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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (9): 870-876.doi: 10.3736/jcim20100911

• Original Experimental Research • Previous Articles     Next Articles

Tea polyphenol inhibits colorectal cancer with microsatellite instability by regulating the expressions of HES1, JAG1, MT2A and MAFA

YY Xu,HY Jin,XZ Tan,XF Liu,YJ Ding   

  1. National Center of Colorectal Surgery, The 3rd Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210001, Jiangsu Province, China
  • Received:2010-03-05 Accepted:2010-06-18 Online:2010-09-20 Published:2010-09-15


To investigate the mechanism of tea polyphenol in inhibiting microsatellite instability (MSI) of colorectal cancer.

Using LoVo cells and SW480 cells treated with aqueous solution of tea polyphenol, cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) method, changes in microsatellite sequences were detected by genescan method and changes in gene expression of LoVo cells were detected by illumina expression arrays and quantitative real-time polymerase chain reaction (PCR).

The proliferation inhibition rates of LoVo and SW480 cells treated with tea polyphenol increased with the increasing of drug concentration and showed an increasing tendency with time. The proliferation inhibition rate of LoVo cells with tea polyphenol was higher than that of SW480 cells, and there was a significant difference in the proliferation inhibition rates at 24 h, 72 h and one week. The microsatellite sequence of LoVo cells treated with tea polyphenol remained stable. The gene expression arrays and quantitative real-time PCR suggested that tea polyphenol inhibited the gene expressions of MT2A, MAFA, HES1 and JAG1 nearly two-fold over controls. It was also found that tea polyphenol inhibited the BAX and p38 genes with a more than two-fold difference but did not significantly inhibit the nuclear factor-κB pathway.

Tea polyphenol significantly inhibited the proliferation of MSI colorectal cancer cells and stably maintained the microsatellite state in MSI colorectal cancer. Tea polyphenol inhibited the gene expressions of HES1, JAG1, MT2A and MAFA, up-regulated the gene expression of BAX and down-regulated that of P38. Further research is required to investigate how these pathways are interrelated.

Key words: Tea polyphenols, Colorectal cancer, Microsatellite instability, Gene expression

Table 1

Forward and reverse primers used for real-time fluorescent quantitative PCR"

Figure 2

Inhibition rate on LoVo cells of tea polyphenol at different times"

Figure 2

Inhibition rate on SW480 cells of tea polyphenol at different times"

Table 2

Length of miscrosatellite marker after treatment with tea polyphenol"

Table 3

Up-regulated and down-regulated genes tested by expression microarray in LoVo cells after treatment with tea polyphenol"

Table 4

Expressions of mRNAs detected by real-time fluorescent quantitative PCR in LoVo cells"

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