Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (10): 1140-1148.doi: 10.3736/jcim20121011

Previous Articles     Next Articles

Effects of Chinese herbal medicine Shenxiong Yujing Granule on regeneration of neural cells in rats with diabetes-associated cerebral ischemia

Hui Han1,2, Li-min Wu3, Ming-xiang Han2(), Wen-ming Yang1, Dao-jun Xie1, Zhao-hui Fang4, Dan-chen Chu1, Fang Fang1   

  1. 1. Department of Neurology, the First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China
    2. Famous Experts in Traditional Chinese Medicine Workroom of State Administration of Traditional Chinese Medicine, the First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China
    3. Medical Research Center, Anhui Provincial Hospital, Hefei 230001, Anhui Province, China
    4. Department of Endocrinology, the First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui Province, China
  • Received:2012-03-30 Accepted:2012-04-20 Online:2012-10-20 Published:2018-10-15
  • Contact: Han Ming-xiang E-mail:hmx401125@sina.com

Objective: To establish a rat model of diabetes-associated cerebral ischemia due to qi and yin deficiency and blood stasis, and to investigate the effects of Radix Ginseng, Rhizoma Chuanxiong, Rhizoma Polygonati Odoratiand Rhizoma Polygonati Sibirici Granule (Shenxiong Yujing Granule), which has the function of strengthening qi, nourishing yin, and activating blood, on proliferation, differentiation and survival of neural cells in rats with diabetes-associated cerebral ischemia. .

Methods: Rats were divided into sham-operation, diabetes plus ischemia reperfusion injury model, Shenxiong Yujing Granule and Radix Ginseng and Rhizoma Chuanxiong Granule (Shenxiong Granule) groups with 20 rats in each. The 5-bromo-2′-deoxyuridine (BrdU) incorporation assay and immunohistochemical method were used to investigate the proliferation, differentiation and survival of neural cells in dentate gyrus of rats with diabetes-associated cerebral ischemia.

Results: The number of newly proliferating cells in subgranular zone of dentate gyrus was increased in the model group, but there was no significant difference compared with 7 day treatment with Shenxiong Yujing Granule. Shenxiong Yujing Granule significantly increased the survival rate and promoted the differentiation of newly proliferating neurons after 21-day treatment (P<0.01). In addition, the beneficial effect of Shenxiong Yujing Granule was considerably greater than that of the Shenxiong Granule (P<0.01).

Conclusion: Shenxiong Yujing Granule can increase the survival rate and promote the differentiation of newly proliferating neurons in rats with diabetes-associated cerebral ischemia of dual deficiency of qi and yin and blood stasis obstructing the collaterals. The effect is greater than that of Shenxiong Granule.

Key words: drugs, Chinese herbal, diabetes mellitus, brain ischemia, nerve regeneration, neurons, disease models, animal, astrocytes, rats

Figure 1

Effects of Shenxiong Yujing Granule on neurocyte proliferation in dentate gyrus of hippocampus in rats with diabetes and ischemia reperfusion injury (immunohistochemical method, fluorescence microscopy) Shenxiong Yujing Granule is composed of Radix Ginseng, Rhizoma Chuanxiong, Rhizoma Polygonati Odorati and Rhizoma Polygonati Sibirici Granule and Shenxiong Granule is composed of Radix Ginseng and Rhizoma Chuanxiong. The sections were labeled with BrdU antibody (blue, nuclear staining). A to D showed BrdU-positive neurocytes on day 7. E to H showed BrdU-positive neurocytes on day 21. A and E: Sham-operation group; B and F, Diabetes plus ischemia reperfusion injury group; C and G: Shenxiong Yujing Granule group; D and H: Shenxiong Granule group. The black arrows in pictures point to the BrdU-labeled cells. Bar: 50 μm; BrdU: 5-bromo-2′-deoxyuridine."

Figure 2

Quantitative analysis of the effects of Shenxiong Yujing Granule on neurocyte survival and proliferation in dentate gyrus of hippocampus in rats with diabetes and ischemia reperfusion injuryShenxiong Yujing Granule is composed of Radix Ginseng, Rhizoma Chuanxiong, Rhizoma Polygonati Odorati and Rhizoma Polygonati Sibirici Granule and Shenxiong Granule is composed of Radix Ginseng and Rhizoma Chuanxiong. A: BrdU-positive neurocytes on the 7th and 21st days after cerebral ischemia; B: Ratio of BrdU-positive neurocytes on the 7th and 21st days, namely the ratio of new regenerative and surviving cells. **P<0.01, vs sham-operation group; △P<0.05, △△P<0.01, vs diabetes plus ischemia reperfusion injury group; ▲P<0.05, vs Shenxiong Granule group."

Figure 3

Effects of Shenxiong Yujing Granule on neurocyte and astrocyte differentiation in dentate gyrus of hippocampus in rats with diabetes and ischemia reperfusion injury on day 21 (immunofluorescence method, fluorescence microscopy) The sections were isolated from a rat in the Shenxiong Yujing Granule group and were double-labeled with NeuN (A, green, nuclear staining) and BrdU (B, red, nuclear staining) or with GFAP (D, green, cytoplasmic staining) and BrdU (E, red, (blue, nuclear staining)) antibody. Some BrdU-positive cells colocalized with NeuN-positive cells (C, yellow); some BrdU-positive cells colocalized with GFAP-positive cells (F). The white arrows in pictures point to NeuN- or GFAP-BrdU double-labeled cells. Bar: 20 μm. The quantitative analysis of neuronal differentiation of new-born cells was shown in G. The percentage is recorded as the number of double-positive cells divided by that of BrdU-positive cells (G). BrdU: 5-bromo-2′-deoxyuridine; NeuN: neuron-specific nuclear protein; GFAP: glial fibrillary acidic protein. **P<0.01, vs sham-operation group; △△P<0.01, vs diabetes plus ischemia reperfusion injury group; ▲▲P<0.01, vs Shenxiong Granule group."

[1] Fang SL . New concept of diabetes prevention[J]. Shi Yong Zhong Yi Nei Ke Za Zhi, 2008,21(1):11-12
方水林 . 糖尿病防治新理念. 实用中医内科杂志. 2008,21(1):11-12.
[2] Zhang HY . Research on risk factors of type 2 diabetes mellitus complicated with cerebral infarction[J]. Di Si Jun Yi Da Xue Xue Bao, 2008,29(5):477
张海燕 . 2型糖尿病并发脑梗死的危险因素研究[J]. 第四军医大学学报, 2008,29(5):477
[3] Kaarisalo MM, Raiha I, Sivenius J, Immonen-Raiha P, Lehtonen A, Sarti C, Mahonen M, Torppa J, Tuomilehto J, Salomaa V . Diabetes worsens the outcome of acute ischemic stroke[J]. Diabetes Res Clin Pract, 2005,69(3):293-298
doi: 10.1016/j.diabres.2005.02.001 pmid: 16098927
[4] Han H, Tian JZ, Han MX, Yang WM, Fang ZH . Characteristics of traditional Chinese medicine syndromes in dabetic cerebral infarction[J]. Zhong Yi Yao Lin Chuang Za Zhi, 2010,22(11):1002-1005
韩辉, 田金洲, 韩明向, 杨文明, 方朝晖 . 糖尿病脑梗死的中医证候特征[J]. 中医药临床杂志, 2010,22(11):1002-1005
[5] Han H, Wang SY, Bao YC, Fang ZH, Yang WM, Han MX, Wang H, Wang XY, Zhang J, Wang MX, Dong T . Randomized controlled clinical study of supplementing qi, nourishing yin and activating blood circulation decoction in treating diabetes-associated cerebral infarction[J]. Zhong Yi Yao Lin Chuang Za Zhi, 2011,23(12):1052-1055
韩辉, 王守运, 鲍远程, 方朝晖, 杨文明, 韩明向, 汪瀚, 王晓旸, 张娟, 汪美霞, 董婷 . 益气养阴活血法拟方治疗糖尿病合并脑梗死随机对照临床研究[J]. 中医药临床杂志, 2011,23(12):1052-1055
[6] Salazar-Colocho P, Lanciego JL, Del Rio J, Frechilla D . Ischemia induces cell proliferation and neurogenesis in the gerbil hippocampus in response to neuronal death[J]. Neurosci Res, 2008,61(1):27-37
doi: 10.1016/j.neures.2008.01.008
[7] Qiao FX, Shen ZF, Ye F, Chen YT, Xie MZ . Streptozotocin-diabetic hamster: an animal model to evaluate hypoglycemics and hypolipidemics[J]. Zhongguo Tang Niao Bing Za Zhi, 2000,8(4):215-218
doi: 10.3321/j.issn:1006-6187.2000.04.007
乔凤霞, 申竹芳, 叶菲, 陈跃腾, 谢明智 . 链脲佐菌素糖尿病金黄地鼠模型建立的研究: 一种同时评价药物降血糖和调节血脂作用的动物模型[J]. 中国糖尿病杂志, 2000,8(4):215-218
doi: 10.3321/j.issn:1006-6187.2000.04.007
[8] Zhang YL, Zhang J, Hu XG, Li X, Zheng H, Jin XL . Establishment of rat model of ischemic stroke with qi deficiency and blood stasis syndrome and its evaluation system[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 2009,29(4):343-346
张允岭, 张锦, 扈新刚, 李秀, 郑宏, 金香兰 . 制作缺血性中风气虚血瘀证大鼠模型的实验研究[J]. 中国中西医结合杂志, 2009,29(4):343-346
[9] Fu XL, Fang ZQ . Modeling methods and appraisal of yin-deficiency syndrome in animals[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2004,18(2):51-54
付晓伶, 方肇勤 . 阴虚证动物模型的造模方法及评析[J]. 上海中医药大学学报, 2004,18(2):51-54
[10] Longa EZ, Weinstein PR, Carlson S, Cummins R . Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989,20(1):84-91
doi: 10.1161/01.STR.20.1.84
[11] Lang BT, Yan Y, Dempsey RJ, Vemuganti R . Impaired neurogenesis in adult type-2 diabetic rats[J]. Brain Res, 2009, 125825-33
doi: 10.1016/j.brainres.2008.12.026 pmid: 2649982
[12] Han H, Wu LM, Tu JW, Han MX . Studying the effect of Shenxiongqin injection in reducing the contents of ET-1, TNF-α, IL-1β of focal cerebral infarction in rats[J]. Anhui Yi Yao, 2005,9(1):17-18
韩辉, 吴丽敏, 涂晋文, 韩明向 . 人参川芎嗪注射液对局灶性大鼠脑梗死ET-1、TNF-α、IL-1β 含量的影响[J]. 安徽医药, 2005,9(1):17-18
[13] Han H, Wu LM, Wang H, Wang MX, Zhang J, Tu JW, Han MX . Effects of Renshen Chuanxiongqin Injection on SOD activity, MDA concentration in serum and EAA concentration in brain tissue in rats with focal cerebral ischemia[J]. Chengdu Zhong Yi Yao Da Xue Xue Bao, 2009,32(1):76-78, 90
韩辉, 吴丽敏, 汪瀚, 汪美霞, 张娟, 涂晋文, 韩明向 . 人参川芎嗪注射液对局灶性脑缺血大鼠血清SOD活性、MDA含量及脑组织氨基酸含量的影响[J]. 成都中医药大学学报, 2009,32(1):76-78, 90
[14] Han H, Wu LM, Yang WM, Wang MX, Dong T, Wang H, Han MX . Effect of Shenxiongqin Injection on hemorheology and cerebral water content in rats with focal cerebral ischemia[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2010,8(1):71-73
韩辉, 吴丽敏, 杨文明, 汪美霞, 董婷, 汪瀚, 韩明向 . 参芎嗪注射液对大鼠脑缺血损伤血液流变学、脑含水量的影响[J]. 中西医结合心脑血管病杂志, 2010,8(1):71-73
[15] Han H, Wu LM, Wang MX, Yang B, Wang H, Tu JW, Han MX . Effects of Renshen Chuanxiongqin Injection on t-PA concentration and its activity, PAI-1 concentration and histomorphological changes in rats with focal cerebral ischemia[J]. Anhui Zhong Yi Xue Yuan Xue Bao, 2008,27(6):37-40
韩辉, 吴丽敏, 汪美霞, 杨波, 汪瀚, 涂晋文, 韩明向 . 人参川芎嗪注射液对局灶性脑缺血大鼠血浆t-PA含量及活性、PAI-1含量、脑组织形态学的影响[J]. 安徽中医学院学报, 2008,27(6):37-40
[16] Han H, Wu LM, Xie Q, Dong T, Zhang J, Wang MX, Han MX . Protective effects of Ginseng Ligustrazine Injection against focal cerebral ischemia injury in rats[J]. Zhongguo Zhong Yi Ji Zheng, 2009,18(4):581-582, 602
韩辉, 吴丽敏, 解清, 董婷, 张娟, 汪美霞, 韩明向 . 人参川芎嗪注射液对大鼠局灶性脑缺血损伤的保护作用[J]. 中国中医急症, 2009,18(4):581-582, 602
[17] Ding DF, Xiang DX, Liu S, Jiang W . Extraction of polysaccharide from Polygonatum odoratum and its hypoglycemic effect on STZ induced diabetic rats[J]. Zhong Nan Yao Xue, 2005,3(4):222-224
丁登峰, 向大雄, 刘韶, 姜文 . 玉竹多糖的提取及其对链脲佐菌素诱导糖尿病大鼠血糖的影响[J]. 中南药学, 2005,3(4):222-224
[18] Xie YM, Yuwen Y, Wang YY . Review of secondary prevention with traditional Chinese medicine against ischemic stroke[J]. Shizhen Guo Yi Guo Yao, 2008,9(11):2730-2731
谢雁鸣, 宇文亚, 王永炎 . 缺血性中风的二级预防及中医药应用述评[J]. 时珍国医国药, 2008,9(11):2730-2731
[19] Chen Y , Sun XS . Research progress on pharmacologic actions of Rhizoma Polygonati Sibirici. Zhong Yao Xin Yao Yu Lin Chuang Yao Li. 2010; 21(3):328-330 Chinese.
陈晔, 孙晓生 . 黄精的药理研究进展[J]. 中药新药与临床药理, 2010,21(3):328-330
[20] Altman J, Das GD . Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats[J]. J Comp Neurol, 1965,124(3):319-335
doi: 10.1002/(ISSN)1096-9861
[21] Altman J, Das GD . Postnatal neurogenesis in the guinea-pig[J]. Nature, 1967,214(5193):1098-1101
doi: 10.1038/2141098a0 pmid: 6053066
[22] Eriksson PS, Perfilieva E, Bjork-Eriksson T, Alborn AM, Nordborg C, Peterson DA, Gage FH . Neurogenesis in the adult human hippocampus[J]. Nat Med, 1998,4(11):1313-1317
doi: 10.1038/3305 pmid: 9809557
Eriksson PS, Perfilieva E , Björk-Eriksson T , Alborn AM , Nordborg C ,Peterson DA , Gage FH .Neurogenesis in the adult human hippocampus[J]. Nat Med, 1998,4(11):1313-1317
[23] Gould E, Tanapat P , McEwen BS , Flügge G , Fuchs E .Proliferation of granule cell precursors in the dentate gyrus of adult monkeys is diminished by stress[J]. Proc Natl Acad Sci U S A, 1998,95(6):3168-3171
doi: 10.1073/pnas.95.6.3168
[24] Doetsch F, Caillé I, Lim DA, García-Verdugo JM, Alvarez-Buylla A . Subventricular zone astrocytes are neural stem cells in the adult mammalian brain[J]. Cell, 1999,97(6):703-716
doi: 10.1016/S0092-8674(00)80783-7
[25] Komitova M, Perfilieva E, Mattsson B, Eriksson PS, Johansson BB . Effects of cortical ischemia and postischemic environmental enrichment on hippocampal cell genesis and differentiation in the adult rat[J]. J Cereb Blood Flow Metab, 2002,22(7):852-860
doi: 10.1097/00004647-200207000-00010
[26] Pincus DW, Keyoung HM, Harrison-Restelli C, Goodman RR, Fraser RA, Edgar M, Sakakibara S, Okano H, Nedergaard M, Goldman SA . Fibroblast growth factor-2/brain-derived neurotrophic factor-associated maturation of new neurons generated from adult human subependymal cells[J]. Ann Neurol, 1998,43(5):576-585
doi: 10.1002/(ISSN)1531-8249
[27] Kempermann G, Kuhn HG, Gage FH . Genetic influence on neurogenesis in the dentate gyrus of adult mice[J]. Proc Natl Acad Sci U S A, 1997,94(19):10409-10414
doi: 10.1073/pnas.94.19.10409
[28] van Praag H, Christie BR, Sejnowski TJ, Gage FH . Running enhances neurogenesis, learning, and long-term potentiation in mice[J]. Proc Natl Acad Sci U S A, 1999,96(23):13427-13431
doi: 10.1073/pnas.96.23.13427
[29] Gould E, Beylin A, Tanapat P, Reeves A, Shors TJ . Learning enhances adult neurogenesis in the hippocampal formation[J]. Nat Neurosci, 1999,2(3):260-265
doi: 10.1038/6365 pmid: 10195219
[30] Gould E, Tanapat P . Lesion-induced proliferation of neuronal progenitors in the dentate gyrus of the adult rat[J]. Neuroscience, 1997,80(2):427-436
doi: 10.1016/S0306-4522(97)00127-9 pmid: 9284346
[31] Liu J, Solway K, Messing RO, Sharp FR . Increased neurogenesis in the dentate gyrus after transient global ischemia in gerbils[J]. J Neurosci, 1998,18(19):7768-7778
doi: 10.1523/JNEUROSCI.18-19-07768.1998
[32] Wiltrout C, Lang B, Yan Y, Dempsey RJ, Vemuganti R . Repairing brain after stroke: a review on post-ischemic neurogenesis[J]. Neurochem Int, 2007,50(7-8):1028-1041
doi: 10.1016/j.neuint.2007.04.011 pmid: 17531349
[1] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[2] Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[3] Yunita Sari, Akhyarul Anam, Annas Sumeru, Eman Sutrisna. The knowledge, attitude, practice and predictors of complementary and alternative medicine use among type 2 diabetes mellitus patients in Indonesia. Journal of Integrative Medicine, 2021, 19(4): 347-353.
[4] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[5] Pukar Khanal, Basanagouda M. Patil. Integration of network and experimental pharmacology to decipher the antidiabetic action of Duranta repens L.. Journal of Integrative Medicine, 2021, 19(1): 66-77.
[6] Baoyi Shao, Saiying Hou, Yuenyan Chan, Changchun Shao, Lixing Lao. Remission of new-onset type 2 diabetes mellitus in an adolescent using an integrative medicine approach: A case report. Journal of Integrative Medicine, 2021, 19(1): 85-88.
[7] Abdulhakim Abubakar, Abdullahi Balarabe Nazifi, Idris Mohammed Maje, Yusuf Tanko, Joseph Akpojo Anuka, Ezzeldin Mukthar Abdurahman. Antihyperglycaemic activity of ethylacetate extract of Chlorophytum alismifolium in type 2 diabetes: The involvement of peroxisome proliferator-activated receptor-γ and dipeptidyl peptidase-4. Journal of Integrative Medicine, 2021, 19(1): 78-84.
[8] Ming-sheng Lyu, De-ying Li, Shao-zhong Zhou, Cheng-jun Ban, Jun Yan. Adult-onset Still's disease successfully treated with Chinese herbal medicine: A case report with 15-month follow-up. Journal of Integrative Medicine, 2020, 18(6): 530-534.
[9] Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[10] Arthur Yin Fan, Sherman Gu, Sarah Faggert Alemi, Research Group for Evidence-based Chinese Medicine. Chinese herbal medicine for COVID-19: Current evidence with systematic review and meta-analysis. Journal of Integrative Medicine, 2020, 18(5): 385-394.
[11] Bin Lan, Jin-wen Ge, Shao-wu Cheng, Xi-long Zheng, Jun Liao, Chao He, Zheng-qing Rao, Guo-zuo Wang. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine, 2020, 18(4): 344-350.
[12] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[13] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[14] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[15] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228