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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (6): 690-694.doi: 10.3736/jcim20120614

• Original Experimental Research • Previous Articles     Next Articles

Polyherbal formulation Bresol® protects the mast cells against compound 48/80-induced disruption and histamine release: A non-immunological mechanism of mast cell stabilization

Yathiraj Ashwini, Mohamed Rafiq, Gollapalle L. Viswanatha, Subbanna Rajesh, Selvam Senthilraja, Mohammed Azeemuddin(),Suryakanth,Rangesh Paramesh D. Anturlikar, Pralhad S. Patki#br#   

  1. Department of Pharmacology, Research and Development Center, the Himalaya Drug Company, Bangalore 562123, Karnataka, IndiaDepartment of Pharmacology, Research and Development Center, the Himalaya Drug Company, Bangalore 562123, Karnataka, India
  • Received:2012-01-06 Accepted:2012-03-13 Online:2012-06-20 Published:2018-06-15
  • Contact: Azeemuddin Mohammed

Objective: Present study was aimed to evaluate the protective effect of Bresol ?, a polyherbal formulation, on mast cell degranulation and histamine release from mast cells.
Methods: Mast cell-stabilizing activity of Bresol? was evaluated against compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells in ex vivo conditions.
Results: Microscopy of the control group smears showed more of intact mast cells, with very minimum number of degranulated mast cells and negligible amount of histamine release. In contrast, incubation of mast cells with compound 48/80 caused significant degranulation of the mast cells associated with release of high concentration of histamine in the positive control group. Furthermore, Bresol? at 100 mg/L showed a significant inhibition of compound 48/80-induced mast cell degranulation. In addition, Bresol? significantly and dose-dependently inhibited compound 48/80-induced histamine release.
Conclusion: Bresol? inhibits compound 48/80-induced mast cell degranulation and histamine release in ex vivo conditions. The present findings could be one of the non-immunological mechanism responsible for usefulness of Bresol? in various allergic conditions.

Key words: medicine, Ayurvedic, plants, medicinal, compound 48/80, mast cells, cell degranulation, histamine release, in vitro

Figure 1

The morphologies of the mast cells (Light microscopy, ×400) A: Type-Ⅰ cells (intact mast cells); B: Type-Ⅱ cells (partially degranulated mast cells); C: Type-Ⅲ cells (completely degranulated mast cells)."

Figure 2

Effect of Bresol? on compound 48/80-induced mast cell degranulation All the values are expressed as mean ± standard error of mean, n=6; *P<0.05, vs normal control group; △P<0.05, vs positive control group."

Figure 3

Standard curve of histamine The standard curve for histamine was obtained by plotting the fluorescence intensity of histamine-o-phthaladehyde adduct against the concentration of histamine (μg/L)."

Figure 4

Effect of Bresol? on compound 48/80-induced histamine release from mast cells"

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