高效液相色谱法测定巴豆中巴豆苷的含量

doi:10.3736/jcim20091107

Abstract

Objective

To develop a high-performance liquid chromatographic method for the determination of isoguanosine in Semen Crotonis Tiglii.
Methods

The determination was done by using reverse-phase high-performance liquid chromatography with a Hedera ODS-2 column (4.6 mm ×250 mm, 5 μm). Elution was employed with the mobile phase of acetonitrile-methanol-water (1︰4︰95) at flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm and the column temperature was 25 ℃. Injection volume was 10 μL.
Results

The linear range of isoguanosine was from 0.161 to 0.967 mg. The correlation coefficient of the calibration curves was 0.999 8. The average recovery rate was 98.78% with relative standard deviation of 0.02%. Conclusion: The results show that the method is simple, accurate, and repeatable, and it is suitable for quality control of Semen Crotonis Tiglii.

Key words: High-performance liquid chromatography, Semen Crotonis Tiglii, Isoguanosine

Yun Ling , Hong-yu Zhao, Xin-liang Pan, Chun-xiang Zhou. Determination of isoguanosine in Semen Crotonis Tiglii by high-performance liquid chromatography. Journal of Chinese Integrative Medicine, 2009, 7(11): 1057-1060.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20091107

http://www.jcimjournal.com/EN/Y2009/V7/I11/1057

TrendMD

Figures/Tables 3

Table 1

Table 2

Figure 1

References 11

[1]	China Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China(Part one).(2005 ed)[M]. Beijing: Chemical Industry Press, 2005,( 2005 ed):55
	国家药典委员会. 中华人民共和国药典(一部)(2005年版)[M]. 北京: 化学工业出版社, 2005,( 2005年版):55
[2]	Zhang JX, Wang Y . The contrast experiment on crude and processed Semen Crotonis Tiglii[J]. Zhong Yao Cai, 1992,15(9):29-30
	张静修, 王毅 . 生, 熟巴豆对比实验[J]. 中药材, 1992,15(9):29-30
[3]	Li JR, Zhu J, Gao XS . Effects of compatibility with Croton tiglium on decanta efficacy of Radix et Rhizoma Rhei[J]. Zhong Cheng Yao, 1993,15(5):26-27
	李建荣, 朱江, 高晓山 . 配伍对大黄致泻作用的影响(Ⅱ)——大黄与巴豆配伍[J]. 中成药, 1993,15(5):26-27
[4]	Zhao M, Zhao QZ, Zhang CY, Hou C, Guo JL, Wang P, Liu LX, Yao HY, Yu SY . Effects of carcinogen DEN and tumor promotor croton oil on rat liver alpha1-inhibitor 3 gene expression in vivo[J]. Sheng Wu Hua Xue Za Zhi, 1992,8(6):730-734
	赵玫, 赵清正, 张春燕, 侯充, 郭金利, 王萍, 刘立新, 姚红芸, 于树玉 . 致癌剂DEN、促癌剂巴豆油对大鼠肝α1抑制因子3基因表达的影响[J]. 生物化学杂志, 1992,8(6):730-734
[5]	Sun SS, Zhao YJ, Yuan SQ . Effects of Badoushuang(BDS) on anti-inflammation, immune function, analgesic action and mutation[J]. Zhong Yao Yao Li Yu Lin Chuang, 1993,9(3):36-38
	孙颂三, 赵燕洁, 袁士琴 . 巴豆霜对抗炎、免疫、镇痛及致突变的影响[J]. 中药药理与临床, 1993,9(3):36-38
[6]	Liu XD, Sui ZY . Effects of Croton tiglium total alkaloids on cancer cell cytoplasm membrane fluidity and structure of the cytoplasmic matrix[J]. Shandong Zhong Yi Xue Yuan Xue Bao, 1995,19(3):192-194
	刘秀德, 隋在云 . 巴豆总生物碱对癌细胞质膜流动性及胞浆基质结构的影响[J]. 山东中医学院学报, 1995,19(3):192-194
[7]	Tian YW . Differentiation-inducing effect of the Croton tiglium aquatic extracts to HL-60 cells[J]. Shanxi Yi Yao Za Zhi, 2002,31(3):265 doi: 10.3969/j.issn.0253-9926.2002.03.061
	田艳伟 . 巴豆水提液对HL-60细胞的诱导分化作用[J]. 山西医药杂志, 2002,31(3):265 doi: 10.3969/j.issn.0253-9926.2002.03.061
[8]	Xu JG, Ma JY, Yang GS, Ren LS, Guo YD, Zhang HX . Study on differentiation-inducing effects of Croton tiglium decoction on human promyelocytes[J]. Zhonghua Xue Ye Xue Za Zhi, 1990,11(10):538-539
	徐建国, 马俊英, 杨贵生, 任连生, 郭亚东, 张鸿翔 . 巴豆煎液对人早幼粒细胞白血病细胞的诱导分化研究[J]. 中华血液学杂志, 1990,11(10):538-539
[9]	Hu J, Gao WY, Ling NS, Shi SX, Wang WY, Zhang TJ . Volatility elements of Croton tiglium and Crotonis pulveratum analyzed by GC-MS[J]. Zhongguo Zhong Yao Za Zhi, 2008,33(4):464-465 doi: 10.3321/j.issn:1001-5302.2008.04.037
	胡静, 高文远, 凌宁生, 石世学, 王文燕, 张铁军 . 巴豆和巴豆霜挥发性成分的GC-MS分析[J]. 中国中药杂志, 2008,33(4):464-465 doi: 10.3321/j.issn:1001-5302.2008.04.037
[10]	Kim JH, Lee SJ, Han YB, Moon JJ, Kim JB . Isolation of isoguanosine from Croton tiglium and its antitumor activity[J]. Arch Pharm Res, 1994,17(2):115-118 doi: 10.1007/BF02974234 pmid: 10319142
[11]	Tang GY, Chen MH, Wu HJ . Isolation and determination of crotin toxin by high performance gel filtration chromatography(GFC) with photodiode array detector[J]. Se Pu, 1994,12(4):244-246
	唐根源, 陈明晃, 吴红京 . 高效凝胶过滤色谱法(光电二极管阵列检测器)分离、测定巴豆毒蛋白[J]. 色谱, 1994,12(4):244-246

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2]	Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3]	Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4]	Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5]	Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6]	Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7]	Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8]	Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9]	Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10]	Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278

Semen Crotonis Tiglii (g)	Assayed isoguanosine (mg)	Added reference substance amount (mg)	Detected amount (mg)	Recovery rate (%)	Average recovery rate	RSD
0.3	1.613 2	1.612	3.198 6	98.35
0.3	1.619 7	1.612	3.176 3	96.56
0.3	1.615 4	1.612	3.258 8	101.95	98.78%	0.02%
0.3	1.617 5	1.612	3.228 4	99.93
0.3	1.615 4	1.612	3.163 7	96.05
0.3	1.613 2	1.612	3.222 7	99.84

No.	Habitat	n	Batch No.	Concentration
1	Yunnan	3	20080906	12.0±1.4
2	Yunnan	3	20081145	11.9±1.8
3	Yunnan	3	20081028	16.2±0.5
4	Sichuan	3	080301	13.4±1.4
5	Guangxi	3	080815	10.8±1.8
6	Sichuan	3	080816	11.2±1.5
7	Sichuan	3	080817	11.7±0.4
8	Sichuan	3	081206	12.4±1.8
9	Yunnan	3	090205	10.2±0.6
10	Gansu	3	090210	10.6±1.7

Determination of isoguanosine in Semen Crotonis Tiglii by high-performance liquid chromatography

RichHTML

PDF (PC)

Cited

Like

Abstract

Cite this article

Share this article