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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 271-274.doi: 10.3736/jcim20060310

• Original Experimental Research • Previous Articles     Next Articles

Effects of Fuzheng Yiliu Granules on apoptotic rate and mitochondrial membrane potential of hepatocellular carcinoma cell line H22 from mice

Jian-xiong Zhao, Fu-lian Guo, De-cheng Bai, Xue-xi Wang   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Lanzhou University, Lanzhou, Gansu Province 730000, China
  • Online:2006-05-31 Published:2006-05-15

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Objective

To evaluate the effects of Fuzheng Yiliu Granules (FZYLG) on apoptotic rate and mitochondrial membrane potential (Δψm) of hepatocellular carcinoma cell line H22 from mice.

Methods

Forty-eight mice inoculated with H22 cells were randomly divided into four groups: untreated group, cyclophosphamide-treated group, high-dose FZYLG-treated group and low-dose FZYLG-treated group. After 14 days of corresponding treatment, H22 cells in each group were stained with propidium iodide, and the apoptotic rates were detected by flow cytometry (FCM). The rhodamine 123 was used as a fluorescence probe to label the H22 cells, and the fluorescence intensities were observed with laser scanning confocal microscope. The fluorescence intensity of H22 cells indicated the Δψm of H22 cells.

Results

FZYLG could significantly increase the apoptotic rate while reduce the Δψm of H22 cells from mice as compared with those in the untreated group.

Conclusion

The antitumor effects of FZYLR on H22 cells from mice are related to decreasing the Δψm and then inducing the apoptotsis of the H22 cells.

Key words: Fuzheng Yiliu Granules, Carcinoma, Hepatocellular, Apoptosis, Mitochondrial membrane potential

CLC Number: 

  • R246.6

Table 1

Effects of FZYLG on apoptotic rates of H22 cells from mice（ヌ±S, %）"

Group n Apoptotic rate
Untreated 12 27.02±0.83
Cyclophosphamide-treated 12 71.45±1.34**
High-dose FZYLG-treated 12 45.33±1.63**
Low-dose FZYLG-treated 12 37.69±1.11*

Table 2

Effects of FZYLG on mitochondrial membrane potentials (Δψm) of H22 cells from mice（ヌ±S）"

Group n Average fluorescence intensity of Rhodamine 123
Untreated 12 230.13±57.46
Cyclophosphamide-treated 12 106.20±27.60**
High-dose FZYLG-treated 12 124.44±68.35**
Low-dose FZYLG-treated 12 207.97±64.70*

Figure 1

Fluorescence intensities of Rhodamine 123 in H22 cells from mice in different groups (by laser scanning confocal microscope)A: Untreated group; B: Cyclophosphamide-treated group; C: High-dose FZYLG-treated group; D: Low-dose FZYLG-treated group."

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