Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 535-540.doi: 10.3736/jcim20100605

• Original Experimental Research • Previous Articles     Next Articles

Antidiabetic effects of Tangnaikang on obese Zucker rats and the mechanism

Xiang-yu Guoa,b,Ying Duanc,Juan-e Lia,b,Li-xia Yanga,Lian-sha Huanga,b,Zhi-cheng Wanga,He-yao Wangd,Tong-hua Liua,b   

  1. a Beijing University of Chinese Medicine, Beijing 100029, China
    b Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
    c Department of Traditional Chinese Medicine, Beijing Tongren Hospital, Beijing 100730, China
    d The Third Laboratory of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • Received:2010-01-08 Accepted:2010-04-20 Online:2010-06-20 Published:2010-06-15

Objective

To observe the effects of Tangnaikang (TNK), a compound traditional Chinese herbal medicine, on glucose metabolism and insulin resistance in obese Zucker rats.
Methods

Twelve male obese Zucker rats, 6 weeks old, were randomly divided into control group and TNK group (3.24 g/kg) after being fed for 2 weeks. All rats received high-fat diet and 4-week treatment. Body weight and blood glucose were tested every week. Oral glucose tolerance test (OGTT) was performed and fasting insulin level was tested on days 0, 14 and 28. Triglyceride, cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and free fatty acids (FFA) were tested on day 28. Glucose infusion rate (GIR) was tested by hyperinsulinemic-euglycemic clamp from day 29. The protein expressions of protein kinase B (Akt), phospho-Akt (p-Akt) (Thr308) and glucose transporter protein 4 (GLUT4) in skeletal muscle and GLUT4 in adipose tissue were measured after hyperinsulinemic-euglycemic clamp test.
Results

Compared with the control group, the fed blood glucose level and glucose level of OGTT at 120 min had a significant decline in TNK group on day 28, and TNK caused no alteration of the fasting serum insulin, and the GIR increased significantly in hyperinsulinemic-euglycemic clamp study. Furthermore, TNK increased Akt and p-Akt (Thr308) protein expressions in skeletal muscle and decreased the protein expression of GLUT4 in white adipose tissue. Body weight, and triglyceride, cholesterol, LDL-C and FFA contents were slightly decreased in the TNK group, but there were no statistically significant effects.
Conclusion

TNK increases the protein expressions of Akt and p-Akt (Thr308) of the signal transduction pathway to influence the translocation of GLUT4 in skeletal muscle and improves glucose metabolism by reducing insulin resistance.

Key words: Compound (TCD), Diabetes mellitus, Type 2, Insulin resistance, Glucose transporter protein 4, Glucose clamp technique, Obese Zucker rats

"

"

"

"

"

"

Figure 1

Expressions of Akt, phospho-Akt (Thr308) and GLUT4 proteins in skeletal muscle and expression of GLUT4 protein in white adipose tissue detected by Western blotting"

[1] Centers for Disease Control and Prevention. National diabetes fact sheet: general information and national estimates on diabetes in the United States. 2005.[ 2010-04-16]. .
[2] Giugliano D, Ceriello A, Esposito K . Glucose metabolism and hyperglycemia[J]. Am J Clin Nutr, 2008,87(1):217S-222S
doi: 10.1093/ajcn/87.1.217S
[3] Expert Committee on the Diagnosis and Classification of Diabetes Mellitus . Report of the expert committee on the diagnosis and classification of diabetes mellitus[J]. Diabetes Care, 2003,26(Suppl 1):S5-S20
doi: 10.2337/diacare.26.2007.S5
[4] Coutionho M, Gerstein HC, Wang Y, Yusuf S . The relationship between glucose and incident cardiovascular events. A metaregression analysis of published data from 20 studies of 95 783 individuals followed for 12.4 years[J]. Diabetes Care, 1999,22(2):233-240
doi: 10.2337/diacare.22.2.233
[5] DECODE Study Group , European Diabetes Epidemiology Group.Is the current definition for diabetes relevant to mortality risk from all causes and cardiovascular and noncardiovascular diseases?[J]. Diabetes Care, 2003,26(3):688-696
doi: 10.2337/diacare.26.3.688
[6] Li WL, Zheng HC, Bukuru J, De Kimpe N . Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus[J]. J Ethnopharmacol, 2004,92(1):1-21
doi: 10.1016/j.jep.2003.12.031
[7] Pénicaud L, Ferré P, Terretaz J, Kinebanyan MF, Leturque A, Doré E, Girard J, Jeanrenaud B, Picon L . Development of obesity in Zucker rats. Early insulin resistance in muscles but normal sensitivity in white adipose tissue[J]. Diabetes, 1987,36(5):626-631
doi: 10.2337/diab.36.5.626
[8] Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS, Yuan CS . Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component[J]. Diabetes, 2002,51(6):1851-1858
doi: 10.2337/diabetes.51.6.1851
[9] Kraeft S, Schwarzer K, Eiden S, Nuesslein-Hildesheim B, Preibisch G, Schmidt I . Leptin responsiveness and gene dosage for leptin receptor mutation(fa) in newborn rats[J]. Am J Physiol, 1999,276(5 Pt 1):E836-E842
[10] Frde TS, Medeiros YS . Animal models to test drugs with potential antidiabetic activity[J]. J Ethnopharmacol, 2008,115(2):173-183
doi: 10.1016/j.jep.2007.10.038
[11] Cline GW, Petersen KF, Krssak M, Shen J, Hundal RS, Trajanoski Z, Inzucchi S, Dresner A, Rothman DL, Shulman GI . Impaired glucose transport as a cause of decreased insulin-stimulated muscle glycogen synjournal in type 2 diabetes[J]. N Engl J Med, 1999,341(4):240-246
doi: 10.1056/NEJM199907223410404
[12] Shepherd PR, Kahn BB . Glucose transporters and insulin action-implications for insulin resistance and diabetes mellitus[J]. N Engl J Med, 1999,341(4):248-257
doi: 10.1056/NEJM199907223410406
[13] Farese RV, Sajan MP, Standaert ML . Insulin-sensitive protein kinases(atypical protein kinase C and protein kinase B/Akt): actions and defects in obesity and type Ⅱ diabetes[J]. Exp Biol Med (Maywood), 2005,230(9):593-605
doi: 10.1177/153537020523000901
[14] Abel ED, Peroni O, Kim JK, Kim YB, Boss O, Hadro E, Minnemann T, Shulman GI, Kahn BB . Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver[J]. Nature, 2001,409(6821):729-733
doi: 10.1038/35055575
[15] Crist GH, Xu B, Lanoue KF, Lang CH . Tissue-specific effects of in vivo adenosine receptor blockade on glucose uptake in Zucker rats[J]. FASEB J, 1998,12(13):1301-1308
doi: 10.1096/fasebj.12.13.1301
[16] Pederson D, Kahn R, Kahn BB . Divergent regulation of the GLUT1 and GLUT4 glucose transporters in isolated adipocytes from Zucker rats[J]. J Clin Invest, 1992,89(6):1964-1973
doi: 10.1172/JCI115804
[17] Carvalho E, Kotani K, Peroni OD, Kahn BB . Adipose-specific overexpression of GLUT4 reverses insulin resistance and diabetes in mice lacking GLUT4 selectively in muscle[J]. Am J Physiol Endocrinol Metab, 2005,289(4):E551-E561
doi: 10.1152/ajpendo.00116.2005
[18] Kruszynska YT, Olefsky JM . Cellular and molecular mechanisms of noninsulin dependent diabetes mellitus[J]. J Investig Med, 1996,44(8):413-248
[1] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[2] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[3] Mehranghiz Ebrahimi-Mameghani, Mohammad Asghari-Jafarabadi, Khatereh Rezazadeh. TCF7L2-rs7903146 polymorphism modulates the effect of artichoke leaf extract supplementation on insulin resistance in metabolic syndrome: A randomized, double-blind, placebo-controlled trial. Journal of Integrative Medicine, 2018, 16(5): 329-334.
[4] Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[5] Chun-song Hu, Tengiz Tkebuchava. SEEDi1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[6] Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[7] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[8] Yee Chi Peggy Wong. Need of integrated dietary therapy for persons with diabetes mellitus and “unhealthy” body constitution presentations. Journal of Integrative Medicine, 2016, 14(4): 255-268.
[9] Ausanee Wanchai, Duangjai Phrompayak. Use of complementary and alternative medicine among Thai patients with type 2 diabetes mellitus. Journal of Integrative Medicine, 2016, 14(4): 297-305.
[10] Sulaiman Al-Eidi, SalwaTayel, Fatima Al-Slail, Naseem Akhtar Qureshi, Imen Sohaibani, Mohamed Khalil, Abdullah Mohammad Al-Bedah. Knowledge, attitude and practice of patients with type 2 diabetes mellitus towards complementary and alternative medicine. Journal of Integrative Medicine, 2016, 14(3): 187-196.
[11] Mohammad Hashem Hashempur, Mojtaba Heydari, Seyed Hamdollah Mosavat, Seyyed Taghi Heydari, Mesbah Shams. Complementary and alternative medicine use in Iranian patients with diabetes mellitus. Journal of Integrative Medicine, 2015, 13(5): 319-325.
[12] Ahmad Ghorbani. Clinical and experimental studies on polyherbal formulations for diabetes: Current status and future prospective. Journal of Integrative Medicine, 2014, 12(4): 336-345.
[13] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[14] Rajadurai Akilen​, Zeller Pimlott​, Amalia Tsiami​, Nicola Robinson​. The use of complementary and alternative medicine by individuals with features of metabolic syndrome. Journal of Integrative Medicine, 2014, 12(3): 171-174.
[15] Hai-li Jiang, Jing-jing Ni, Wei-fei Zhang, Wen-jin Huang​, Ming-yue Zhou​, Wen-jun Sha​, Jun-yan Li​, Fu-feng Li​, Zhu Ting​​, Xin Xia, Jun Zhang​, Yuan-dong Shen​, Li-gang Zhou​. The role of central nervous system on hypoglycemia and the feasibility of the brain theory in traditional Chinese medicine on treatment of diabetes mellitus. Journal of Integrative Medicine, 2014, 12(1): 1-6.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[4] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[5] Nian-hong Wang , Jun-tao Yan , Wu-quan Sun , Yong-shan Hu , Jun Xia , Li-cheng Wei , Jie Jia , Gui-lin Ouyang , Yong He , Yan-ming Guo , Jie Xu . Effects of early application of Tuina treatment on quadriceps surface myoelectricity in patients after total knee arthroplasty: a randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(11): 1247-1253
[6] Dong-tao Li, Jian Wang , Hong-yang Jiang , Feng-lei Shi , Fu-yu Li , Ji-hong Liu , Yong-mei Cheng , Nan Yan , Ai-hua Hu, Mei-zeng Zhang , Jie Li, Ling-bo Wei , Rong-qin Jiang. Quantitative evaluation of the degrees of traditional Chinese medicine qualitative syndromes of osteoporosis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1254-1262
[7] Veena Sharma , Pracheta Janmeda , Ritu Paliwal , Shatruhan Sharma. Antihepatotoxic activity of Euphorbia neriifolia extract against N-nitrosodiethylamine-induced hepatocarcinogenesis in mice. Journal of Chinese Integrative Medicine, 2012, 10(11): 1303-1309
[8] Peng-fei Gao, Kenji Watanabe. Introduction of the World Health Organization project of the International Classification of Traditional Medicine. Journal of Chinese Integrative Medicine, 2011, 9(11): 1161-1164
[9] Oluwole Busayo Akinola. Should complementary and alternative medicine familiarisation modules be taught in African medical schools?. Journal of Chinese Integrative Medicine, 2011, 9(11): 1165-1169
[10] Kishan Lal Tiwari, Shailesh Kumar Jadhav, Veenu Joshi. An updated review on medicinal herb genus Spilanthes. Journal of Chinese Integrative Medicine, 2011, 9(11): 1170-1178