儿茶素对5/6肾切除大鼠肾脏微血管的保护作用及其机制

doi:10.3736/jcim20090612

Abstract

Objective

To investigate the effects of catechin on angiotensin-converting enzyme (ACE) activity, angiotensin Ⅱ(Ang Ⅱ) content and microvessel density (MVD) in renal tissues of 5/6 nephrectomized rats.
Methods

Sixty SD rats were divided into 3 groups: sham-operated group, untreated group and catechin group. Animal model was reproduced by 5/6 nephrectomy. After 8- and 12-week administration, rats were sacrificed. Renal MVD was measured by immunohistochemical method with CD34 marking. Activities of ACE in plasma and renal cortices were measured by ultraviolet spectrophotometer, and Ang Ⅱ contents in plasma and renal cortices were measured by radioimmunoassay. Glomerular sclerosis index (GSI) and tubule interstitial score (TIS) were calculated by semiquantitative integration with hematoxylin and eosin staining and periodic acid-Schiff staining.
Results

After 8- and 12-week administration, the GSI and TIS in the catechin group were much less than those in the untreated group (P<0.05, P<0.01). MVDs around glomerulus and tubule at the end of the 8th and 12th week in the untreated group and the catechin group were much less than those in the sham-operated group (P<0.01). The ACE activities and Ang Ⅱcontents in plasma and renal cortices in the catechin group were much less than those in the untreated group (P<0.01). By Pearson correlation analysis, we found that MVD had negative correlation with GSI, TIS, Ang Ⅱ content, and ACE activity(P<0.01), however, the ACE activity and Ang Ⅱ content had positive correlation with GSI, TIS (P<0.01).
Conclusion

Catechin can prevent the 5/6 nephrectomized rats from decreasing of MVD and inhibit the progress of glomerular sclerosis and interstitial fibrosis by inhibiting the activity of ACE and reducing the production of Ang Ⅱ.

Key words: Catechin, Microvessel density, Angiotensin II, Angiotensin-converting enzyme, Rats

Yan Cao, Xiao-jie He, Wei Xiang, Zhu-wen Yi . Protective effect of catechin on renal microvessels in 5/6 nephrectomized rats and its mechanism. Journal of Chinese Integrative Medicine, 2009, 7(6): 557-562.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20090612

http://www.jcimjournal.com/EN/Y2009/V7/I6/557

TrendMD

Figures/Tables 6

Figure 1

Table 1

Figure 2

Table 2

Table 3

Table 4

References 17

[1]	Ohashi R, Shimizu A, Masuda Y, Kitamura H, Ishizaki M, Sugisaki Y, Yamanaka N . Peritubular capillary regression during the progression of experimental obstructive nephropathy[J]. J Am Soc Nephrol, 2002,13(7):1795-1805 doi: 10.1097/01.ASN.0000018408.51388.57
[2]	Razzaque MS, Taguchi T . Cellular and molecular events leading to renal tubulointerstitial fibrosis[J]. Med Electron Microsc, 2002,35(2):68-80 doi: 10.1007/s007950200009
[3]	Kang DH, Kanellis J, Hugo C, Truong L, Anderson S, Kerjaschki D, Schreiner GF, Johnson RJ . Role of the microvascular endothelium in progressive renal disease[J]. J Am Soc Nephrol, 2002,13(3):806-816
[4]	Burns KD . Angiotensin Ⅱand its receptors in the diabetic kidney[J]. Am J Kidney Dis, 2000,36(3):449-467 doi: 10.1053/ajkd.2000.16192
[5]	He XJ, Yi ZW, He QN, Dang XQ, Wu XC, Lu XY, Fang JH . Effects of catechin on expressions of ET and NO in rats with nephrotic syndrome[J]. Zhonghua Er Ke Za Zhi, 2002,40(9):550-554
	何小解, 易著文, 何庆南, 党西强, 吴小川, 卢向阳, 方建虎 . 儿茶素对肾病大鼠血浆及肾皮质内皮素、一氧化氮表达影响的研究[J]. 中华儿科杂志, 2002,40(9):550-554
[6]	Yang F, de Villiers WJ, McClain CJ, Varilek GW . Green tea polyphenols block endotoxin-induced tumor necrosis factor-production and lethality in a murine model[J]. J Nutr, 1998,128(12):2334-2340 doi: 10.1093/jn/128.12.2334
[7]	Zhao ZJ, Zhang XX, Shi SZ, Zhang Y, Chen XM, Ye CH, Niu JZ . The mechanism of delaying rat chronic renal failure development by Shenkang injection[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2000,6(10):16-20
	赵宗江, 张新雪, 师锁柱, 张颖, 陈香美, 叶传蕙, 牛建昭 . 肾康注射液延缓5/6肾切除大鼠慢性肾功能衰竭进展的机理探讨[J]. 中国中医基础医学杂志, 2000,6(10):16-20
[8]	Raij L, Azar S, Keane W . Mesangial immune injury, hypertension, and progressive glomerular damage in Dahl rats[J]. Kidney Int, 1984,26(2):137-143 doi: 10.1038/ki.1984.147
[9]	Radford MG Jr, Donadio JV Jr, Bergstralh EJ, Grande JP . Predicting renal outcome in IgA nephropathy[J]. J Am Soc Nephrol, 1997,8(2):199-207
[10]	Kang DH, Joly AH, Oh SW, Hugo C, Kerjaschki D, Gordon KL, Mazzali M, Jefferson JA, Hughes J, Madsen KM, Schreiner GF, Johnson RJ . Impaired angiogenesis in the remnant kidney model: I. Potential role of vascular endothelial growth factor and thrombospondin-1[J]. J Am Soc Nephrol, 2001,12(7):1434-1447
[11]	Shimizu A, Kitamura H, Masuda Y, Ishizaki M, Sugisaki Y, Yamanaka N . Rare glomerular capillary regeneration and subsequent capillary regression with endothelial cell apoptosis in progressive glomerulonep-hritis[J]. Am J Pathol, 1997,151(5):1231-1239
[12]	Kang DH, Johnson RJ . Vascular endothelial growth factor: a new player in the pathogenesis of renal fibrosis[J]. Curr Opin Nephrol Hypertens, 2003,12(1):43-49 doi: 10.1097/00041552-200301000-00008
[13]	Navar LG, Nishiyama A . Why are angiotensin concentrations so high in the kidney?[J]. Curr Opin Nephrol Hypertens, 2004,13(1):107-115 doi: 10.1097/00041552-200401000-00015
[14]	Crowley SD, Gurley SB, Oliverio MI, Pazmino AK, Griffiths R, Flannery PJ, Spurney RF, Kim HS, Smithies O, Le TH, Coffman TM . Distinct roles for the kidney and systemic tissues in blood pressure regulation by the renin-angiotensin system[J]. J Clin Invest, 2005,115(4):1092-1099 doi: 10.1172/JCI23378
[15]	刘国树 . 高血压病防治[M]. 北京: 金盾出版社,2000: 94-95
	Liu GS. Prevention and treatment of hypertension disease[M]. Beijing: Jindun Publishing House,2000: 94-95
[16]	Kaschina E, Unger T. Angiotensin AT1/AT2 receptors: regulation, signalling and function[J].Blood Press, 2003,12(2):70-88 doi: 10.1080/08037050310001057
[17]	Liu Y, Sun YF, Ma GX, Li J, Meng G, Han L, Liu X, Li MG . Correlating endothelin releasing function of vascular endothelial cells to tea polyphenols and angiotensinⅡ.[J]. Zhonguo Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu, 2008,12(2):381-384
	刘玉, 孙运峰, 马贵喜, 李靖, 蒙果, 韩磊, 刘昕, 李鸣皋 . 血管内皮细胞内皮素分泌功能与茶多酚及血管紧张素Ⅱ的相关性[J]. 中国组织工程研究与临床康复, 2008,12(2):381-384

Related Articles 15

[1]	Rakhi Chakraborty, Swarnendu Roy. Angiotensin-converting enzyme inhibitors from plants: A review of their diversity, modes of action, prospects, and concerns in the management of diabetes-centric complications. Journal of Integrative Medicine, 2021, 19(6): 478-492.
[2]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[3]	Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[4]	Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[5]	Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[6]	Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[7]	Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[8]	Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[9]	Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[10]	Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[11]	Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[12]	Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[13]	Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[14]	Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[15]	Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng . Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2]	Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3]	Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4]	Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5]	Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6]	Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7]	Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8]	Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9]	Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10]	Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278

Group	n	GSI		TIS
Group	n	8 weeks	12 weeks	8 weeks	12 weeks
Sham-operated	10	6.24±0.96	11.75±0.49	0.14±0.02	0.19±0.04
Untreated	10	73.56±4.38**	114.72±5.91**^▲▲	3.78±0.17**	5.63±0.25**^▲▲
Catechin	10	51.07±3.75**^△△	79.87±4.33**^△▲▲	2.04±0.12**^△△	3.76±0.47**^△▲

Group	n		Renal glomerulus	Renal tubule
Group	n	8 weeks	12 weeks	8 weeks	12 weeks
Sham-operated	10	46.32±2.90	48.84±3.77	700.14±36.02	692.21±25.66
Untreated	10	25.38±1.82**	14.26±1.01**^▲▲	403.78±10.12**	335.23±15.25**^▲▲
Catechin	10	36.28±3.62**^△△	24.96±1.18**^△△▲▲	512.80±16.12**^△△	468.76±11.28**^△△▲

Group	n	ACE (U/L)		Ang Ⅱ(ng/L)
Group	n	8 weeks	12 weeks	8 weeks	12 weeks
Sham-operated	10	38.25±2.58	42.32±3.09	348.8±13.7	359.6±15.2
Untreated	10	96.54±5.17**	113.56±9.36**^▲▲	764.8±31.2**	868.6±43.5**^▲
Catechin	10	68.27±4.63**^△△	85.32±6.95**^△△▲▲	579.8±28.7**^△△	648.8±23.6**^△△▲

Group	n	ACE (U/mg)		Ang Ⅱ(pg/mg)
Group	n	8 weeks	12 weeks	8 weeks	12 weeks
Sham-operated	10	41.95±3.77	45.84±3.68	208.5±11.2	216.8±9.9
Untreated	10	148.89±8.66**	176.58±7.78**	578.8±23.6**	748.8±35.6**^▲
Catechin	10	89.54±4.70**^△△	108.68±7.78**^△△	411.4±20.7**^△△	446.8±23.0**^△△

Protective effect of catechin on renal microvessels in 5/6 nephrectomized rats and its mechanism

RichHTML

PDF (PC)

Like