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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (5): 555-560.doi: 10.3736/jcim20120511

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice

Surendra Kr. Sharma(), Naveen Goyal#br#   

  1. Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, IndiaDepartment of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, India
  • Received:2011-12-05 Accepted:2012-02-15 Online:2012-05-20 Published:2018-06-15
  • Contact: Kr. Sharma Surendra E-mail:prof.sharmask@gmail.com

Objective: The aim of the present study was to evaluate the nephroprotective effect of methanolic extract of Heliotropium eichwaldii (MHE) in mice with cisplatin-induced acute renal damage.

Methods: Nephrotoxicity was induced by a single intraperitoneal injection of cisplatin (16 mg/kg). Swiss albino mice were injected with vehicle, cisplatin, cisplatin plus MHE 200 mg/kg and cisplatin plus MHE 400 mg/kg, respectively. MHE was administered for 7 d at a dose of 200 and 400 mg/kg per day orally starting 4 d before cisplatin injection. Animals were sacrificed 3 d after treatment and blood as well as kidney tissue was isolated and analyzed. The various parameters such as blood urea nitrogen (BUN), serum creatinine (CRE), malondialdehyde (MDA), and catalase (CAT) and superoxide dismutase (SOD) activities were analyzed.

Results: MHE treatment significantly reduced BUN and serum CRE levels elevated by cisplatin administration (P<0.05). Also, it significantly attenuated cisplatin-induced increase in MDA level and improved the decreased CAT and SOD activities in renal cortical homogenates (P<0.05). Additionally, histopathological examination and scoring showed that MHE markedly ameliorated cisplatin-induced renal tubular necrosis.

Conclusion: MHE can be considered a potential candidate for protection of nephrotoxicity induced by cisplatin.

Key words: Heliotropium, plant extracts, cisplatin, antioxidant, nephrotoxicity, mice

"

Group n BUN CRE
Normal control 6 360.0±23.0 6.0±0.4
Model control (vehicle plus cisplatin (16 mg/kg)) 6 2 340.0±116.0* 38.6±2.3*
MHE (200 mg/kg) plus cisplatin (16 mg/kg) 6 1 340.0±114.0 18.8±1.1
MHE (400 mg/kg) plus cisplatin (16 mg/kg) 6 960.0±63.0 9.3±0.8

"

Group n MDA (nmol/mg protein) SOD (U/mg protein) CAT (U/mg protein)
Normal control 6 0.36±0.03 46.3±3.3 13.3±1.0
Model control (vehicle plus cisplatin (16 mg/kg)) 6 0.92±0.08* 19.2±1.3* 6.1±0.5*
MHE (200 mg/kg) plus cisplatin (16 mg/kg) 6 0.81±0.07 28.6±3.1 8.6±0.7
MHE (400 mg/kg) plus cisplatin (16 mg/kg) 6 0.45±0.03 39.2±2.5 11.8±0.9

Figure 1

Photomicrographs of kidney tissues stained by hematoxylin and eosin (Light microscopy, ×100) A: Normal control group; B: Cisplatin plus vehicle-treated group showing tubular necrosis; C: Cisplatin plus MHE (400 mg/kg)-treated group displaying improvement in the histological appearance with marked reduction in tubular damage. MHE: methanolic extract of Heliotropium eichwaldi."

"

Group n Score
Normal control 6 0+
Model control (vehicle plus cisplatin (16 mg/kg)) 6 3+
MHE (200 mg/kg) plus cisplatin (16 mg/kg) 6 2+
MHE (400 mg/kg) plus cisplatin (16 mg/kg) 6 1+
[1] Joy J, Nair CK . Amelioration of cisplatin induced nephrotoxicity in Swiss albino mice by Rubia cordifolia extract[J]. J Cancer Res Ther, 2008,4(3):111-115.
doi: 10.4103/0973-1482.43139
[2] Lebwohl D, Canetta R . Clinical development of platinum complexes in cancer therapy: an historical perspective and an update[J]. Eur J Cancer, 1998,34(10):1522-1534.
doi: 10.1016/S0959-8049(98)00224-X
[3] Madias NE, Harrington JT . Platinum nephrotoxicity[J]. Am J Med, 1978,65(2):307-314.
doi: 10.1016/0002-9343(78)90825-2
[4] Taguchi T, Nazneen A, Abid MR, Razzaque MS . Cisplatin-associated nephrotoxicity and pathological events[J].Contrib Nephrol, 2005, 148: 107-121.
doi: 10.1159/000086055 pmid: 15912030
[5] Bodenner DL, Dedon PC, Keng PC, Katz JC, Borch RF . Selective protection against cis-diamminedichloroplatinum(Ⅱ)-induced toxicity in kidney, gut, and bone marrow by diethyldithiocarbamate[J]. Cancer Res, 1986,46(6):2751-2755.
[6] Babu E, Gopalakrishnan VK, Sriganth IN, Gopalakrishnan R, Sakthisekaran D . Cisplatin induced nephrotoxicity and the modulating effect of glutathione ester[J]. Mol Cell Biochem, 1995,144(1):7-11.
doi: 10.1007/BF00926734 pmid: 7791748
[7] Chirino YI, Pedraza-Chaverri J . Role of oxidative and nitrosative stress in cisplatin-induced nephrotoxicity[J]. Exp Toxicol Pathol, 2009,61(3):223-242.
doi: 10.1016/j.etp.2008.09.003 pmid: 18986801
[8] Ali BH, Al Moundhri MS . Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research[J]. Food Chem Toxicol, 2006,44(8):1173-1183.
doi: 10.1016/j.fct.2006.01.013
[9] Jain SC, Sharma R . Antimicrobial activity of pyrrolizidine alkaloids from Heliotropium ellipticum. Chem Pharm Bull (Tokyo). 1987; 35(8):3487-3489.
doi: 10.1248/cpb.35.3487 pmid: 3427725
[10] Reina M, Gonzalez-Coloma A, Gutierrez C, Cabrera R, Henriquez J, Villarroel L . Bioactive saturated pyrrolizidine alkaloids from Heliotropium floridum. Phytochemistry. 1997; 46(5):845-853.
doi: 10.1016/S0031-9422(97)00354-3
[11] Rastoqi RP, Mehrotra BN . A compendium on Indian medicinal plants. Vol 2. New Delhi and Lucknow: Publications & Information Directorate . 1991.
[12] Chopra RN, Nayar SL, Chopra IC . Glossary of Indian medicinal plants. 3rd ed. New Delhi: Council of Scientific & Industrial Research. 1956.
[13] Kirtikar KR, Basu BD . Indian medicinal plants. 2nd ed. Dehradun: International Book Distributors. 1991.
[14] Bhakuni DS, Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN . Screening of Indian plants for biological activity. Ⅱ. Indian[J] Exp Biol. 1969; 7(4):250-262
[15] Gupta SK, Mathur IS . The effect of Arnebia nobilis and its naphthaquinones in rat Walker carcinosarcoma 256[J]. Indian J Cancer, 1972,9(1):50-55.
pmid: 5044709
[16] Jain SC, Singh B . Bioefficacy of Heliotropium ellipticum Ledeb. Ⅰ. Antimicrobial screening[J]. Indian J Pharm Sci, 1998,60(6):394-396.
[17] Sheena N, Ajith TA, Janardhanan KK . Prevention of nephrotoxicity induced by the anticancer drug cisplatin, using Ganoderma lucidum, a medicinal mushroom occurring in South India[J]. Curr Sci, 2003,85(4):478-482.
[18] Varley H, Gowenlock Alen H , Bell M. Practical clinical biochemistry.5th ed[M].London: William Heinmamm Medical Books Ltd, 1980: 456-460, 478-480.
[19] Ohkawa H, Ohishi N, Yagi K . Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J]. Anal Biochem, 1979,95(2):351-358.
doi: 10.1016/0003-2697(79)90738-3
[20] McCord JM, Fridovich I . Superoxide dismutase. An enzymic function for erythrocuprein(hemocuprein)[J]. J Biol Chem, 1969,244(22):6049-6055. x
[21] Beers RF Jr, Sizer IW . A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase[J]. J Biol Chem, 1952,195(1):133-140.
[22] Aebi H.Catalase in vitro[J].Methods Enzymol, 1984, 105: 121-126.
doi: 10.1016/S0076-6879(84)05016-3
[23] Ramesh G , Reeves WB.p38 MAP kinase inhibition ameliorates cisplatin nephrotoxicity in mice[J]. Am J Physiol Renal Physiol, 2005,289(1):F166-F174.
doi: 10.1152/ajprenal.00401.2004 pmid: 15701814
[24] Yao X, Panichpisal K, Kurtzman N, Nugent K . Cisplatin nephrotoxicity: a review[J]. Am J Med Sci, 2007,334(2):115-124.
doi: 10.1097/MAJ.0b013e31812dfe1e
[25] Hannemann J, Baumann K . Cisplatin-induced lipid peroxidation and decrease of gluconeogenesis in rat kidney cortex: different effects of antioxidants and radical scavengers[J]. Toxicology, 1988,51(2-3):119-132.
doi: 10.1016/0300-483X(88)90143-6
[26] Uslu R, Bonavida B . Involvement of the mitochondrion respiratory chain in the synergy achieved by treatment of human ovarian carcinoma cell lines with both tumor necrosis factor-α and cis-diamminedichloroplatinum[J]. Cancer, 1996,77(4):725-732.
doi: 10.1002/(ISSN)1097-0142
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