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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (8): 843-851.doi: 10.3736/jcim20080814

• Original Experimental Research • Previous Articles     Next Articles

Differentially expressed genes in adrenal gland of H22 liver cancer mice with different syndromes and in different stages

Zhi-qiang Pan1, Zhao-qin Fang1(), Wen-li Lu1, Chao Liang1, Zhong-hua Wu1, Xiao-mei Liu1, Li Hou1, Hui Zhang1, Shao-yuan Zhuo1, Ming-juan Liao1, Bi-feng Gao2   

  1. 1. School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Medicial College of Coloroda State University, Denver CO 80262, United States of America
  • Received:2008-01-14 Online:2008-08-20 Published:2008-08-15

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Objective: To reveal the characteristics of gene expression in adrenal gland of H22 tumor mice with typical syndromes and in different liver cancer stages.
Methods: By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed adrenal gland gene expression in H22 tumor mice with pathogenic factor-toxin predominance syndrome and qi deficiency syndrome in the earlier stage, yang-qi deficiency syndrome in the intermediate stage, and qi-yin-yang deficiency syndrome in the advanced stage. Genes highly expressed and remarkably different were analyzed in this study.
Results: A total of seventy-three up-regulated coincident genes and twenty-six down-regulated coincident genes in different stages were investigated in the study. Up-regulated coincident genes included Hp, C3, Anxa1, Procr, C2, Il4ra, Cd14, Ptprc, Cd52, C4b, Eno3, Xdh, Gpx3, and so on. Down-regulated coincident genes included nervous system function-related genes such as Plp1, Mbp, Aldh1a1, Cck, Atn1, genes associated with electrolyte metabolism such as Aldh1a1 and Slc22a17, genes related to signal transduction such as Cxcr4, Spag5 and Stmn3, etc, and genes related to transcriptional control and protein biosynthesis such as Hspa1a, Dnajb1, Thra, Hhex and so on.
Conclusion: With the development of the tumorigenesis, the symptoms and signs and differentially expressed genes in adrenal gland of H22 tumor mice can be measured. Up-regulated and down-regulated coincident genes may be the features of H22 tumor mice different from those of normal mice.

Key words: liver cancer, symptom complex, adrenal gland, neoplasm staging, gene expression, GeneChip

CLC Number: 

  • R735.7

Table 1

Up-regulated genes of mice with PTPS and QDS in earlier stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_011723 1 628 3 259 3 008 2 756 2 575 Xdh Chemical modification
NM_008161 1 414 3 004 2 286 2 526 2 491 Gpx3 Metabolism
NM_008149 1 287 2 991 2 567 2 565 2 870 Gpam Chemosynthesis, metabolism
NM_009621 737 2 222 1 622 1 406 1 355 Adamts1 Metabolism, signal transduction
NM_213660 1 059 2 124 1 652 2 065 2 115 Stat3 Regulation, JAK-STAT
NM_011171 893 2 015 1 696 1 776 1 703 Procr Immunization
NM_011498 574 2 012 1 160 1 091 1 273 Bhlhb2 Regulation
NM_013484 760 1 716 1 715 1 298 1 672 C2 Metabolism, immunization
NM_133857 640 1 709 1 106 1 175 1 040 Usp53 Metabolism
NM_011580 460 1 329 1 068 1 298 1 001 Thbs1 Growth and development
NM_008873 324 1 237 871 723 592 Plau Metabolism, signal transduction
NM_025967 563 1 213 941 1 192 974 D16Ertd472e
NM_007469 417 1 190 951 840 1 098 Apoc1 Transport, metabolism
NM_177317 0 1 166 940 468 2 Fpr-rs7
NM_001030305 393 1 139 1 032 853 885 Pmp2 Transport
XM_977523 364 1 117 579 723 639 Atxn7 Growth and development
NM_026268 1 325 2 312 2 709 2 524 2 630 Dusp6 Cell cycle, MAPK
NM_019472 548 1 216 1 288 945 875 Myo10 Signal transduction
NM_027209 573 921 1 261 926 895 Ms4a6b Signal transduction

Table 2

Up-regulated genes of mice with YQDS in intermediate stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_007933 1 655 3 774 2 880 4 060 4 023 Eno3 Chemolysis
NM_009626 1 513 2 745 2 782 3 432 3 152 Adh7 Regulation
NM_001008700 702 1 694 1 638 2 279 2 206 Il4ra Immunization
AK076887 910 1 436 1 848 1 960 1 568 Drctnnb1a
NM_173051 491 861 768 1 914 1 495 Serpinb1c
NM_009653 490 1 546 950 1 738 1 301 Alas2 Chemosynthesis
NM_008953 16 87 67 1 445 192 Psp
NM_008555 582 1 066 1 029 1 398 1 097 Masp1 Chemolysis, immunization
NM_181320 616 1 294 932 1 296 1 033 Dusp16 Chemical modification
NM_178098 91 267 265 1 267 968 4930486L24Rik
NM_009994 573 1 042 887 1 167 957 Cyp1b1 Immunization, chemical modification
NM_172964 304 648 597 1 010 894 Arhgap28

Table 3

Up-regulated genes of mice with QYYDS in advanced stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_009654 1 224 4 626 4 677 2 582 5 332 Alb1 Transport, signal transduction
NM_017370 2 299 3 768 3 574 3 961 4 602 Hp Chemolysis, immunization
NM_009114 37 300 227 2 422 4 245 S100a9 Regulation
NM_008491 93 362 281 2 525 4 242 Lcn2 Transport
NM_017371 120 1 615 1 124 2 287 4 165 Hpxn Metabolism,transport
NM_009778 1 056 2 044 1 690 2 376 3 009 C3 Immunization, signal transduction
NM_008694 16 121 94 1 266 2 925 Ngp
NM_139198 753 1 317 1 219 1 913 2 171 Plac8
NM_010196 134 1 261 866 965 2 052 Fga
NM_181849 76 1 088 744 913 1 988 Fgb
NM_029796 353 663 535 1 434 1 882 Lrg1
NM_011314 0 709 271 928 1 766 Saa2
NM_133862 95 1 034 776 737 1 673 Fgg Signal transduction, regulation
NM_013465 253 1 204 1 135 788 1 500 Ahsg Growth and development, signal transduction
XM_001002722 332 753 569 1 053 1 485 C4b Immunization
NM_010924 549 867 922 884 1 460 Nnmt
NM_009841 502 793 770 1 224 1 441 Cd14 Apoptosis, immunization
NM_010266 352 707 621 1 141 1 370 Gda Metabolism, growth and development
NM_018746 113 763 457 670 1 302 Itih4 Immunization
NM_023125 76 575 471 411 1 279 Kng1 Immunization
NM_011210 497 1 045 867 1 055 1 146 Ptprc Signal transduction,cell cycle
NM_008096 150 730 760 393 1 120 Gc Transport
NM_175933 554 1 028 1 081 957 1 115 Pex5 Transport
NM_010705 476 728 816 782 1 096 Lgals3
NM_009253 197 709 846 407 1 091 Serpina3m
NM_007376 93 674 566 363 1 087 Pzp
NM_145594 17 514 411 523 1 052 Fgl1
NM_028672 378 711 635 1 038 1 052 4930430E16Rik
NM_008625 523 800 806 1 033 1 049 Mrc1 Immunization
NM_011314 1 377 168 490 1 020 Saa2

Table 4

Gradually up-regulated genes of mice from earlier stage to advanced stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_013650 59 666 455 3 938 6 140 S100a8 Immunization
XM_993285 547 945 1 106 3 213 4 782 Igk-V21-9
NM_010730 932 1 413 1 600 2 486 3 652 Anxa1 Metabolism, cell cycle and apoptosis
NM_172768 1 652 3 082 3 297 3 425 3 491 Gramd1b
NM_009892 13 102 103 1 154 2 176 Chi3l3
NM_008522 28 106 106 1 014 2 173 Ltf Transport, immunization
NM_145122 902 1 496 1 554 1 799 2 119 Pex16 Growth and development
NM_013706 491 770 791 1 031 1 391 Cd52
NM_010824 10 77 100 616 1 205 Mpo Apoptosis, immunization
NM_008611 8 30 35 516 1 197 Mmp8 Metabolism, chemolysis
NM_001034870 327 644 754 992 1 184 Serpina3h
NM_011408 435 823 882 936 1 072 Slfn2

Table 5

Down-regulated genes of mice with PTPS and QDS in earlier stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_007906 1 270 415 461 831 809 Eef1a2 Chemosynthesis
NM_026159 2 029 588 1 289 1 033 620 Retsat Chemical modification,metabolism
NM_021551 1 599 727 1 009 944 1 055 Slc22a17 Transport
NM_177013 1 026 84 68 94 120 6332401O19Rik
NM_031161 1 933 166 155 224 180 Cck Signal transduction
NM_153581 1 143 274 272 349 315 Gpm6a
NM_007881 1 260 520 455 603 576 Atn1 Growth and development
NM_009133 1 178 565 495 693 610 Stmn3 Signal transduction,growth and development
NM_011428 1 781 676 593 974 857 Snap25 Signal transduction

Table 6

Down-regulated genes of mice with YQDS in intermediate stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_009508 1 342 89 97 58 91 Slc32a1 Transport
XM_990459 13992 154 132 127 6 690 Similar to H3
histone, family 3B
NM_001025255 4248 207 133 131 131 Mbp Immunization
NM_010479 2 798 482 271 156 232 Hspa1a chemical modification
NM_025491 1 145 460 302 249 257 Susd3
NM_017407 1 716 531 458 348 361 Spag5 Cell cycle, signal transduction
NM_025844 1 323 684 788 411 485 Chordc1
NM_172391 1 881 1 132 1 086 691 784 1110064P04Rik
NM_013559 3 188 1 799 1 418 713 831 Hsp110
NM_013467 3 346 1 259 1 394 913 922 Aldh1a1 Metabolism
NM_009911 3 022 1 863 1 206 1151 1 228 Cxcr4 Signal transduction

Table 7

Down-regulated genes of mice with QYYDS in advanced stage"

Genebank No. GeneChip reading Gene Function category
Normal PTPS QDS YQDS QYYDS
NM_013415 1 006 412 387 294 264 Atp1b2 Transport
NM_008245 1 071 514 572 387 362 Hhex Regulation
NM_011123 5 219 696 414 429 334 Plp1
NM_144921 1 367 624 618 505 411 Atp1a3 Transport
NM_178060 1 150 728 666 609 569 Thra Regulation
NM_018808 2 761 909 692 682 680 Dnajb1 Chemical modification
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