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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (9): 1005-1013.doi: 10.3736/jcim20110912

• Original Experimental Research • Previous Articles     Next Articles

Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis

Jong-Min Kim1, Kyung-Soon Kim1, Yeon-Weol Lee1, Chong-Kwan Cho1, Hwa-Seung Yoo1(), Ji-Young Bang2, Eung-Yoon Kim2, In-Cheol Kang2,3,4()   

  1. 1. East-West Cancer Center, Dunsan Oriental Medical Hospital of Daejeon University, Daejeon 302-122, Korea
    2. InnoPharmasrceen Inc., Hoseo University, Asan 336-795, Korea
    3. Department of Biological Science, College of Natural Science, Hoseo University, Asan 336-795, Korea
    4. BioChip Research Center, Hoseo University, Asan 336-795, Korea
  • Received:2011-03-23 Accepted:2011-04-25 Online:2011-09-20 Published:2011-09-15
  • Contact: Yoo Hwa-Seung,Kang In-Cheol E-mail:altyhs@dju.kr;ickang@hoseo.edu

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Objective: This study aimed to investigate the anti-angiogenic effects of the water extract of Pulsatilla koreana (Yabe ex Nakai) Nakai ex T. Mori., Panax ginseng C.A. Meyer and Glycyrrhiza uralensis Fisch (WEPPG).

Methods: The effects of WEPPG on fibroblast growth factor (bFGF)-induced angiogenesis were evaluated by human umbilical vein endothelial cell (HUVEC) proliferation, adhesion, and migration assays. Capillary tube formation of HUVECs and bFGF-induced chick chorioallantoic membrane (CAM) angiogenesis were also observed. WEPPG was used to treat the HUVECs and CAMs, and then various activities such as proliferation, adhesion, migration, capillary tube formation and cell cycle proteins were analyzed.

Results: WEPPG significantly inhibited bFGF-induced HUVEC proliferation, adhesion, migration, and capillary tube formation. Signaling protein analysis showed up-regulated expressions of various proteins including cyclin A, p63 and KIP2 and down-regulated expressions of nibrin and focal adhesion kinase. The blood vessel formation in a CAM treated with WEPPG was markedly reduced compared with the control group.

Conclusion: These results suggested that the inhibition of angiogenesis by WEPPG can be an action mechanism for its anti-cancer effects.

Key words: Pulsatilla , Panax ginseng, Glycyrrhiza uralensis, plant extracts, angiogenesis inhibitors, cyclin A, focal adhesion protein-tyrosine kinases, in vitro

Figure 1

Inhibitory effect of WEPPG on bFGF-induced angiogenesis: HUVEC proliferation"

Figure 2

Inhibitory effect of WEPPG on bFGF-induced angiogenesis: HUVEC attachment"

Figure 3

Inhibitory effect of WEPPG on bFGF-induced angiogenesis: HUVEC migration"

Figure 4

Inhibitory effects of WEPPG on capillary tube formation"

Figure 5

Inhibitory effects of WEPPG on bFGF-induced CAM angiogenesis"

"

Group n Number of anti-
angiogenic CAMs		 CAM angiogenesis
inhibition (%)
PBS control 10 0 0
bFGF control 10 1 10
GRGDSP control 10 9 90
WEPPG-treated 9 8 89
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