Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 220-223.doi: 10.3736/jcim20050315

• Original Experimental Research • Previous Articles     Next Articles

Effects of Wenshen Jianpi Recipe on chronic wound healing in rats

Yong-qing Cao,Chun-mei He,Jin-gen Lu   

  1. Shanghai Municipal Clinical Medicine Center for Chinese Traditional Surgery, Shanghai 200032, China
  • Received:2005-03-29 Online:2005-05-20 Published:2005-05-20
  • Contact: Yong-qing Cao


To study the effects of Wenshen Jianpi Recipe (WSJPR, a traditional Chinese medicine for warming kidney and invigorating spleen) on chronic wound healing and the mechanism.


Ninety-six SD rats were randomly divided into 4 groups, with 24 rats in each group, and back wound was made in the rats. For rats in 3 of the 4 groups, hydrocortisone injection was administered to induce chronic wound. Rats in 2 of the 3 groups were treated with WSJPR and Xinpukang Granules (XPKG) respectively, and the rats in the other group were untreated. The rats in the fourth group were taken as control. The wound healing time and the width of new epidermis were observed, and the histomorphological changes and cell cycle of the granulation tissue, and the protein expressions of epidermal growth factor (EGF), transforming growth factor-β1 (TGF-β1) and fibronectin (FN) in the granulation tissue were tested with immunohistochemical technique and flow cytometry.


The wound healing time of the WSJPR-treated and XPKG-treated groups was (17.0±1.9) and (18.8±1.9) d respectively, much shorter than that of the untreated and control groups (P<0.05). On the 14th experiment day, the width of new epidermis of the WSJPR-treated and XPKG-treated groups was (3.73±0.19) and (3.21±0.15) mm respectively, much wider than that of the untreated and control groups (P<0.05). The numbers of angiogenesis, fibroblasts and cells in the S phase in WSJPR-treated and XPKG-treated groups were much higher than those in the untreated and control groups (P<0.05). Compared with the untreated and control groups, the protein expressions of EGF, TGF-β1 and FN in WSJPR-treated and XPKG-treated groups were higher (P<0.05).


WSJPR can enhance the wound healing. It was likely through accelerating the cell proliferation and up-regulating the expressions of EGF, TGF-β1 and FN.

Key words: Pharmacotherapy, TCM, Wound healing, Granulation tissue, Histology, Comparative, Cytokines

CLC Number: 

  • R283.661

Tab 1

Wound healing time and width of new epidermis on the 14th experiment day ($\bar{x}$±s)"

Group n Wound healing time (d) Width of new epidermis (mm)
Untreated group 8 22.4±2.1 1.96±0.14
Control group 8 20.1±1.8 2.64±0.21
WSJPR-treated group 8 17.0±1.9* 3.73±0.19*
XPKG-treated group 8 18.8±1.9* 3.21±0.15*

Tab 2

Scores of histomorphological examination of granulation tissue($\bar{x}$±s)"

Group n Angiogenesis Fibroblast Necrosis
Untreated group 6 1.50±0.55 1.33±0.52 1.83±0.75
Control group 6 1.67±0.82 1.67±0.82 1.67±0.52
WSJPR-treated group 6 2.33±0.52*△▲ 2.50±0.55*△▲ 1.16±0.75
XPKG-treated group 6 1.83±0.75 1.83±0.75 1.50±0.55

Tab 3

Cells in different cell cycles of granulation tissue($\bar{x}$±s, %)"

Group n G0-G1 G2-M S
Untreated group 8 84.9±2.6 3.6±1.1 11.5±1.9
Control group 8 82.6±1.7 3.8±0.8 13.6±2.1
WSJPR-treated group 8 82.1±2.8 3.5±0.5 14.6±2.9*△▲
XPKG-treated group 8 82.6±2.1 3.2±1.1 14.2±1.2

Tab 4

Protein expressions of EGF, TGF-β1 and FN in granulation tissue ($\bar{x}$±s)"

Group n IOD (×103)
Untreated group 4 61.28±8.95 62.26±2.85 121.03±35.69
Control group 4 86.42±11.70 268.53±107.20 284.00±66.88
WSJPR-treated group 4 179.47±35.56* 452.64±56.32* 482.57±16.91*
XPKG-treated group 4 106.08±28.09* 281.33±20.04 339.96±60.19*
[1] 傅小兵, 王亚平, 常国友 , 等. 碱性成纤维细胞生长因子促进受创皮肤再生的实验研究[J]. 中国修复重建外科杂志, 1996,10(1):23-25
[2] 陈奇 . 中药药理实验方法学(第1版)[M]. 北京: 人民卫生出版社, 1994,( 第1版):176
[3] 傅小兵, 王德文 . 创伤修复基础(第1版)[M]. 北京: 人民军医出版社, 1997,( 第1版):182
[4] Brown KJ, Maynes SF, Bezos A , et al. A novel in vitro assay for human angiogenesis[J]. Lab Invest, 1996,75(4):539-555
pmid: 8874385
[5] 陈奇 . 中药药理研究方法学(第1版)[M]. 北京: 人民卫生出版社, 1993,( 第1版):982
[6] 陈星伟 . 纤维结合蛋白在创伤愈合中的作用[J]. 中华创伤杂志, 1990,6(4):245-247
[1] Gerli E.G. Herr, Fábio Goulart da Silva, Francisco José Cidral-Filho, Fabricia Petronilho, Lucinéia Gainski Danielski, Mariana Pereira de Souza Goldim, Afonso Shiguemi Inoue Salgado, Franciane Bobinski, Daniel Fernandes Martins, Eliane R. Winkelmann. Effects of the use of bioceramic wraps in patients with lower limb venous ulcers: A randomized double-blind placebo-controlled trial. Journal of Integrative Medicine, 2020, 18(1): 27-.
[2] Pádraic J.Dunne, Julie Lynch, Lucia Prihodova, Caoimhe O'Leary, Atiyeh Ghoreyshi, Sharee A. Basdeo, Donal J.Cox, Rachel Breen, Ali Sheikhi, Áine Carroll, Cathal Walsh, Geraldine McMahon, Barry White. Burnout in the emergency department: Randomized controlled trial of an attention-based training program. Journal of Integrative Medicine, 2019, 17(3): 173-180.
[3] J.M. Oliver-Baxter, H.S. Whitford, D.A. Turnbull, M.J. Bond. Effects of vitamin supplementation on inflammatory markers and psychological wellbeing among distressed women: A randomized controlled trial. Journal of Integrative Medicine, 2018, 16(5): 322-328.
[4] Uday P. Pratap, Hannah P. Priyanka, Karthik R. Ramanathan, Vishak Raman, Lalgi Hima, Srinivasan Thyagarajan. Noni (Morinda citrifolia L.) fruit juice delays immunosenescence in the lymphocytes in lymph nodes of old F344 rats. Journal of Integrative Medicine, 2018, 16(3): 199-207.
[5] Fabiana de Almeida Andrade, Caio Fabio Schlechta Portella. Research methods in complementary and alternative medicine: An integrative review. Journal of Integrative Medicine, 2018, 16(1): 6-13.
[6] Lilian Uchoa Carneiro, Ilna Gomes da Silva, Mirian Eliza Alves de Souza, Wellington da Silva Côrtes, Mário Geraldo de Carvalho, Bruno Guimarães Marinho. Antinociceptive and anti-inflammatory activities of leaf extracts from Annona tomentosa R.E.Fr. Journal of Integrative Medicine, 2017, 15(5): 379-387.
[7] Qing Ji, Yun-quan Luo, Wen-hai Wang, Xuan Liu, Qi Li, Shi-bing Su. Research advances in traditional Chinese medicine syndromes in cancer patients. Journal of Integrative Medicine, 2016, 14(1): 12-21.
[8] Cláudia Maria Sousa, Luis Moreira, Daniela Coimbra, Jorge Machado, Henry J. Greten. Immediate effects of Tuina techniques on working-related musculoskeletal disorder of professional orchestra musicians. Journal of Integrative Medicine, 2015, 13(4): 257-261.
[9] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[10] Bing He, Ge Zhang, Ai-ping Lu. Integrative network analysis: Bridging the gap between Western medicine and traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(3): 133-135.
[11] Zhao-guo Li, Xi-ru Liu. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases of Paediatrics (Part 3). Journal of Integrative Medicine, 2015, 13(3): 201-204.
[12] Xiao Ye, Jing Wang. On English translation of infant Tuina points in traditional Chinese medicine. Journal of Integrative Medicine, 2015, 13(3): 205-207.
[13] Zhao-guo Li. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases of Paediatrics (Part 2). Journal of Integrative Medicine, 2015, 13(2): 129-132.
[14] Zhao-guo Li . Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases of Paediatrics (Part 1). Journal of Integrative Medicine, 2015, 13(1): 61-64.
[15] Arthur de Sá Ferreira, Antonio Guilherme Pacheco. SimTCM: A human patient simulator with application to diagnostic accuracy studies of Chinese medicine. Journal of Integrative Medicine, 2015, 13(1): 9-19.
Full text



[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[3] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[4] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[5] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[6] Ya-bing Zhou, Jian-er Yu, Jie Wu, Li Bai, Li-li Huo, Xin-guang Zhang, Li-qing Li . Effects of Chinese herbal medicine Bushen Gubiao Recipe on toll-like receptor 4 and CD4 +CD25 +foxp3 +regulatory T cells in mice with recurrent respiratory tract infections. Journal of Chinese Integrative Medicine, 2010, 8(11): 1053-1059
[7] Chan Chen, Yong-mei Meng , Peng Zhang, Juan Wang, Hui-hui Zhao, Shu-zhen Guo , Wei Wang. Diagnosis and treatment rule of traditional Chinese medicine for syndrome factors of chronic congestive heart failure: a study based on Shannon entropy method. Journal of Chinese Integrative Medicine, 2010, 8(11): 1080-1084
[8] Nian-hong Wang , Jun-tao Yan , Wu-quan Sun , Yong-shan Hu , Jun Xia , Li-cheng Wei , Jie Jia , Gui-lin Ouyang , Yong He , Yan-ming Guo , Jie Xu . Effects of early application of Tuina treatment on quadriceps surface myoelectricity in patients after total knee arthroplasty: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1247-1253
[9] Yuan-jie Wu , Zhao-hui Fang , Shu-guo Zheng , Yuan-bo Wu , Ai-hua Fan. Effects of Chinese herbal medicine Danzhi Jiangtang Capsule and exercise on JNK signaling pathway in pancreatic tissues of diabetic rats. Journal of Chinese Integrative Medicine, 2012, 10(11): 1279-1285
[10] Li-ke Guo, Chao-xian Zhang, Xiao-feng Guo. Acupuncture combined with Chinese herbal medicine Plantain and Senna Granule in treatment of functional constipation: A randomized, controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(11): 1206-1214