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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (6): 690-694.doi: 10.3736/jcim20120614

• Original Experimental Research • Previous Articles     Next Articles

Polyherbal formulation Bresol® protects the mast cells against compound 48/80-induced disruption and histamine release: A non-immunological mechanism of mast cell stabilization

Yathiraj Ashwini, Mohamed Rafiq, Gollapalle L. Viswanatha, Subbanna Rajesh, Selvam Senthilraja, Mohammed Azeemuddin(),Suryakanth,Rangesh Paramesh D. Anturlikar, Pralhad S. Patki#br#   

  1. Department of Pharmacology, Research and Development Center, the Himalaya Drug Company, Bangalore 562123, Karnataka, IndiaDepartment of Pharmacology, Research and Development Center, the Himalaya Drug Company, Bangalore 562123, Karnataka, India
  • Received:2012-01-06 Accepted:2012-03-13 Online:2012-06-20 Published:2018-06-15
  • Contact: Azeemuddin Mohammed

Objective: Present study was aimed to evaluate the protective effect of Bresol ?, a polyherbal formulation, on mast cell degranulation and histamine release from mast cells.
Methods: Mast cell-stabilizing activity of Bresol? was evaluated against compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells in ex vivo conditions.
Results: Microscopy of the control group smears showed more of intact mast cells, with very minimum number of degranulated mast cells and negligible amount of histamine release. In contrast, incubation of mast cells with compound 48/80 caused significant degranulation of the mast cells associated with release of high concentration of histamine in the positive control group. Furthermore, Bresol? at 100 mg/L showed a significant inhibition of compound 48/80-induced mast cell degranulation. In addition, Bresol? significantly and dose-dependently inhibited compound 48/80-induced histamine release.
Conclusion: Bresol? inhibits compound 48/80-induced mast cell degranulation and histamine release in ex vivo conditions. The present findings could be one of the non-immunological mechanism responsible for usefulness of Bresol? in various allergic conditions.

Key words: medicine, Ayurvedic, plants, medicinal, compound 48/80, mast cells, cell degranulation, histamine release, in vitro

Figure 1

The morphologies of the mast cells (Light microscopy, ×400) A: Type-Ⅰ cells (intact mast cells); B: Type-Ⅱ cells (partially degranulated mast cells); C: Type-Ⅲ cells (completely degranulated mast cells)."

Figure 2

Effect of Bresol? on compound 48/80-induced mast cell degranulation All the values are expressed as mean ± standard error of mean, n=6; *P<0.05, vs normal control group; △P<0.05, vs positive control group."

Figure 3

Standard curve of histamine The standard curve for histamine was obtained by plotting the fluorescence intensity of histamine-o-phthaladehyde adduct against the concentration of histamine (μg/L)."

Figure 4

Effect of Bresol? on compound 48/80-induced histamine release from mast cells"

[1] Kay AB . Allergy and allergic diseases. First of two parts[J]. N Engl J Med, 2001,344(1):30-37
doi: 10.1056/NEJM200101043440106
[2] Kay AB . Allergy and allergic diseases. Second of two parts[J]. N Engl J Med, 2001,344(2):109-113
doi: 10.1056/NEJM200101113440206
[3] Singh R, Nath A, Gupta PP, Shulka M, Khare SK, Kundu B . Antiallergic/antiasthmatic activity of oligopeptide related to IgE[J]. Pharmacol Res, 1998,37(5):353-356
doi: 10.1006/phrs.1998.0302
[4] Muñoz S, Hernández-Pando R, Abraham SN, Enciso JA . Mast cell activation by Mycobacterium tuberculosis: mediator release and role of CD48[J]. J Immunol, 2003,170(11):5590-5596
doi: 10.4049/jimmunol.170.11.5590 pmid: 12759438
[5] Rajniti P . Efficacy and safety of Bresol® syrup in the management of allergic rhinitis: an open clinical study[J]. Antiseptic, 2010,107(7):345-350
[6] Chandrasekharan S, Paramesh R . Bresol® tablets in the management of nasal allergy and allergic rhinitis: an open, prospective trial[J]. Indian J Clin Pract, 2010,21(1):13-18
[7] Parmar BJ . Allergic respiratory disorders: role of Bresol®[J]. J Paediatr Obstetr Gynaecol, 2010,7:251-257
[8] Ikarashi Y, Yuzurihara M, Sakakibara I, Takahashi A, Ishimaru H, Maruyama Y . Effects of an oriental herbal medicine, “Saiboku-to”, and its constituent herbs on compound 48/80-induced histamine release from peritoneal mast cells in rats[J]. Phytomedicine, 2001,8(1):8-15
doi: 10.1078/0944-7113-00012
[9] Jeong HJ, Moon PD, Um JY, Park J, Leem KH, Kim CJ, Kim HM, Hong SH . Effect of Gamiseunggal-Tang on immediate type allergic reaction in mice[J]. Indian J Med Res, 2007,125(4):542-549
doi: 10.1038/sj.icb.7100029 pmid: 17598940
[10] Jung CH, Lee JY, Cho CH, Kim CJ . Anti-asthmatic action of quercetin and rutin in conscious guinea-pigs challenged with aerosolized ovalbumin[J]. Arch Pharm Res, 2007,30(12):1599-1607
doi: 10.1007/BF02977330
[11] Krishnaswamy G, Kelley J, Johnson D, Youngberg G, Stone W, Huang SK, Bieber J, Chi DS . The human mast cell: functions in physiology and disease[J]. Front Biosci, 2001,6:D1109-D1127
doi: 10.2741/krishnas pmid: 11532608
[12] Savali AS, Biradar PB, Jirankali MC . Antianaphylactic and mast cell stabilization activity of Cynodon dactylon[J]. Int J Pharm Pharm Sci, 2010,2(2):69-73
[13] Moon PD, Choi IY, Na HJ, Jeong HJ, Kim CH, Kim HR, Kim YK, Park SK, Hong SH, Kim HM . Rubus croceacanthus Leveille inhibits mast cell-mediated anaphylactic-like reaction and tumor necrosis factor-α secretion[J]. Biol Pharm Bull, 2004,27(9):1359-1363
doi: 10.1248/bpb.27.1359
[14] Patil UJ, Savali AS, Jirankali MC . Antianaphylactic and mast cell stabilization activity of Strychnos potatorum Linn. seed[J]. Pharmacognosy Res, 2011,3(3):208-213
doi: 10.4103/0974-8490.85011 pmid: 3193623
[15] Shahid M, Tripathi T, Sobia F, Moin S, Siddiqui M, Khan RA . Histamine, histamine receptors, and their role in immunomodulation: an updated systematic review[J]. Open Immunol J, 2009,2:9-41
doi: 10.2174/1874226200902010009
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