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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 338-342.doi: 10.3736/jcim20070321

• Original Experimental Research • Previous Articles     Next Articles

Expressions of epidermal growth factor receptor signaling substances in gastric mucosal cells influenced by serum derived from rats treated with electroacupuncture at stomach meridian acupoints

Jie Yan, Zong-bao Yang, Xiao-rong Chang, Shou-xiang Yi, Ya-ping Lin, Yan Zhong   

  1. College of Acupuncture and Massage , Hunan University of T raditional Chinese Medicine , Changsha , Hunan Province 410007 ,China
  • Online:2007-05-31 Published:2007-05-15

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Objective: To study the effects of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expressions of epidermal growth factor receptor (EGFR) signaling substances phospholipase Cγ-1 (PLCγ-1), protein kinase C (PKC) and c-myc in gastric mucosal cells.Methods: Sixty rats were randomly divided into normal group, stomach meridian group, gallbladder meridian group, stomach meridian plus PD153035 group and gallbladder meridian plus PD153035 group. Water-immersion and restrained stress methods were adopted for inducing gastric mucosal injury in the rats. Gastric mucosal cells were separated by using pronase digestion method, and incubated by PD153035, a EGFR inhibitor, and 100 ml/L serum. The expression of PLCγ-1 in the gastric mucosal cells was tested by enzyme linked-immunosorbent assay (ELISA), while the expression of PKC by isotope incorporate assay and the expression of c-myc by reverse transcription polymerase chain reaction assay (RT-PCR).Results: In gastric mucosal cells, weak expressions of PLCγ-1, PKC and c-myc were seen in the normal group, and relatively strong expressions of PLCγ-1, PKC and c-myc were seen in the stomach meridian group and the gallbladder meridian group, among which, the expressions of PLCγ-1, PKC and c-myc in the stomach meridian group were the strongest, and there was a significant difference between the stomach meridian group and the gallbladder meridian group (P＜0.01). Relative weak expressions of PLCγ-1, PKC and c-myc were seen in the stomach meridian plus PD153035 group and the gallbladder meridian plus PD153035 group, and there was a significant difference between the stomach meridian group and the stomach meridian plus PD153035 group (P＜0.01).Conclusions: The serum derived from the rats treated with electroacupuncture at stomach meridian acupoints can activate the EGFR singling pathway, and this provides an evidence for the theory of "relative particularity between meridians and viscera" in traditional Chinese medicine.

Table 1

Expressions of PKC, c-myc gene and PLCγ-1 in gastric mucosal cells in 5 groups（ヌ±S）"

GroupnPLCγ-1[nmol/(g·min)]PKC[nmol/(g·min)]c-myc gene
Normal8390.45±120.8417.49±18.560.804 2±0.070 5
Stomach meridian8778.90±449.78**68.94±39.36**1.089 4±0.022 7**
Gallbladder meridian8503.84±232.40**△△21.88±16.52△△1.004 2±0.033 3**△△
Stomach meridian + PD1530358332.32±119.20**△△▲▲10.43±14.03△△0.920 0±0.034 3**△△▲
Gallbladder meridian + PD1530358430.46±107.98**△△▲▲14.06±8.65△△0.911 4±0.038 5**△△▲▲

Figure 1

Electrophoretogram of c-myc gene and GAPDH gene RT-PCR product in gastric mucosal cellsM: Marker; A: Normal group; B: Stomach meridian group; C: Gallbladder meridian group; D: Stomach meridian + PD153035 group; E: Gallbladder meridian + PD153035 group."

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