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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (10): 968-973.doi: 10.3736/jcim20101009

• Original Experimental Research • Previous Articles     Next Articles

Chinese herbal medicine Jianpi Jiedu Formula down-regulates the expression of vascular endothelial growth factor in human gastric cell line MKN45 induced by Helicobacter pylori by inhibiting cyclooxygenase-2

NN Liu,N Zhou,Y Wang,X Liu,LH Zhou,Q Wu,J Sun,ZZ Fan,Q Li   

  1. Department of Oncology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
  • Received:2010-03-19 Accepted:2010-06-30 Online:2010-10-20 Published:2010-10-15

Objective

To investigate the effects of Jianpi Jiedu Formula (JPJDF), a compound Chinese herbal medicine, on vascular endothelial growth factor (VEGF) expression induced by Helicobacter pylori (Hp) in human gastric cancer cells, and to explore the possible mechanism.
Methods

Human gastric MKN45 cancer cells were infected with standard Hp NCTC11637 by coculture, and the cells were divided into 7 groups: normal control group, Hp group, NS398 inhibitor group, blank serum group, and 5%, 10% and 20% JPJDF groups. The expressions of VEGF and cyclooxygenase-2 (COX-2) mRNAs in human gastric cancer cells infected by Hp were evaluated by real-time fluorogenic quantitative polymerase chain reaction (PCR). After inhibiting the expression of COX-2 with COX-2 specific inhibitor NS398 (50 μmol/L), VEGF mRNA expression induced by Hp in human gastric cancer MKN45 cells was evaluated by real-time fluorogenic quantitative PCR.
Results

Real-time fluorogenic quantitative PCR results showed that COX-2 mRNA expression increased significantly after MKN45 cells were infected with Hp for 6 h (P<0.01), and VEGF mRNA expression also increased significantly after MKN45 cells were infected with Hp for 12 h as compared with the normal control group (P<0.01). After inhibiting COX-2 expression with COX-2 specific inhibitor NS398, Hp-induced VEGF mRNA expression significantly reduced (P<0.01). 5%, 10% and 20% JPJDF-containing sera all down-regulated VEGF and COX-2 mRNA expressions induced by Hp in a dose-dependent manner as compared with the Hp infection group.
Conclusion

Hp could induce the expressions of COX-2 and VEGF in human gastric cancer cells, and JPJDF down-regulates Hp-induced expression of VEGF by inhibiting COX-2, which might be one of the important mechanisms of its prevention and treatment of gastric cancer.

Key words: Helicobacter pylori, Jianpi Jiedu Formula, Stomach neoplasms, Cyclooxygenase-2, Vascular endothelial growth factor

Table 1

Primer and probe oligonucleotide sequences of COX-2, VEGF and GAPDH for real-time fluorogenic quantitative PCR"

Figure 1

Effects of Jianpi Jiedu Formula on Hp-induced VEGF mRNA expression in MKN45 cells A: Normal control group; B: Hp 12 h group; C: Blank serum group; D: 5% JPJDF serum group; E: 10% JPJDF serum group; F: 20% JPJDF serum group. **P<0.01, vs normal control group; ΔP<0.05, ΔΔP<0.01, vs Hp 12 h group; ▲▲P<0.01, vs 5% JPJDF serum group."

Figure 2

Effects of Jianpi Jiedu Formula on Hp-induced COX-2 mRNA expression in MKN45 cells A: Normal control group; B: Hp 6 h group, C: Blank serum group; D: 5% JPJDF serum group; E: 10% JPJDF serum group; F: 20% JPJDF serum group. **P<0.01, vs normal control group; ΔΔP<0.01, vs Hp 6 h group; ▲▲P<0.01, vs 5% JPJDF serum group."

Figure 3

Effects of COX-2 on Hp-induced VEGF mRNA expression in MKN45 cells A: Normal control group; B: MKN45+NS398 group; C:Hp 12h group, D: NS398 inhibitor group. **P<0.01, vs normal control group; △△P<0.01, vs Hp 12 h group."

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