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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 333-337.doi: 10.3736/jcim20070320

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High-protein diet and high-grade proteinuria aggravate the progression of chronic renal failure in rats

Li-qun He1, Jun Li2, Qi Li1   

  1. 1. Department of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China
    2. The Fifth Hospital, Zunyi College of Medicine, Zunyi, Guizhou Province 563000, China
  • Online:2007-05-31 Published:2007-05-15

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Objective: To study the effects of high-protein diet (HPD) and high-grade proteinuria in aggravating the progression of chronic renal failure (CRF) in rats.Methods: CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine (Scr), blood urea nitrogen (BUN), endothelin-1 (ET-1), thromboxane B2 (TXB2) and 6-keto-prostaglandin Flα (6-Keto-PGFlα) were observed. At the same time, the content of malondialdehyde (MDA), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase（SOD）were measured in blood and renal tissue of the rats.Result: HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB2, reducing the level of 6-Keto-PGFlα, and attenuating the activities of SOD and GSH-Px.Conclusion: HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the anti-oxidation.

Key words: kidney failure, chronic, protein, dietary, proteinuria, hemodynamics, rats

CLC Number: 

  • R692.5

Table 1

Effects of protein diet on renal function and proteinuria in CRF rats （ヌ±S）"

Group n Scr (μmol/L) BUN (mmol/L) 24 h urine pro (mg/24 h)
Normal control 7 78.90±40.42 7.93±4.35 14.32±2.04
Untreated 8 215.78±58.07**△△ 19.73±5.24△△ 151.24±30.77**△△
Middle-protein diet 7 231.90±47.79*△△ 24.10±5.28△△ 174.00±16.85**△△
High-protein diet 7 315.00±77.32△△ 25.54±6.14△△ 248.08±66.19△△
F 19.71 15.97 43.62

Table 2

Effects of protein diet on vasoactive substances in CRF rats （ヌ±S）"

Group n ET-1 (ng/L) TXB2 (ng/L) 6-Keto-PGFlα (ng/L)
Normal control 7 62.24±33.06 180.65±26.58 8.40±1.16
Untreated 8 135.74±33.58△△ 253.26±36.37*△ 5.12±1.55△△
Middle-protein diet 7 141.35±37.62△△ 238.07±53.21*△ 4.41±1.15△△
High-protein diet 7 152.22±63.78△△ 304.30±13.63△△ 4.09±1.83△△
F 6.11 11.63 11.12

Table 3

Effects of protein diet on oxidation indexes of blood in CRF rats（ヌ±S）"

Group n SOD (kU/L) MDA (μmol/L) GSH-Px (mmol/L)
Normal control 7 49.50±3.10 8.60±2.80 2.01±0.32
Untreated 8 41.30±4.60*△ 22.20±3.80*△△ 0.81±0.11*△△
Middle-protein diet 7 37.70±8.70△△ 24.80±5.20△△ 0.61±0.22△△
High-protein diet 6 34.10±7.50△△ 27.70±6.60△△ 0.38±0.10△△
F 7.40 21.60 81.50

Table 4

Effects of protein diet on oxidation indexes of renal tissue in CRF rats （ヌ±S）"

Group n SOD (U/ml) MDA (nmol/ml) GSH-Px (mmol/L)
Normal control 7 37.84±5.99 4.28±3.81 3.06±1.45
Untreated 8 32.67±5.53 9.68±4.77△ 1.57±0.56△
Middle-protein diet 7 23.67±7.23*△△ 10.58±5.60△ 1.75±0.71△
High-protein diet 6 28.90±2.70△△ 12.39±5.23△△ 1.65±0.89△
F 7.64 4.56 3.55
[1] 陈香梅, 徐启何, 王梅 , 等. 现代慢性肾衰治疗学(第1版)[M]. 北京: 人民军医出版社, 2001,( 第1版):227-227
Cheng XM, Xu QH, Wang M , et al. Modern chronic renal failure acology(1st ed)[M]. Beijing: People's Military Medical Press, 2001,( 1st ed):227-227
[2] 何立群, 郑平东, 陈刚 . 大鼠肾大部切除诱发慢性肾衰模型的建立[J]. 上海实验动物科学, 2000,20(1):11-13
He LQ, Zheng PD, Chen G . Rat model of chronic renal failure induced by subtotal nephrectomy[J]. Shanghai Shi Yan Dong Wu Ke Xue, 2000,20(1):11-13
[3] Woo KT, Lau YK . Proteinuria: clinical significance and basis for therapy[J]. Singapore Med J, 2001,42(8):385-389
pmid: 11764058
[4] Remuzzi G, Ruggenenti P, Benigni A . Understanding the nature of renal disease progression[J]. Kidney Int, 1997,51(1):2-15
[5] Perico N, Remmuzi G . Role of endothelin in glomerular injury[J]. Kidney Int (Suppl), 1993,39(1):76-80
pmid: 8468930
[6] Hocher B, Thone-Reineke C, Rohmeiss P , et al. Endothelin-1 transgenic mice develop glomerulosclerosis, interstitial fibrosis, and renal cysts but not hypertension[J]. J Clin Invest, 1997,99(6):1380-1389
[7] Naumnik B, Borawski J, Pawlak K , et al. Renal function, proteinuria and ACE-inhibitor therapy as determinants of plasma levels of endothelial markers[J]. Nephrol Dial Transplant, 2002,17(3):526-528
[8] Ruggenenti P, Gaspari F, Perna A , et al. Cross sectional longitudinal study of spot morning urine protein: creatinine ratio, 24 hour urine protein excretion rate, glomerular filtration rate, and end stage renal failure in chronic renal disease in patients without diabetes[J]. BMJ, 1998,316(7130):504-509
[9] Whinnery MA, Shaw JO, Beck N . Thromboxane B2 and prostaglandin E2 in the rat kidney with unilateral ureteral obstruction[J]. Am J Physiol, 1982,242(3):220-225
doi: 10.1152/ajprenal.1982.242.3.F220 pmid: 6950670
[10] Stahl RA, Kudelka S, Helmchen U . High protein intake stimulates glomerular prostaglandin formation in remnant kidneys[J]. Am J Physiol, 1987,252(6 Pt 2):1088-1094
doi: 10.1152/ajprenal.1987.252.6.F1088 pmid: 3473940
[11] 廖新波, 彭杰青, 熊密 , 等. 氧自由基在肾小球肾炎发病机制中的意义[J]. 广东医学院学报, 1996,14(4):307-308,317
Liao XB, Peng JQ, Xiong M , et al. The significance of oxygen free radicals in pathogenesis of antiglomerular basement membrane glomerulonephritis[J]. Guangdong Yi Xue Yuan Xue Bao, 1996,14(4):307-308, 317
[12] Nakamura K, Oka M, Shirai M , et al. Source of reactive oxygen species in anti-Thy1 nephritis[J]. Ren Fail, 1998,20(2):399-405
[13] Border WA, Okuda S, Languino LR , et al. Transforming growth factor-beta regulates production of proteoglycans by mesangial cells[J]. Kidney Int, 1990,37(2):689-695
[14] 唐政, 黎磊石 . 氧自由基及抗氧化酶与慢性肾脏疾病的进展[J]. 国外医学·泌尿系统分册, 1990,19(4):185-189
Tang Z, Li LS . Research progress of association between oxygen free radicals, antioxidase and chronic kidney diseases[J]. Guo Wai Yi Xue Mi Niao Xi Tong Fen Ce, 1990,19(4):185-189
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