L-arginine methyl ester,Hypertensive,Tridax procumbens,Corpus cavernosum,Relaxation,Sodium nitroprusside,Acetylcholine," /> L-arginine methyl ester,Hypertensive,Tridax procumbens,Corpus cavernosum,Relaxation,Sodium nitroprusside,Acetylcholine,"/> L-arginine methyl ester,Hypertensive,Tridax procumbens,Corpus cavernosum,Relaxation,Sodium nitroprusside,Acetylcholine
,"/> <span><span id="__kindeditor_bookmark_start_6__"></span>Effects of aqueous leaf extract of <em>Tridax procumbens</em> on contractile activity of corpus cavernosum in <em>N</em>-nitro-<span style="font-size:10px;">L</span>-arginine methyl ester-induced hypertensive male rats</span><span><span id="__kindeditor_bookmark_end_7__"></span></span>
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Journal of Integrative Medicine ›› 2018, Vol. 16 ›› Issue (1): 51-56.doi: 10.1016/j.joim.2017.11.001

• Original Experimental Research • Previous Articles     Next Articles

Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester-induced hypertensive male rats

Shakiru Ademola Salami a, Hussein Mofomosara Salahdeen a, Evangelshane Chukwudubem Ugbebor a, Babatunde Adekunle Murtala a, Yinusa Raji b   

  1. a Department of Physiology, Lagos State University College of Medicine, Lagos State, Ikeja 23401, Nigeria
    b Laboratory for Reproductive Physiology and Developmental Programming, Department of Physiology, University of Ibadan, Ibadan 23402, Nigeria

  • Received:2017-04-20 Accepted:2017-08-25 Online:2018-01-15 Published:2017-11-26
  • Contact: Shakiru Ademola Salami; E-mail: piety1424@yahoo.com

Objective
This study investigated the effects of aqueous leaf extract of Tridax procumbens (ALETP) on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive male rats.


Methods

Twenty normal, adult male rats (130–150?g) were divided into four groups of five rats each. Group I (control) was given normal saline (0.6?mL/kg) and group II was given L-NAME (40?mg/kg) for 6?weeks. Groups III and IV also received L-NAME (40?mg/kg) for 6?weeks but were further co-treated with 100 and 200?mg/kg of ALETP, respectively, from week 4 to week 6. All treatments were given orally. Strips of corpus cavernosum from each of the four groups were exposed to increasing concentrations of acetylcholine (ACh) and sodium nitroprusside (SNP) (10?9–10?5 mol/L) after contraction with phenylephrine (10?7?mol/L) to test for a dose–response effect. Response to potassium and calcium was also measured after cumulatively adding potassium and calcium (10–50?mmol/L) to potassium- and calcium-free organ chamber. Isometric contractions were recorded through an Ugo Basile data capsule acquisition system.


Results

Mean arterial blood pressure was significantly reduced in the ALETP co-treated group compared to the control and L-NAME-only groups (P?<?0.05). Cavernosa strips from ALETP co-treated rats exhibited significant inhibition of contraction in response to phenylephrine, potassium chloride, and calcium chloride (P?<?0.05). Relaxation in response to Ach and SNP was also significantly impaired in cavernosa strips from the L-NAME-only treated group (P?<?0.05), while ALETP co-treated groups showed enhanced percentage relaxation.


Conclusion

ALETP treatment of L-NAME-induced hypertensive rats promotes a relaxant effect on isolated cavernosa strips. ALETP shows potential in correcting erectile dysfunction in hypertension.

Key words: L-arginine methyl ester')">N-Nitro-L-arginine methyl ester, Hypertensive, Tridax procumbens, Corpus cavernosum, Relaxation, Sodium nitroprusside, Acetylcholine

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