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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (11): 1057-1060.doi: 10.3736/jcim20091107

• Original Experimental Research • Previous Articles     Next Articles

Determination of isoguanosine in Semen Crotonis Tiglii by high-performance liquid chromatography

Yun Ling , Hong-yu Zhao, Xin-liang Pan, Chun-xiang Zhou   

  1. Division of Shanghan and Jingui, Preclinical Medicine College,Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China
  • Received:2009-06-04 Accepted:2009-09-07 Online:2009-11-20 Published:2009-11-15
  • Contact: Chun-xiang Zhou


To develop a high-performance liquid chromatographic method for the determination of isoguanosine in Semen Crotonis Tiglii.

The determination was done by using reverse-phase high-performance liquid chromatography with a Hedera ODS-2 column (4.6 mm ×250 mm, 5 μm). Elution was employed with the mobile phase of acetonitrile-methanol-water (1︰4︰95) at flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm and the column temperature was 25 ℃. Injection volume was 10 μL.

The linear range of isoguanosine was from 0.161 to 0.967 mg. The correlation coefficient of the calibration curves was 0.999 8. The average recovery rate was 98.78% with relative standard deviation of 0.02%. Conclusion: The results show that the method is simple, accurate, and repeatable, and it is suitable for quality control of Semen Crotonis Tiglii.

Key words: High-performance liquid chromatography, Semen Crotonis Tiglii, Isoguanosine

Table 1

Recovery rates of isoguanosine"

Semen Crotonis
Tiglii (g)
isoguanosine (mg)
Added reference
substance amount (mg)
amount (mg)
rate (%)
recovery rate
0.3 1.613 2 1.612 3.198 6 98.35
0.3 1.619 7 1.612 3.176 3 96.56
0.3 1.615 4 1.612 3.258 8 101.95 98.78% 0.02%
0.3 1.617 5 1.612 3.228 4 99.93
0.3 1.615 4 1.612 3.163 7 96.05
0.3 1.613 2 1.612 3.222 7 99.84

Table 2

Determination of isoguanosine in Semen Crotonis Tiglii ($\bar{x}$±s,mg/g)"

No. Habitat n Batch No. Concentration
1 Yunnan 3 20080906 12.0±1.4
2 Yunnan 3 20081145 11.9±1.8
3 Yunnan 3 20081028 16.2±0.5
4 Sichuan 3 080301 13.4±1.4
5 Guangxi 3 080815 10.8±1.8
6 Sichuan 3 080816 11.2±1.5
7 Sichuan 3 080817 11.7±0.4
8 Sichuan 3 081206 12.4±1.8
9 Yunnan 3 090205 10.2±0.6
10 Gansu 3 090210 10.6±1.7

Figure 1

HPLC chromatograms of reference substance and sample A: Reference substance; B: Sample; 1: Isoguanosine."

[1] China Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China(Part one).(2005 ed)[M]. Beijing: Chemical Industry Press, 2005,( 2005 ed):55
国家药典委员会. 中华人民共和国药典(一部)(2005年版)[M]. 北京: 化学工业出版社, 2005,( 2005年版):55
[2] Zhang JX, Wang Y . The contrast experiment on crude and processed Semen Crotonis Tiglii[J]. Zhong Yao Cai, 1992,15(9):29-30
张静修, 王毅 . 生, 熟巴豆对比实验[J]. 中药材, 1992,15(9):29-30
[3] Li JR, Zhu J, Gao XS . Effects of compatibility with Croton tiglium on decanta efficacy of Radix et Rhizoma Rhei[J]. Zhong Cheng Yao, 1993,15(5):26-27
李建荣, 朱江, 高晓山 . 配伍对大黄致泻作用的影响(Ⅱ)——大黄与巴豆配伍[J]. 中成药, 1993,15(5):26-27
[4] Zhao M, Zhao QZ, Zhang CY, Hou C, Guo JL, Wang P, Liu LX, Yao HY, Yu SY . Effects of carcinogen DEN and tumor promotor croton oil on rat liver alpha1-inhibitor 3 gene expression in vivo[J]. Sheng Wu Hua Xue Za Zhi, 1992,8(6):730-734
赵玫, 赵清正, 张春燕, 侯充, 郭金利, 王萍, 刘立新, 姚红芸, 于树玉 . 致癌剂DEN、促癌剂巴豆油对大鼠肝α1抑制因子3基因表达的影响[J]. 生物化学杂志, 1992,8(6):730-734
[5] Sun SS, Zhao YJ, Yuan SQ . Effects of Badoushuang(BDS) on anti-inflammation, immune function, analgesic action and mutation[J]. Zhong Yao Yao Li Yu Lin Chuang, 1993,9(3):36-38
孙颂三, 赵燕洁, 袁士琴 . 巴豆霜对抗炎、免疫、镇痛及致突变的影响[J]. 中药药理与临床, 1993,9(3):36-38
[6] Liu XD, Sui ZY . Effects of Croton tiglium total alkaloids on cancer cell cytoplasm membrane fluidity and structure of the cytoplasmic matrix[J]. Shandong Zhong Yi Xue Yuan Xue Bao, 1995,19(3):192-194
刘秀德, 隋在云 . 巴豆总生物碱对癌细胞质膜流动性及胞浆基质结构的影响[J]. 山东中医学院学报, 1995,19(3):192-194
[7] Tian YW . Differentiation-inducing effect of the Croton tiglium aquatic extracts to HL-60 cells[J]. Shanxi Yi Yao Za Zhi, 2002,31(3):265
doi: 10.3969/j.issn.0253-9926.2002.03.061
田艳伟 . 巴豆水提液对HL-60细胞的诱导分化作用[J]. 山西医药杂志, 2002,31(3):265
doi: 10.3969/j.issn.0253-9926.2002.03.061
[8] Xu JG, Ma JY, Yang GS, Ren LS, Guo YD, Zhang HX . Study on differentiation-inducing effects of Croton tiglium decoction on human promyelocytes[J]. Zhonghua Xue Ye Xue Za Zhi, 1990,11(10):538-539
徐建国, 马俊英, 杨贵生, 任连生, 郭亚东, 张鸿翔 . 巴豆煎液对人早幼粒细胞白血病细胞的诱导分化研究[J]. 中华血液学杂志, 1990,11(10):538-539
[9] Hu J, Gao WY, Ling NS, Shi SX, Wang WY, Zhang TJ . Volatility elements of Croton tiglium and Crotonis pulveratum analyzed by GC-MS[J]. Zhongguo Zhong Yao Za Zhi, 2008,33(4):464-465
doi: 10.3321/j.issn:1001-5302.2008.04.037
胡静, 高文远, 凌宁生, 石世学, 王文燕, 张铁军 . 巴豆和巴豆霜挥发性成分的GC-MS分析[J]. 中国中药杂志, 2008,33(4):464-465
doi: 10.3321/j.issn:1001-5302.2008.04.037
[10] Kim JH, Lee SJ, Han YB, Moon JJ, Kim JB . Isolation of isoguanosine from Croton tiglium and its antitumor activity[J]. Arch Pharm Res, 1994,17(2):115-118
doi: 10.1007/BF02974234 pmid: 10319142
[11] Tang GY, Chen MH, Wu HJ . Isolation and determination of crotin toxin by high performance gel filtration chromatography(GFC) with photodiode array detector[J]. Se Pu, 1994,12(4):244-246
唐根源, 陈明晃, 吴红京 . 高效凝胶过滤色谱法(光电二极管阵列检测器)分离、测定巴豆毒蛋白[J]. 色谱, 1994,12(4):244-246
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