[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 565-569.doi: 10.3736/jcim20110516

• Original Experimental Research • Previous Articles     Next Articles

Thread-dragging therapy in treatment of a rat model of infected subcutaneous fistula

Chen Wang1, Jin-gen Lu1, Hao-qiang Yin2, Yong-qing Cao1()   

  1. 1. Department of Anorectal Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Medical Imaging, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2011-01-15 Accepted:2011-02-21 Online:2011-05-20 Published:2011-05-15
  • Contact: Cao Yong-qing E-mail:caoyq88@sh163.net

Objective: This study was to develop an experimental rat model of subcutaneous fistula induced by bacteria infection and treat it with thread-dragging therapy.
Methods: A spring-gauze was surgically implanted into 24 male Sprague-Dawley rats’ dorsal muscles, respectively, then mixed Staphylococcus aureus and Escherichia coli were injected into the spring-gauze. After 45 d, all the spring-gauzes were removed from the rats under anesthesia. X-ray and ultrasound were performed to assess the fistula tract. Each rat was examined by a fistula probe and four rats were randomly selected for histological assessment. The residual rats were randomly divided into two groups. Group A was treated with thread-dragging therapy, and group B was treated with fistulotomy. The healing time and body weight of the two groups were compared.
Results: On the 45th day of the spring-gauze implantation, imaging assessment proved a tract with two openings in all rats and histological results proved that lumen and surrounding granulation tissue with epithelium were similar to the features of fistula in human beings. The mean healing time of rats in group A was shorter than that of the rats in group B (P<0.01). The body weight of rats in group A was heavier than that of the rats in group B on the 7th and 14th day after surgery, respectively (P<0.05).
Conclusion: A rat model of subcutaneous fistula induced by mixed Staphylococcus aureus and Escherichia coli infection was successfully established. Traditional Chinese medicine thread-dragging therapy is less invasive and safer than fistulotomy.

Key words: subcutaneous fistula, thread-dragging therapy, traditional Chinese medicine, models, animal

Figure 1

Spring-gauze was implanted beneath the rat’s dorsal muscle"

Figure 2

Thread-dragging therapy"

Figure 3

Fistulotomy therapy"

Figure 4

Little yellow pus can be found at the surface of spring-gauze after 45 d of modeling"

Figure 5

Histology of fistulas on the 45th day tested by hematoxylin and eosin staining (Light microscopy,×50) The arrows point the lumen of fistula track and the thickness of inner layer was uneven."

Figure 6

Two tiny scars on the rat’s skin with thread-dragging therapy"

Figure 7

Severe scar on the rat’s skin with fistulotomy"

"

[1] Wolff BG, Fleshman JW, Beck DE, Pemberton JH, Wexner SD . The ASCRS textbook of colon and rectal surgery[M]. New York: Springer, 2007: 198-214
[2] Buchanan GN, Sibbons P, Osborn M, Bartram CI, Ansari T, Halligan S, Cohen CR . Experimental model of fistula-in-ano[J]. Dis Colon Rectum, 2005,48(2):353-358
doi: 10.1007/s10350-004-0769-7 pmid: 15714247
[3] Zhao BM, Zhang SX. Study on diseases of large intestine and anus[M]. Shanghai: Second Military Medical University Press, 2004: 553
赵宝明, 张书信 . 大肠肛门病学[M]. 上海: 第二军医大学出版社, 2004: 553
[4] Lu JG, Que HF, Chen HF, Cao YQ, Wang YF, Wang C, Yao YB . Superiority of thread-dragging therapy in treatment of refractory sinus tracts or fistulae[J]. J Chin Integr Med, 2008,6(10):991-994
陆金根, 阙华发, 陈红风, 曹永清, 王云飞, 王琛, 姚一博 . 拖线疗法治疗难愈性窦瘘的优势[J]. 中西医结合学报, 2008,6(10):991-994
[5] Johnson EK, Gaw JU, Armstrong DN . Efficacy of anal fistula plug vs. fibrin glue in closure of anorectal fistulas[J]. Dis Colon Rectum, 2006,49(3):371-376
doi: 10.1007/s10350-005-0288-1 pmid: 16421664
[6] Lu JG, Cao YQ, He CM, Guo XT, Huang HX, Yi J, Xiao LX, Xu YM, Din M, Pan YB, Wang C . Clinical research of thread-dragging through fistula method in treating patients with simple anorectal fistula[J]. J Chin Integr Med, 2006,4(2):140-146
陆金根, 曹永清, 何春梅, 郭修田, 黄鸿翔, 易进, 肖立新, 徐昱旻, 丁敏, 潘一滨, 王琛 . 隧道式拖线术治疗单纯性肛瘘的临床研究[J]. 中西医结合学报, 2006,4(2):140-146
[7] Fitch MT, Manthey DE, McGinnis HD, Nicks BA, Pariyadath M .A skin abscess model for teaching incision and drainage procedures[J]. BMC Med Educ, 2008,8(1):38
doi: 10.1186/1472-6920-8-38 pmid: 2453116
[8] Grace RH, Harper IA, Thompson RG . Anorectal sepsis: microbiology in relation to fistula-in-ano[J]. Br J Surg, 1982,69(7):401-403
doi: 10.1002/bjs.1800690715 pmid: 7104611
[9] Lunniss PJ, Faris B, Rees HC, Heard S, Phillips RK . Histological and microbiological assessment of the role of microorganisms in chronic anal fistula[J]. Br J Surg, 1993,80(8):1072
doi: 10.1002/(ISSN)1365-2168
[10] Bao Y, Li YS, Li JS . A laboratory model of intraabdominal infections by intestinal fistula[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2004,21(1):99-100
doi: 10.3760/j.issn:1001-9030.2004.01.041
鲍扬, 李幼生, 黎介寿 . 一种新型腹腔感染动物模型的建立[J]. 中华实验外科杂志, 2004,21(1):99-100
doi: 10.3760/j.issn:1001-9030.2004.01.041
[11] Seow-Choen F, Hay AJ, Heard S, Phillips RK . Bacteriology of anal fistulae[J]. Br J Surg, 1992,79(1):27-28
doi: 10.1002/bjs.1800790107 pmid: 12587233
[12] Harding Rains AJ, Mann CV . Bailey and Love’s short practice of surgery[M]. London: H.K. Lewis, 1998,( 20th ed):116-117
[13] Lunniss PJ, Sheffield JP, Talbot IC, Thomson JP, Phillips RK . Persistence of idiopathic anal fistula may be related to epithelialization[J]. Br J Surg, 1995,82(1):32-33
doi: 10.1002/bjs.1800820112 pmid: 7881949
[14] Bataille F, Klebl F, Rümmele P, Schroeder J, Farkas S, Wild PJ, Fürst A, Hofstadter F, Scholmerich J, Herfarth H, Rogler G . Morphological characterisation of Crohn’s disease fistulae[J]. Gut, 2004,53(9):1314-1321
doi: 10.1136/gut.2003.038208 pmid: 1774207
[1] Zhao-guo Li , Rui-qiang Liu. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases in Internal Medicine (Part 5). Journal of Chinese Integrative Medicine, 2012, 10(9): 1061-1065.
[2] Zhao-lan Liu, Qing Li , Yu-jie Mu , Ying Gao, Jian-ping Liu. Using nested case-control study to appraise the effectiveness of Chinese medicines in the treatment of diabetic nephropathy. Journal of Chinese Integrative Medicine, 2012, 10(9): 991-996.
[3] Chen Xia , Qun-bang Zhu, Feng Huang, Jing-hui Huang, Hong-da Chen, Ming-jun Chen, Wei Gu, Bai Li, Fan-fu Fang, Liang-neng Wu . Traditional Chinese medicine constitution types in 127 elderly patients with insomnia: An investigation in communities of Yangpu District, Shanghai. Journal of Chinese Integrative Medicine, 2012, 10(8): 866-873.
[4] Claudia Maria Sous, Mario Goncalves, Jorge Machado, Thomas Efferth, Tobias Greten , Petra Froeschen , Henry J. Greten. Effects of qigong on performance-related anxiety and physiological stress functions in transverse flute music schoolchildren: A feasibility study. Journal of Chinese Integrative Medicine, 2012, 10(8): 858-865.
[5] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[6] Zhao-guo Li . Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases in Internal Medicine (Part 4). Journal of Chinese Integrative Medicine, 2012, 10(8): 948-952.
[7] Zhao-guo Li. Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases in Internal Medicine (Part 3). Journal of Chinese Integrative Medicine, 2012, 10(7): 827-831.
[8] Wei-hua Xu, Ze-huai Wen, Wei-xiong Liang, Qi Wang. A review of research on the development of instruments for therapeutic efficacy evaluation of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(7): 726-737.
[9] Ping Li, Yun-zhong Shi . A study on English translation of antithesis in Neijing. Journal of Chinese Integrative Medicine, 2012, 10(7): 832-836.
[10] Ming-xing Zhao, Zhen-hua Dong, Zhong-hai Yu, Shi-yuan Xiao, Ya-ming Li . Effects of Ginkgo biloba extract in improving episodic memory of patients with mild cognitive impairment: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(6): 628-634.
[11] Ying-fei Bi , Jing-yuan Mao , Xian-liang Wang , Ya-zhu Hou , Yi-zhu Lu , Shan Bin Soh , Bo-li Zhang. Clinical epidemiology survey of the traditional Chinese medicine etiology and syndrome differentiation of coronary artery disease: Study protocol of a multicenter trial. Journal of Chinese Integrative Medicine, 2012, 10(6): 619-627.
[12] Zhao-guo Li . Comparative study on WHO Western Pacific Region and World Federation of Chinese Medicine Societies international standard terminologies on traditional medicine: Diseases in Internal Medicine (Part 2). Journal of Chinese Integrative Medicine, 2012, 10(6): 712-716.
[13] C. Matos Luís, J. Goncalves Mário, R. Silva António, G. Mendes Joaquim, P. Machado Jorge, J. Greten Henry. Assessment of Qigong-related effects by infrared thermography: A case study. Journal of Chinese Integrative Medicine, 2012, 10(6): 663-666.
[14] Christoph A. Doenitz, Ana Anjos, Thomas Efferth , Tobias Greten, Henry J. Greten. Can heat and cold be parameterized? Clinical data of a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(5): 532-537.
[15] Zhao-guo Li . Issues on translation of terms in traditional Chinese medicine clinical practice. Journal of Chinese Integrative Medicine, 2012, 10(5): 591-596.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]