Journal of Chinese Integrative Medicine, 2005, 3(2): 132-135.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050214

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References 10

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Group	n	TBIL (μmol/L)	ALT (U/L)	AST (U/L)
Normal control	10	1.53±0.22	30.50±4.74	128.30±7.06
Model	9	37.44±2.13**	107.33±14.62**	308.89±34.97**
BSRGR-treated	12	17.79±3.04 ^△△	68.92±7.05 ^△△	203.33±19.09 ^△△

Group	n	TP (g/L)	A (g/L)	G (g/L)	A/G
Normal control	10	75.50±4.60	40.30±3.23	35.20±2.62	1.15±0.11
Model	9	59.33±6.87**	22.67±3.04**	36.67±4.53**	0.62±0.07**
BSRGR-treated	12	69.83±3.71^△△	29.79±1.80^△△	40.04±2.34^△△	0.77±0.04^△

Group	n	HA (μg/L)	LN (μg/L)	Ⅳ-C (μg/L)
Normal control	10	252.90±26.59	37.90±5.97	21.40±4.17
Model	9	434.44±98.81**	70.67±6.32**	79.39±10.52**
BSRGR-treated	12	319.75±63.23 ^△△	44.83±4.09^△△	52.79±5.71^△△

Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats

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