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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (3): 271-274.doi: 10.3736/jcim20060310

• Original Experimental Research • Previous Articles     Next Articles

Effects of Fuzheng Yiliu Granules on apoptotic rate and mitochondrial membrane potential of hepatocellular carcinoma cell line H22 from mice

Jian-xiong Zhao, Fu-lian Guo, De-cheng Bai, Xue-xi Wang   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Lanzhou University, Lanzhou, Gansu Province 730000, China
  • Online:2006-05-31 Published:2006-05-15


To evaluate the effects of Fuzheng Yiliu Granules (FZYLG) on apoptotic rate and mitochondrial membrane potential (Δψm) of hepatocellular carcinoma cell line H22 from mice.


Forty-eight mice inoculated with H22 cells were randomly divided into four groups: untreated group, cyclophosphamide-treated group, high-dose FZYLG-treated group and low-dose FZYLG-treated group. After 14 days of corresponding treatment, H22 cells in each group were stained with propidium iodide, and the apoptotic rates were detected by flow cytometry (FCM). The rhodamine 123 was used as a fluorescence probe to label the H22 cells, and the fluorescence intensities were observed with laser scanning confocal microscope. The fluorescence intensity of H22 cells indicated the Δψm of H22 cells.


FZYLG could significantly increase the apoptotic rate while reduce the Δψm of H22 cells from mice as compared with those in the untreated group.


The antitumor effects of FZYLR on H22 cells from mice are related to decreasing the Δψm and then inducing the apoptotsis of the H22 cells.

Key words: Fuzheng Yiliu Granules, Carcinoma, Hepatocellular, Apoptosis, Mitochondrial membrane potential

CLC Number: 

  • R246.6

Table 1

Effects of FZYLG on apoptotic rates of H22 cells from mice(ヌ±S, %)"

Group n Apoptotic rate
Untreated 12 27.02±0.83
Cyclophosphamide-treated 12 71.45±1.34**
High-dose FZYLG-treated 12 45.33±1.63**
Low-dose FZYLG-treated 12 37.69±1.11*

Table 2

Effects of FZYLG on mitochondrial membrane potentials (Δψm) of H22 cells from mice(ヌ±S)"

Group n Average fluorescence intensity of Rhodamine 123
Untreated 12 230.13±57.46
Cyclophosphamide-treated 12 106.20±27.60**
High-dose FZYLG-treated 12 124.44±68.35**
Low-dose FZYLG-treated 12 207.97±64.70*

Figure 1

Fluorescence intensities of Rhodamine 123 in H22 cells from mice in different groups (by laser scanning confocal microscope)A: Untreated group; B: Cyclophosphamide-treated group; C: High-dose FZYLG-treated group; D: Low-dose FZYLG-treated group."

[1] 赵健雄, 程卫东, 徐瑞峰 , 等. 扶正抑瘤颗粒药物血清诱导小鼠肝癌细胞凋亡及其机制的研究[J]. 中西医结合学报, 2005,3(4):278-281
doi: 10.3321/j.issn:1003-5370.2003.12.008
[2] 赵健雄, 曲勇, 陈学忠 , 等. 扶正抑瘤颗粒对食管癌、胃癌组织细胞周期及核转录因子的影响[J]. 中国中西医结合杂志, 2003,23(12):908-910
doi: 10.7661/CJIM.2003.12.908
[3] 陈德昌, 祝晓光, 陈桂英 , 等. 中药消癌散抗肿瘤作用的实验观察[J]. 蚌埠医学院学报, 1999,24(3):149-150
doi: 10.3969/j.issn.1000-2200.1999.03.005
[4] 陈扬超, 周茂华, 周克元 , 等. 顺铂诱导鼻咽癌CNE-2Z细胞凋亡并降低其线粒体膜电位[J]. 癌症, 2001,20(8):887-888
doi: 10.3969/j.issn.1000-467X.2001.08.028
[5] 连平, 赵健雄, 沈世林 , 等. 扶正抑瘤颗粒对乳腺癌术后患者T细胞亚群NK细胞及全血粘度影响的临床研究[J]. 中华实用中西医杂志, 2003,3(16):431-432
[6] 张晓晖, 姚天明, 黄高昇 , 等. 细胞凋亡的最新研究进展[J]. 第四军医大学学报, 2002,23(增刊):42-44
[7] Green DR, Reed JC . Mitochondria and apoptosis[J]. Science, 1998,281(5381):1309-1312
[8] 彭心昭, 陈英, 乔东访 , 等. 长春新碱诱导的自噬性细胞凋亡对线粒体膜电位的影响[J]. 中国医学物理学杂志, 2001,18(1):37-39
doi: 10.3969/j.issn.1005-202X.2001.01.017
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