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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 216-219.doi: 10.3736/jcim20050314

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shengji Huayu Recipe and its decomposed formulas on synthesis of collagen types Ⅰ and Ⅲ in granulation tissue of rats in early wound healing

Bin Li,Zhen-yi Wang,Xiu-li Xiao,Fu-lun Li,Bin Fan   

  1. Department of Chinese Traditional Surgery, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2005-03-23 Online:2005-05-20 Published:2005-05-20
  • Contact: Bin Li E-mail:drlibin@hotmail.com

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Objective

To study the effects of Shengji Huayu Recipe (a traditional Chinese medicine compound recipe for resolving stagnation and promoting granulation) and its decomposed formulas (Huayu Recipe for resolving stagnation and Shengji Recipe for promoting granulation) on the synthesis of collagen types Ⅰ and Ⅲ in granulation tissue of rats in early wound healing.

Methods

Twenty-four male Sprague-Dawley (SD) rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu Recipe-treated group, Shengji Recipe-treated group, Huayu Recipe-treated group and untreated group. Collagen types Ⅰ and Ⅲ in granulation tissue of the rats were tested with immunohistochemical methods and image analysis.

Results

On the third day of wound healing, collagen Ⅰ of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group, and collagen Ⅰ of the rats in Huayu Recipe-treated group was lower than that in the untreated group (P<0.05). Collagen Ⅲ of the rats in the three treated groups were lower than that in the untreated group (P<0.05). On the seventh day of wound healing, Collagen Ⅰ of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group (P<0.05), and collagen Ⅲ of the rats in both Shengji Recipe-treated group and Huayu Recipe-treated group was higher than that in the untreated group (P<0.05).

Conclusion

Resolving stagnation and promoting granulation therapy can promote the wound healing in rats.

Key words: Shengji Huayu Recipe, Wound healing, Granulation tissue, Collagen type Ⅰ, Collagen type Ⅲ

CLC Number: 

  • R283.61

Tab 1

Collagen types Ⅰ and Ⅲ in granulation tissue of the rats in 4 groups 3 days after skin lesion ($\bar{x}$±s, ％)"

Group n Collagen Ⅰ Collagen Ⅲ
Untreated group 6 39.0±4.7 54.1±7.8
Shengji Recipe-treated group 6 63.3±3.8* 34.0±5.5*
Huayu Recipe-treated group 6 25.9±4.2* 16.8±3.5*
Shengji Huayu Recipe-treated group 6 69.0±1.9* 41.8±6.4*

Fig 1

Collagen Ⅰ in granulation tissue of the rats in 4 groups 3 days after skin lesion (SP×40) A: Untreated group; B: Shengji Recipe-treated group; C: Huayu Recipe-treated group; D: Shengji Huayu Recipe-treated group"

Fig 2

Collagen Ⅲ in granulation tissue of the rats in 4 groups 3 days after skin lesion (SP×40) A: Untreated group; B: Shengji Recipe-treated group; C: Huayu Recipe-treated group; D: Shengji Huayu Recipe-treated group"

Tab 2

Collagen types Ⅰ and Ⅲ in granulation tissue of the rats in 4 groups 7 days after skin lesion ($\bar{x}$±s, ％)"

Group n Collagen Ⅰ Collagen Ⅲ
Untreated group 6 14.8±2.5 39.3±9.8
Shengji Recipe-treated group 6 6.0±1.4* 61.4±4.0*
Huayu Recipe-treated group 6 13.2±5.0 56.9±4.1*
Shengji Huayu Recipe-treated group 6 11.0±2.7* 37.9±5.1

Fig 3

Collagen Ⅰ in granulation tissue of the rats in 4 groups 7 days after skin lesion (SP×40) A: Untreated group; B: Shengji Recipe-treated group; C: Huayu Recipe-treated group; D: Shengji Huayu Recipe-treated group"

Fig 4

Collagen Ⅲ in granulation tissue of the rats in 4 groups 7 days after skin lesion (SP×40) A: Untreated group; B: Shengji Recipe-treated group; C: Huayu Recipe-treated group; D: Shengji Huayu Recipe-treated group"

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