Search JIM Advanced Search

Journal of Integrative Medicine ›› 2018, Vol. 16 ›› Issue (4): 283-289.doi: 10.1016/j.joim.2018.04.008

• Original Experimental Research • Previous Articles     Next Articles

Feiji Recipe inhibits the growth of lung cancer by modulating T-cell immunity through indoleamine-2,3-dioxygenase pathway in an orthotopic implantation model

Bin Luo a, 1, Zu-jun Que b, 1, Zhi-yi Zhou a, Qing Wang b, Chang-sheng Dong b, Yi Jiang a, Bing Hu a, b, Hui Shi c, Yu Jin c, Jian-wen Liu c, He-gen Li a, Lin Wang d, Jian-hui Tian a,b   

  1. a Department of Oncology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b Oncology Institute of Traditional Chinese Medicine, Institute of TCM Oncology, Shanghai 200032, China
    c School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
    d Department of Nephrology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2017-09-26 Accepted:2018-03-15 Online:2018-07-09 Published:2018-04-22
  • Contact: Lin Wang; E-mail: happytlynn@163.com Jian-hui Tian; E-mail: hawk7150@hotmail.com

Objective
Escape from the body’s immune response is a basic characteristic of lung cancer, and indoleamine-2,3-dioxygenase (IDO) plays a key role in mediating immune escape of non-small-cell lung cancer, which leads to recurrence and metastasis. Feiji Recipe, a compound Chinese herbal medicine, has the effect of stabilizing lesions and prolonging survival in patients with lung cancer. The purpose of this study was to investigate the mechanisms underlying the anticancer properties of Feiji Recipe.


Methods

An orthotopic transplant model of mouse Lewis lung cancer, with stable expression of IDO gene, was established in C57BL/6 mice. Optical imaging was used to observe the effects of Feiji Recipe in the treatment of lung cancer in vivo. The effects of Feiji Recipe on the proliferation of mouse Lewis lung cancer cell line 2LL, 2LL-enhanced green fluorescent protein (2LL-EGFP) and 2LL-EGFP-IDO were investigated, and the apoptosis of T-cells was examined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide using flow cytometry. Chemical composition of Feiji Recipe was validated by high-performance liquid chromatography.


Results

Compared to the control group, the survival of animals treated with Feiji Recipe was significantly prolonged (P?=?0.0074), and the IDO protein level decreased (P?=?0.0072); moreover, the percentages of CD4+CD25+ T-cells and Foxp3+ T-cells were significantly decreased (P?<?0.05). The molecular mechanism of Feiji Recipe against lung cancer may relate to the regulation of immune cells, such as T-cells and regulatory T-cells.


Conclusion

The molecular mechanism of Feiji Recipe in treatment of lung cancer is to restore the function of T-cells in the cancer microenvironment through interfering with the IDO pathway.

Key words: Feiji Recipe, Immune escape, Orthotopic xenografts, Indoleamine-2,3-dioxygenase

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[3] Peng-fei Gao, Kenji Watanabe. Introduction of the World Health Organization project of the International Classification of Traditional Medicine. Journal of Chinese Integrative Medicine, 2011, 9(11): 1161-1164
[4] Yi Chen, Wen-jian Wang. Relationships between insulin resistant and hypertension and traditional Chinese medicine intervention. Journal of Chinese Integrative Medicine, 2010, 8(12): 1114-1118
[5] Kai Qu, Tian-fang Wang, Yan Zhao, Xiao-lin Xue, Xiu-yan Wu, Hua-liang Deng, Lu Yang, Li-liYu , Hong Yue, Qing-guo Wang. Common syndrome factors of traditional Chinese medicine in chronic renal failure based on the questionnaire investigation among experts. Journal of Chinese Integrative Medicine, 2010, 8(12): 1147-1152
[6] Mei-jun Lü, Hui-yong Zhang, Zhe Zhang, Guan-lin Yang. Measurement of health-related quality of life in coronary heart disease: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1277-1285
[7] Shuo Zhang , Hong-cai Shang. Correlation between traditional Chinese medicine syndromes and omics: A literature review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1286-1291
[8] Qin-hui Fu, Jian Pei, Jian-rong Hui, Yi Song . Studies on dynamic changes in traditional Chinese medicine syndrome patterns for stroke using data-driven and model-driven approaches: A review. Journal of Chinese Integrative Medicine, 2011, 9(12): 1292-1300
[9] Jia Guo, Li-na Wang, Dong Li. Exploring the effects of Chinese medicine in improving uterine endometrial blood flow for increasing the successful rate of in vitro fertilization and embryo transfer. Journal of Chinese Integrative Medicine, 2011, 9(12): 1301-1306
[10] Jian-sheng Li, Su-yun Li, Xue-qing Yu, Yang Xie. Bufei Yishen Granule combined with acupoint-sticking therapy in patients with stable chronic obstructive pulmonary disease: Study protocol of a multicenter, randomized, double-blind, active-controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(12): 1312-1318