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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (10): 934-939.doi: 10.3736/jcim20091005

• Original Clinical Research • Previous Articles     Next Articles

Relationship between the phenotypes and functions of peripheral blood dendritic cells and the different spleen deficiency syndrome types in patients with chronic hepatitis B

 Lei Wanga, Xiao-xia Fengb,Wei Zhanga,Lian-jun Xingb,Pei-yong  Zhengb,Guang Jia   

  1. a Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b Department of Liver Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-06-25 Accepted:2009-08-12 Online:2009-10-20 Published:2009-10-15
  • Contact: Lian-jun Xing E-mail:jiliver@vip.sina.com

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Objective

To study the phenotypes and functions of dendritic cells (DCs) derived from peripheral blood monocytes of chronic hepatitis B (CHB) patients with different traditional Chinese medicine (TCM) syndrome types, and to explore the relationship between TCM syndrome type and DC functions.
Methods

Sixty CHB patients were included in this study. All the CHB patients were divided into spleen deficiency and liver stagnation, spleen deficiency and dampness-heat and deficiency of both spleen and kidney groups according to TCM syndrome diagnosis standard. There were 20 cases in each group, and ten healthy people were included as normal control. The volunteer’s peripheral blood was collected for monocyte separation, biochemical test and hepatitis B virus DNA loads detection. DCs were induced and isolated from peripheral blood monocytes, and then the expressions of surface markers CD80, CD86, CD1a and HLA-DR were detected by flow cytometric analysis method. Interleukin-10 (IL-10) production of the DCs was quantified by enzyme-linked immunosorbent assay.
Results

The proliferation of DCs in the CHB patients was slower than that in the healthy volunteers (P<0.05). The expressions of DC surface molecules such as CD80, CD86, and CD1a were obviously decreased in the CHB patients as compared with those in the healthy volunteers (P<0.05). More over, expressions of DC surface molecules were different among CHB patients with different TCM syndrome types. The positive expressions of CD80, CD1a, and HLA-DR in the CHB patients with spleen deficiency and liver stagnation were obviously higher than those in the CHB patients with deficiency of both spleen and kidney (P<0.05), and the CD1a expression in the CHB patients with spleen deficiency and dampness-heat was higher than that in the CHB patients with deficiency of both spleen and kidney (P<0.05). In DC culture supernatant, the IL-10 concentration of the CHB patients with deficiency of both spleen and kidney was higher than that of the CHB patients with spleen deficiency and liver stagnation (P<0.05), and the IL-10 concentrations of the CHB patients with different TCM syndrome types were higher than that of the healthy volunteers (P<0.05).
Conclusion

During the pathogenic course of CHB, the phenotypes and functions of DCs are different in CHB patients with different TCM syndrome types. It suggests that there is a correlation between TCM syndrome type and body immunity function.

Key words: Chronic hepatitis B, Dendritic cell, Traditional Chinese medicine, Spleen deficiency, Syndrome

Figure 1

Morphology of DC at different time points after adherent cultivation (Inverted phase contrast microscopy, ×200) A: Two hours after adherent cultivation; B: Day three after adherent cultivation; C: Day five after adherent cultivation; D: Day seven after adherent cultivation."

Figure 2

Morphology of mature DC at day seven after adherent cultivation (Electronmicroscopy, ×3 000)"

Table 1

Biochemistry and HBV-DNA loads of CHB patients with different TCM syndrome type"

Group n ALT
($\bar{x}$±s, U/L)		 AST
($\bar{x}$±s, U/L)		 TBIL
($\bar{x}$±s, μmol/L)		 HBV-DNA
[Median (Q25, Q75), IU/mL]
Spleen deficiency and liver stagnation 20 35.81±20.74 51.44±32.34 14.78±4.49 1.42×105 (6.04×104, 1.75×107)
Spleen deficiency and dampness-heat 20 61.06±66.75 65.14±47.33 16.54±5.85 4.19×106 (2.16×105, 1.52×107)
Deficiency of spleen and kidney 20 34.94±17.51 41.38±23.19 16.35±6.29 9.67×105 (9.77×104, 8.99×107)

Table 2

Expressions of DC surface markers in CHB patients with different TCM syndrome type ($\bar{x}$±s, %)"

Group n CD80 CD86 CD1a HLA-DR
Normal control 10 64.72±9.85 75.10±7.76 62.77±5.57 59.00±7.94
Spleen deficiency and liver stagnation 20 11.84±11.14* 54.51±12.14* 32.01±11.17* 51.42±12.09
Spleen deficiency and dampness-heat 20 9.00±7.55* 53.98±13.60* 29.30±11.28* 46.09±11.77*
Deficiency of spleen and kidney 20 5.94±2.62*△ 42.64±22.05* 22.14±10.37*△▲ 39.29±15.52*△

Figure 3

Expressions of DC surface markers in CHB patients with different TCM syndrome type A: Normal control group; B: Spleen deficiency and liver stagnation group; C: Spleen deficiency and dampness-heat group; D: Deficiency of spleen and kidney group."

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