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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (6): 563-568.doi: 10.3736/jcim20090613

Special Issue: Acupuncture & Moxibustion

• Original Experimental Research • Previous Articles     Next Articles

Effects of electro-acupuncture on signal transduction pathway of hypothalamic neuroendocrine system in ovariectomized rats

Feng Guan, Shu-lan Ma, Bo-ying Chen   

  1. Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, Shanghai 200032, China
  • Received:2009-02-03 Accepted:2009-03-20 Online:2009-06-20 Published:2009-06-15


To compare the varieties and contents of the main nerval information molecules in perfusate from hypothalamic medial preoptic area (MPOA) of the rats in different sexual cycles and the ovariectomized rats treated by electro-acupuncture, so as to observe the similarities and differences of hypothalamic neuroendocrine signal transduction pathway under the physiological and pathological status, and to explore the mechanisms of neuroendocrine signal transduction of electro-acupuncture therapeutic effect in perimenopausal syndrome.

The stereo localization technique and push-and-pull perfusion of the rat brain nucleus were adopted for collecting the hypothalamic MPOA perfusate of the female rats with normal sexual cycle, and also for collecting the MPOA perfusate of ovariectomized rats after electro-acupuncture treatment as acupuncture perfusate (AP). After being respectively microinjected into MPOA of the ovariectomized rats, the influence of the different perfusates on vagina cytology and serum estradiol (E2) level was observed. The contents of gonadotropin-releasing hormone (GnRH), dopamine (DA), γ-aminobutyric acid (GABA), glutamate (Glu), aspartate (Asp) and β-endorphin (β-EP) in the perfusate of each group were detected by radioimmunoassay or high performance liquid chromatography, and then the varieties and contents of these substances in the perfusate of each group were compared and analyzed.

The contents of neural active substances including DA, GABA, Glu, and β-EP in the perfusate from the rats’ MPOA during different stages of sexual cycle showed some regular changes. After the perfusate was microinjected respectively into the MPOA of the ovariectomized rats, the changes of animal vaginal exfoliated cells and serum E2 level showed the similar four-stage cycle characteristics as normal rats; the changes of vaginal exfoliated cells and serum E2 level of the ovariectomized rats without electro-acupuncture treatment showed the acupuncture-like effects following the microinjection of AP in the MPOA.

The information molecules in the perfusate from MPOA of rats in normal sexual cycle or electro-acupuncture-treated rats include classical neurotransmitters, amino acid neurotransmitters, and neuropeptides. Acupuncture may modulate the release and synthesis of these information molecules to normalize the subnormal function of MPOA in perimenopausal period. Thus, AP may be a neuroendocrine signal carrier of acupuncture effects for acupuncture signal transduction from one individual to another. This may provide a new thinking and method for studying acupuncture signal transduction of the acupuncture therapeutic effects on perimenopausal syndrome.

Key words: Acupuncture, Electro-acupuncture gonadotropin releasing hormone, Sexual cycle, Signal transduction, Rats

Figure 1

Content of serum E2 of rats in different groups **P<0.01, vs OVXP group. Data were represented as x±s, n=6."

Figure 2

Exfoliative epitheliums of vaginal smears of rats in different groups A: OVXP group; B: IAP group; C: IPEP group; D: IEP group; E: IMEP group; F: IDEP group."

Table 1

Contents of the nerval activity substances in hypothalamic MPOA perfusate in different groups(x±s)"

Group n GABA (ng/L) β-EP (ng/L) DA (nmol/L) Asp (μg/L) Glu (μg/L) GnRH (ng/L)
OVXP 6 444±84 58.84±15.28 2.03±0.57 418±68 1 759±178 81.50±8.29
DEP 6 484±79 81.14±29.11 3.64±1.09* 247±37* 1 540±208 61.47±9.92
PEP 6 497±98 84.24±31.05 3.62±0.97* 299±35* 674±106** 76.25±7.44
EP 6 437±85 113.12±30.08* 4.03±1.12** 133±21** 587±98** 79.84±8.57
MEP 6 681±105* 73.19±20.35 3.01±0.83 353±40 1 232±213 56.32±8.68*
AP 6 836±156** 139.81±36.44** 4.46±1.21** 278±64** 978±105** 54.66±13.07*
[1] Chen BY, Yu J . Relationship between blood radioimmunoreactive beta-endorphin and hand skin temperature during the electro-acupuncture induction of ovulation[J]. Acupunct Electrother Res, 1991,16(1-2):1-5
[2] Zhao H, Tian Z, Hao J, Chen B . Extragonadal aromatization increases with time after ovariectomy in rats[J]. Reprod Biol Endocrinol, 2005,( 3):6
[3] Zhao H, Tian Z, Cheng L, Chen B . Electroacupuncture enhances extragonadal aromatization in ovariectomized rats[J]. Reprod Biol Endocrinol, 2004,( 2):18
[4] Chen BY . Acupuncture normalizes dysfunction of hypothalamic-pituitary-ovarian axis[J]. Acupunct Electrother Res, 1997,22(2):97-108
doi: 10.3727/036012997816356734
[5] Zhao H, Tian ZZ, Chen BY . An important role of corticotropin-releasing hormone in electroacupuncture normalizing the subnormal function of hypothalamus-pituitary-ovary axis in ovariectomized rats[J]. Neurosci Lett, 2003,349(1):25-28
doi: 10.1016/S0304-3940(03)00676-1
[6] Cheng LN, Du GZ, Chen BY . The biochemical mechanism of electroacupuncture regulation of dysfunctional hypothalamic-pituitary-ovarian axis in ovariectomized rats[J]. Shanghai Zhen Jiu Za Zhi, 2001,20(6):32-34
程丽娜, 杜桂珍, 陈伯英 . 电针调整去卵巢大鼠下丘脑-垂体-卵巢轴异常功能的生化机制[J]. 上海针灸杂志, 2001,20(6):32-34
[7] Hao JW, Ma SL, Jiang JW, Chen BY . A study of in vitro effects by nucleus microinjection of acupuncture push-pull perfusion fluid from preoptic area of ovariectomized rats[J]. Shanghai Zhen Jiu Za Zhi, 2006,25(9):46-50
郝俊伟, 马淑兰, 姜建伟, 陈伯英 . 核团微量注射下丘脑视前区"针刺灌流液"对去卵巢大鼠整体效应的研究[J]. 上海针灸杂志, 2006,25(9):46-50
[8] Smith MJ, Jennes L . Neural signals that regulate GnRH neurones directly during the oestrous cycle[J]. Reproduction, 2001,122(1):1-10
doi: 10.1530/rep.0.1220001
[9] Gao H, Ji SZ, Chen BY . Electroacupuncture promotes enlargement of adrenals and enhances level of blood corticosterone in ovariectomized rats[J]. Zhen Ci Yan Jiu, 1995,20(2):55-58
高慧, 季士珠, 陈伯英 . 电针促进去卵巢大鼠肾上腺增大, 血皮质酮含量升高[J]. 针刺研究, 1995,20(2):55-58
[10] Che P, Zhao H, Chen BY . Ectopic expression of GnRH during natural compensation of HPOA function and the effect of electroacupuncture on it in ovariectomized rats[J]. Shanghai Zhen Jiu Za Zhi, 2007,26(11):39-42
车萍, 赵宏, 陈伯英 . 去卵巢大鼠HPOA功能自然代偿过程中GnRH的异位表达及电针处理对其的影响[J]. 上海针灸杂志, 2007,26(11):39-42
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