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Journal of Integrative Medicine ›› 2021, Vol. 19 ›› Issue (4): 362-373.doi:

• Original Experimental Research • Previous Articles    

Antioxidant and antiaging effect of traditional Thai rejuvenation medicines in Caenorhabditis elegans 

Sutticha Ruangchuaya,b, Qiang-qiang Wangb, Liang-yi Wangb,c, Jing Linb,c, Yong-chao Wangc, Guo-huan Zhongc, Katesarin Maneenoona, Ze-bo Huangb,c, Sasitorn Chusria,d   

  1. a. Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand 
    b. School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, China
    c. Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, China
    d. Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand

  • Online:2021-08-15 Published:2021-03-24
  • Contact: Sutticha Ruangchuay


This study explored the rejuvenation mechanisms of Thai polyherbal medicines using different approaches, including in vitro methods, as well as a well-defined nematode model, Caenorhabditis elegans.


THP-R-SR012 decoction was selected from 23 polyherbal medicines, based on metal-chelating and chain-breaking antioxidant capacities. The influences of this extract on the survival and some stress biomarkers of C. elegans under paraquat-induced oxidative stress were evaluated. Furthermore, lifespan analysis and levels of lipofuscin accumulation were examined in senescent nematodes. The phytochemical profile of THP-R-SR012 was analyzed. 


Supplementation with THP-R-SR012 decoction significantly increased the mean lifespan and reduced the oxidative damage to C. elegans under oxidative stress conditions. Further, THP-R-SR012 supplementation slightly influenced the lifespan and the level of lipofuscin accumulation during adulthood. Antioxidant-related phytochemical constituents of THP-R-SR012 decoction were rutin, naringenin, 3,4-dihydroxybenzoic acid, gallic acid, glycyrrhizic acid, demethoxycurcumin and 18α-glycyrrhetinic acid.


The antioxidant potential of THP-R-SR012 was due to its scavenging properties, its enhancement of antioxidant-related enzyme activities, and the presence of the antioxidant-related compound. These results support the traditional use of THP-R-SR012 decoction as a tonic for nourishing and strengthening the whole body.

Key words: Antioxidants, Caenorhabditis elegans, Herbal formula, Lifespan extension, Rejuvenation medicine

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