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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (5): 555-560.doi: 10.3736/jcim20120511

• Original Experimental Research • Previous Articles     Next Articles

Protective effect of Heliotropium eichwaldi against cisplatin-induced nephrotoxicity in mice

Surendra Kr. Sharma(), Naveen Goyal#br#   

  1. Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, IndiaDepartment of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001, Haryana, India
  • Received:2011-12-05 Accepted:2012-02-15 Online:2012-05-20 Published:2018-06-15
  • Contact: Kr. Sharma Surendra E-mail:prof.sharmask@gmail.com

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Objective: The aim of the present study was to evaluate the nephroprotective effect of methanolic extract of Heliotropium eichwaldii (MHE) in mice with cisplatin-induced acute renal damage.

Methods: Nephrotoxicity was induced by a single intraperitoneal injection of cisplatin (16 mg/kg). Swiss albino mice were injected with vehicle, cisplatin, cisplatin plus MHE 200 mg/kg and cisplatin plus MHE 400 mg/kg, respectively. MHE was administered for 7 d at a dose of 200 and 400 mg/kg per day orally starting 4 d before cisplatin injection. Animals were sacrificed 3 d after treatment and blood as well as kidney tissue was isolated and analyzed. The various parameters such as blood urea nitrogen (BUN), serum creatinine (CRE), malondialdehyde (MDA), and catalase (CAT) and superoxide dismutase (SOD) activities were analyzed.

Results: MHE treatment significantly reduced BUN and serum CRE levels elevated by cisplatin administration (P<0.05). Also, it significantly attenuated cisplatin-induced increase in MDA level and improved the decreased CAT and SOD activities in renal cortical homogenates (P<0.05). Additionally, histopathological examination and scoring showed that MHE markedly ameliorated cisplatin-induced renal tubular necrosis.

Conclusion: MHE can be considered a potential candidate for protection of nephrotoxicity induced by cisplatin.

Key words: Heliotropium, plant extracts, cisplatin, antioxidant, nephrotoxicity, mice

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Group n BUN CRE
Normal control 6 360.0±23.0 6.0±0.4
Model control (vehicle plus cisplatin (16 mg/kg)) 6 2 340.0±116.0* 38.6±2.3*
MHE (200 mg/kg) plus cisplatin (16 mg/kg) 6 1 340.0±114.0△ 18.8±1.1△
MHE (400 mg/kg) plus cisplatin (16 mg/kg) 6 960.0±63.0△ 9.3±0.8△

"

Group n MDA (nmol/mg protein) SOD (U/mg protein) CAT (U/mg protein)
Normal control 6 0.36±0.03 46.3±3.3 13.3±1.0
Model control (vehicle plus cisplatin (16 mg/kg)) 6 0.92±0.08* 19.2±1.3* 6.1±0.5*
MHE (200 mg/kg) plus cisplatin (16 mg/kg) 6 0.81±0.07 28.6±3.1△ 8.6±0.7△
MHE (400 mg/kg) plus cisplatin (16 mg/kg) 6 0.45±0.03△ 39.2±2.5△ 11.8±0.9△

Figure 1

Photomicrographs of kidney tissues stained by hematoxylin and eosin (Light microscopy, ×100) A: Normal control group; B: Cisplatin plus vehicle-treated group showing tubular necrosis; C: Cisplatin plus MHE (400 mg/kg)-treated group displaying improvement in the histological appearance with marked reduction in tubular damage. MHE: methanolic extract of Heliotropium eichwaldi."

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Group n Score
Normal control 6 0+
Model control (vehicle plus cisplatin (16 mg/kg)) 6 3+
MHE (200 mg/kg) plus cisplatin (16 mg/kg) 6 2+
MHE (400 mg/kg) plus cisplatin (16 mg/kg) 6 1+
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