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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 247-250.doi: 10.3736/jcim20060305

• Original Clinical Research • Previous Articles     Next Articles

Effects of Yisui Shengxue Granules on expressions of alpha-hemoglobin stabilizing protein and erythroid transcription factor GATA-1 mRNAs in bone marrow of patients with β-thalassemia

Yong-mei Liu1, Zhi-kui Wu1, Li-min Chai2, Xin-hua Zhang3   

  1. 1. Department of Molecular Biology, Guang’anmen Hospital, China Academy of Traditional Chinese Medicine, Beijing 100053, China;
    2. Hospital of Ophthal
  • Online:2006-05-31 Published:2006-05-15

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Objective

To evaluate the effects of Yisui Shengxue Granules on expressions of alpha-hemoglobin stabilizing protein (AHSP) and erythroid transcription factor GATA-1 mRNAs in bone marrow of patients with β-thalassemia, and to explore its possible molecular mechanism.

Methods

Twelve patients with β-thalassemia intermedia were treated with Yisui Shengxue Granules for three months. The blood indexes including hemoglobin (Hb), RBC, fetal hemoglobin (HbF) and reticulated corpuscles (Ret) were examined before and after treatment. Total RNA was extracted from bone marrow karyocyte in 8 patients selected from these 12 patients before and after treatment, and the expression levels of the AHSP and GATA-1 mRNAs were measured by real-time PCR.

Results

Yisui Shengxue Granules could not only obviously improve the clinical symptoms of patients with β-thalassemia intermedia, but also obviously increased the contents of Hb, RBC, HbF and Ret (P＜0.05, or P<0.01). The expression levels of AHSP and GATA-1 mRNAs also significantly increased after treatment as compared with those before treatment (P＜0.05, or P<0.01). 

Conclusion

The results revealed that one of the possible molecular mechanism of the effects caused by Yisui Shengxue Granules is that it can up-regulate the expression levels of AHSP and erythroid transcription factor GATA-1 mRNAs, enhance the protein synthesis of AHSP which can bind the relative excess free alpha-globin, prevent the formation of alpha -globin-cytotoxic precipitates in red blood cells and decrease the hemolysis.

Key words: Yisui Shengxue Granules, β-thalassemia, α-hemoglobin stabilizing protein, GATA-1, Genes

CLC Number: 

  • R556.7

Table 1

Changes of blood indexes in patients with β-thalassemia before and after treatment with Yisui Shengxue Granules（ヌ±S ）"

TimenHb (g/L)RBC (×1012/L)HbF (%)Ret (%)
Before treatment1272.167±13.0443.626±0.7950.167±0.1300.051±0.018
After treatment1281.917±13.938**4.103±0.745**0.195±0.145*0.081±0.029**

Table 2

Expression levels of AHSP and GATA-1 mRNAs in bone marrow karyocyte of patients with β-thalassemia before and after treatment with Yisui Shengxue Granules （ヌ±S ）"

Timen Ct ratio of AHSP/β-actinCt ratio of GATA-1/β-actin
Before treatment81.230±0.0601.183±0.086
After treatment81.092±0.142*1.100±0.094**

Figure 1

Electrophoregram of PCR products of β-actinA-H: PCR products of β-actin (263 bp); I: DL2000 marker."

Figure 2

Electrophoregram of PCR products of AHSPA-F: PCR products of AHSP (190 bp); G-H: PCR products of β-actin (263 bp); I: DL2000 marker."

Figure 3

Electrophoregram of PCR products of GATA-1A-F: PCR products of GATA-1 (130 bp); G: DL2000 marker."

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