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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 624-627.doi: 10.3736/jcim20060617

• Original Experimental Research • Previous Articles     Next Articles

Comparative study of the effects of Gypsum Fibrosum and Gypsum Fibrosum Preparatum in promoting granulation

Xiang Li1,Yuan-fen Liu1,Xiao-ren Xiang2,Yi-biao Zhou1,Zhi-lei Zhang1   

  1. 1. Department of Medicinal Chemistry, College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
    2. Key Laboratory of Acupuncture of Jiangsu Province,Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Online:2006-11-30 Published:2006-11-15

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Objective

To investigate the effects of Gypsum Fibrosum and Gypsum Fibrosum Preparatum in promoting granulation.

Methods

The wounds of muscle layer were produced in rats by using surgical operation. Two round wounds, with diameter about 1.5 cm, were cut at the depilatory area of two sides of the back of each rat, with an interval of 2 cm, deep to muscle layer, and the thickness of the knife wound of muscle layer was about 0.15 cm. Forty SD rats with the wounds were randomly divided into 4 groups: untreated group, beifuji-treated group, Gypsum Fibrosum-treated group and Gypsum Fibrosum Preparatum-treated group, with 10 rats in each group. Then the wounds were sprinkled with powders of Gypsum Fibrosum and Gypsum Fibrosum Preparatum, or sprayed with beifuji solution, respectively. The healing state of granulation tissues of the wounds was observed at the eighth and fourteenth day respectively.

Results

The number of fibroblasts, the number of capillary tubes and the area of capillary tubes in granulation tissue of wounds in the Gypsum Fibrosum Preparatum-treated group were significantly higher than those in the untreated group and Gypsum Fibrosum-treated group (P＜0.01). There were no statistical differences between the Gypsum Fibrosum Preparatum-treated group and the beifuji-treated group. However, Gypsum Fibrosum-treated group showed no obvious differences compared to the untreated group (P＞0.05). 

Conclusions

Gypsum Fibrosum Preparatum can accelerate the formation of collagenoblast and micrangium in wounds, and the proliferation of granulation tissues, thus promoting the skin wounds to healing. The effect of Gypsum Fibrosum is changed after being calcined, and Gypsum Fibrosum Preparatum has obvious effect in promoting granulation.

Key words: Gypsum Fibrosum, Gypsum Fibrosum Preparatum, Trauma, granulation tissue, Promoting granulation

CLC Number: 

  • R685

Figure 1

Pathological changes after 8-day administration in 4 groups (HE staining, ×1200)A: Untreated group, fibroblasts dispersed over large numbers of inflammatory cells; B: Beifuji-treated group, granulation tissue with more new capillary tubes and more regenerative cells; C: Gypsum Fibrosum Preparatum-treated group, granulation tissue with more new capillary tubes and more regenerative cells; D: Gypsum Fibrosum-treated group, granulation tissue with less new capillary tubes and the fibroblasts dispersed over some inflammatory cells."

Figure 2

Pathological changes after 14-day administration in 4 groups (HE staining, ×1200) A: Untreated group, granulation tissue with less new capillary tubes; B: Beifuji-treated group, granulation tissue with more new capillary tubes and gradual fibrosis; C: Gypsum Fibrosum Preparatum-treated group, granulation tissue with more new capillary tubes and gradual fibrosis; D: Gypsum Fibrosum-treated group, granulation tissue with slight fibrosis."

Table 1

Number of fibroblasts, capillary tubes and total area of capillary tubes in granulation tissue of wound of rats in 4 groups (ヌ±S)"

GroupnNumber of
fibroblasts		Number of
capillary tubes		Total area of
capillary tubes (μm2)
Untreated
8th day1033.5±5.26.3±0.91 848±605
14th day1034.1±1.28.2±1.23 001±1 106
Beifuji-treated
8th day1045.3±3.5**10.5±2.0**6 288±1 812**
14th day1053.4±4.4**12.5±1.1**7 380±1 133**
Gypsum Fibrosum-treated
8th day1030.1±1.96.7±1.23 417±2 122*
14th day1031.2±3.28.0±0.72 724±765
Gypsum Fibrosum Preparatum-treated
8th day1053.5±6.9**△△9.3±1.7**△△6 446±2 292**△△
14th day1054.1±4.7**△△11.3±1.2**△△7 729±1 966**△△

Table 2

Surface density, number density and perimeter density of capillary tubes in granulation tissue of wound of rats in 4 groups (ヌ±S)"

GroupnSurface density
of capillary tubes
(μm2/μm2)		Number density
of capillary tube
(×10–5/μm2)		Perimeter density
of capillary tubes
(×10–4μm/μm2)
Untreated
8th day100.017±0.0065.7±0.837.3±8.3
14th day100.028±0.0107.6±1.150.9±12.4
Beifuji-treated
8th day100.058±0.017**9.7±1.8**89.6±15.2**
14th day100.068±0.001**11.5±1.0**98.4±8.9**
Gypsum Fibrosum-treated
8th day100.031±0.020*6.1±1.149.5±16.6
14th day100.024±0.0077.4±0.748.2±7.5
Gypsum Fibrosum Preparatum-treated
8th day100.059±0.021**△△8.5±1.6**△△75.5±19.9**△△
14th day100.071±0.018**△△10.4±1.1**△△98.6±13.6**△△
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