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Journal of Chinese Integrative Medicine ›› 2013, Vol. 11 ›› Issue (6): 384-388.doi: 10.3736/jintegrmed2013049

• Research Article • Previous Articles     Next Articles

Effect of a novel dietary supplement on pH levels of healthy volunteers: A pilot study

Stephen Douglas Antona,b(), Xiaomin Luc, Ginny Bankd, Kacey Heekina, Debbie Sahaa, Pamela J. DubyakbHeather Ann Hausenblase,f   

  1. a. Department of Aging and Geriatric Research, University of Florida, Gainesville, Florida 32611, USA
    b. Department of Clinical and Health Psychology, University of Florida, Gainesville, Florida 32611, USA
    c. Department of Biostatistics, University of Florida, Gainesville, Florida 32610, USA
    d. RFI Ingredients, Blauvelt, New York 10913, USA
    e. Department of Sport and Exercise Sciences, University of Florida, Gainesville, Florida 32611, USA
    f. Department of Sport and Exercise Sciences, Jacksonville University, Jacksonville, Florida 32211, USA
  • Received:2013-06-05 Accepted:2013-07-24 Online:2013-11-10 Published:2013-11-15


To examine the effects of a greens alkalizing dietary supplement on urinary pH levels in individuals with lower-than-average pH levels.


The present study investigated the effects of an alkalizing formula (Reserveage Wholeganic GreensTM) on four individuals who had average urinary pH levels below 6.0 for three consecutive days. Following the three-day, baseline period, participants received Reserveage Wholeganic GreensTM for four consecutive days and were instructed to continue to measure their urine pH levels. Paired samples t-tests were used to examine pH levels before and after a four-day treatment period with Reserveage Wholeganic GreensTM.


Compared to baseline, mean urine pH levels in all volunteers were significantly higher following the supplementation with Reserveage Wholeganic GreensTM (5.89 ± 0.20 vs 5.56 ± 0.23; P<0.01). Participants’ pH levels were also significantly higher than baseline on days 5, 6, and 7 of the treatment period (P < 0.05). Noteworthy, on day 7, participants’ mean pH levels were significantly higher than at the beginning of the treatment period (6.03 ± 0.15 at day 7 vs 5.65 ± 0.24 at day 4; P < 0.01).


The findings of this study suggest that supplementation with Reserveage Wholeganic GreensTM has an alkalizing effect on the body and can increase the urine pH levels in individuals with lower-than-average pH levels.

Key words: Dietary supplements, Urinalysis, Acidosis, Pilot study

Figure 1

pH Measurements during and after supplementation with Reserveage Wholeganic GreensTM"

[1] Visveswaran K . Essentials of nephrology. 2nd ed. New Delhi:BI Publications Pvt Ltd. 2009.
[2] Taal MW, Chertow GM, Marsden PA, Skorecki K, Yu ASL, Brenner BM . Brenner & Rector’s the kidney. 9th ed. Philadelphia:Elsevier Saunders. 2012.
[3] Charles JC, Heilman RL . Metabolic acidosis[J]. Hosp Physician, 2005,41(3):37-42
[4] Welch AA, Bingham SA, Reeve J, Khaw KT . A more acidic dietary acid-base load is associated with reduced calcaneal broadband ultrasound attenuation in women but not men: results from the EPIC-Norfolk cohort study[J]. Am J Clin Nutr, 2007,85(4):1134-1141
doi: 10.1093/ajcn/85.4.1134
[5] New SA , MacDonald HM, Campbell MK, Martin JC, Garton MJ, Robins SP, Reid DM.Lower estimates of net endogenous non-carbonic acid production are positively associated with indexes of bone health in premenopausal and perimenopausal women[J]. Am J Clin Nutr, 2004,79(1):131-138
doi: 10.1093/ajcn/79.1.131
[6] MacDonald HM, New SA, Fraser WD, Campbell MK, Reid DM . Low dietary potassium intakes and high dietary estimates of net endogenous acid production are associated with low bone mineral density in premenopausal women and increased markers of bone resorption in postmenopausal women[J]. Am J Clin Nutr, 2005,81(4):923-933
doi: 10.1093/ajcn/81.4.923
[7] Alexy U, Remer T, Manz F, Neu CM, Schoenau E . Long-term protein intake and dietary potential renal acid load are associated with bone modeling and remodeling at the proximal radius in healthy children[J]. Am J Clin Nutr, 2005,82(5):1107-1114
doi: 10.1093/ajcn/82.5.1107
[8] Buclin T, Cosma M, Appenzeller M, Jacquet AF, Décosterd LA, Biollaz J, Burckhardt P . Diet acids and alkalis influence calcium retention in bone[J]. Osteoporos Int, 2001,12(6):493-499
doi: 10.1007/s001980170095
[9] Schwalfenberg GK . The alkaline diet: is there evidence that an alkaline pH diet benefits health?[J] Environ Public Health. 2012; 2012:727630.
[10] Kanbara A, Hakoda M, Seyama I . Urine alkalization facilitates uric acid excretion[J]. Nutr J, 2010,9:45
doi: 10.1186/1475-2891-9-45
[11] Aveda MM, Souto G . Diet-induced metabolic acidosis[J]. Clin Nutr, 2011,30(4):416-421
doi: 10.1016/j.clnu.2011.03.008
[12] Frassetto LA, Morris RC Jr, Sebastian A . A practical approach to the balance between acid production and renal acid excretion in humans[J]. J Nephrol, 2006,19(Suppl 9):S33-S40
[13] Remer T, Manz F . Potential renal acid load of foods and its influence on urine pH[J]. J Am Diet Assoc, 1995,95(7):791-797
doi: 10.1016/S0002-8223(95)00219-7
[14] Remer T . Influence of nutrition on acid-base balance - metabolic aspects[J]. Eur J Nutr, 2001,40(5):214-220
doi: 10.1007/s394-001-8348-1
[15] Frassetto LA, Todd KM, Morris RC Jr, Sebastian A . Estimation of net endogenous noncarbonic acid production in humans from diet potassium and protein contents[J]. Am J Clin Nutr, 1998,68(3):576-583
doi: 10.1093/ajcn/68.3.576
[16] Vormann J, Remer T . Dietary, metabolic, physiologic, and disease-related aspects of acid-base balance: foreword to the contributions of the second International Acid-Base Symposium.[J] Nutr. 2008; 138(2):413S-414S.
doi: 10.1093/jn/138.2.413S
[17] Pizzorno J, Frassetto LA, Katzinger J . Diet-induced acidosis: is it real and clinically relevant? Br[J] Nutr. 2010; 103(8):1185-1194.
[18] Pagana KD, Pagana TJ . Mosby’s manual of diagnostic and laboratory tests. 4th ed. St. Louis: Elsevier Health Sciences. 2009.
[19] Welch AA, Mulligan A, Bingham SA, Khaw KT . Urine pH is an indicator of dietary acid-base load, fruit and vegetables and meat intakes: results from the European Prospective Investigation into Cancer and Nutrition(EPIC)-Norfolk population study[J]. Br J Nutr, 2008,99(6):1335-1343
[20] Ball D, Maughan RJ . Blood and urine acid-base status of premenopausal omnivorous and vegetarian women[J]. Br J Nutr, 1997,78(5):683-693
doi: 10.1079/BJN19970187
[21] K?nig D, Muser K, Dickhuth HH, Berg A, Deibert P . Effect of a supplement rich in alkaline minerals on acid-base balance in humans[J]. Nutr J, 2009,8:23
doi: 10.1186/1475-2891-8-23
[22] Berardi JM, Logan AC, Rao AV . Plant based dietary supplement increases urinary pH[J]. J Int Soc Sports Nutr, 2008,5:20
doi: 10.1186/1550-2783-5-20
[23] Reddy ST, Wang CY, Sakhaee K, Brinkley L, Pak CYC . Effect of low-carbohydrate high-protein diets on acid-base balance, stone-forming propensity, and calcium metabolism[J]. Am J Kidney Dis, 2002,40(2):265-274
doi: 10.1053/ajkd.2002.34504
[24] Hanna Instruments. HI 98107 pHep? pH Tester.[ 2013-03-06]. .
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