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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 338-342.doi: 10.3736/jcim20070321

• Original Experimental Research • Previous Articles     Next Articles

Expressions of epidermal growth factor receptor signaling substances in gastric mucosal cells influenced by serum derived from rats treated with electroacupuncture at stomach meridian acupoints

Jie Yan, Zong-bao Yang, Xiao-rong Chang, Shou-xiang Yi, Ya-ping Lin, Yan Zhong   

  1. College of Acupuncture and Massage , Hunan University of T raditional Chinese Medicine , Changsha , Hunan Province 410007 ,China
  • Online:2007-05-31 Published:2007-05-15

Objective: To study the effects of serum derived from rats treated with electroacupuncture at stomach meridian acupoints on the expressions of epidermal growth factor receptor (EGFR) signaling substances phospholipase Cγ-1 (PLCγ-1), protein kinase C (PKC) and c-myc in gastric mucosal cells.Methods: Sixty rats were randomly divided into normal group, stomach meridian group, gallbladder meridian group, stomach meridian plus PD153035 group and gallbladder meridian plus PD153035 group. Water-immersion and restrained stress methods were adopted for inducing gastric mucosal injury in the rats. Gastric mucosal cells were separated by using pronase digestion method, and incubated by PD153035, a EGFR inhibitor, and 100 ml/L serum. The expression of PLCγ-1 in the gastric mucosal cells was tested by enzyme linked-immunosorbent assay (ELISA), while the expression of PKC by isotope incorporate assay and the expression of c-myc by reverse transcription polymerase chain reaction assay (RT-PCR).Results: In gastric mucosal cells, weak expressions of PLCγ-1, PKC and c-myc were seen in the normal group, and relatively strong expressions of PLCγ-1, PKC and c-myc were seen in the stomach meridian group and the gallbladder meridian group, among which, the expressions of PLCγ-1, PKC and c-myc in the stomach meridian group were the strongest, and there was a significant difference between the stomach meridian group and the gallbladder meridian group (P<0.01). Relative weak expressions of PLCγ-1, PKC and c-myc were seen in the stomach meridian plus PD153035 group and the gallbladder meridian plus PD153035 group, and there was a significant difference between the stomach meridian group and the stomach meridian plus PD153035 group (P<0.01).Conclusions: The serum derived from the rats treated with electroacupuncture at stomach meridian acupoints can activate the EGFR singling pathway, and this provides an evidence for the theory of "relative particularity between meridians and viscera" in traditional Chinese medicine.

Table 1

Expressions of PKC, c-myc gene and PLCγ-1 in gastric mucosal cells in 5 groups(ヌ±S)"

GroupnPLCγ-1[nmol/(g·min)]PKC[nmol/(g·min)]c-myc gene
Normal8390.45±120.8417.49±18.560.804 2±0.070 5
Stomach meridian8778.90±449.78**68.94±39.36**1.089 4±0.022 7**
Gallbladder meridian8503.84±232.40**△△21.88±16.52△△1.004 2±0.033 3**△△
Stomach meridian + PD1530358332.32±119.20**△△▲▲10.43±14.03△△0.920 0±0.034 3**△△▲
Gallbladder meridian + PD1530358430.46±107.98**△△▲▲14.06±8.65△△0.911 4±0.038 5**△△▲▲

Figure 1

Electrophoretogram of c-myc gene and GAPDH gene RT-PCR product in gastric mucosal cellsM: Marker; A: Normal group; B: Stomach meridian group; C: Gallbladder meridian group; D: Stomach meridian + PD153035 group; E: Gallbladder meridian + PD153035 group."

[1] 高群, 曹曙波, 蔺虹, 等.针灸治疗肝气郁结型消化性溃疡131例疗效观察[J].针灸临床杂志,2004,20(4):10-12
Gao Q, Cao SB, Lin H, et al.Effect of acupuncture therapy in 131 patients with peptic ulcer of stagnation of liver-qi type[J].Zhen Jiu Lin Chuang Za Zhi,2004,20(4):10-12
[2] Yan J, Yang RD, He JF, et al.Effect of acupuncture at different meridian acupoints on changes of related factors for rabbit gastric mucosal injury[J].World J Gastroenterol,2005,11(41):6472-6476
[3] 刘建平, 卜涛, 白建乐, 等.EGF、TGF-α及EGFR在大鼠胃溃疡自愈过程中的表达及意义[J].中国药理学通报,2003,19(10):1185-1188
Liu JP, Bu T, Bai JL, et al.The expression and significance of EGF, TGF-α and EGFR in the nature concrescene course of rats gastric ulcer[J].Zhongguo Yao Li Xue Tong Bao,2003,19(10):1185-1188
[4] 南开大学实验动物解剖学编写组.实验动物解剖学(第2版)[M].北京: 人民教育出版社,1983,(第2版):174-178
Editing Group for Experimental Animal Anatomy of Nankai University.Experimental animal anatomy(2nd ed)[M].Beijing: People's Education Press,1983,(2nd ed):174-178
[5] Konturek PC, Brzozowski T, Konturek SJ, et al.Activation of genes for growth factors and cyclooxygenases in rat gastric mucosa during recovery from stress damage[J].Eur J Pharmacol,1998,342(1):55-65
[6] Everett SM, White KL, Schorah CJ, et al.In vivo DNA damage in gastric epithelial cells[J].Mutat Res,2000,486(1):73-85
doi: 10.1016/S1383-5718(00)00041-3 pmid: 10863159
[7] 牟向东, 谢鹏雁, 刘建湘, 等.电针刺大鼠足三里穴对LESP血浆胃泌素和胃动素的影响[J].世界华人消化杂志,2005,13(9):1069-1073
Mu XD, Xie PY, Liu JX, et al.Effect of electro-acupuncture at Zusanli acupoint on LESP, plasma gastrin and motilin in rats[J].Shi Jie Hua Ren Xiao Hua Za Zhi,2005,13(9):1069-1073
[8] 常小荣, 严洁, 易受乡, 等.电针足三里对大鼠胃电及脑肠肽的影响[J].中医药学刊,2004,22(2):220, 240
Chang XR, Yan J, Yi SX, et al.Effects of electro-acupuncture at Zusanli acupoint on gastric electrical activities and brain-gut peptides in rats[J].Zhong Yi Yao Xue Kan,2004,22(2):220, 240
[9] 林亚平, 易受乡, 严洁, 等.电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响[J].中国中西医结合消化杂志,2003,11(2):75-77
Ling YP, Yi SX, Yan J, et al.Effect of stimulating points of the stomach meridian of foot-yangming by electronic needle on gastric mucosal blood flow and somatostatin in the rat model of gastric mucosal injury[J].Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi,2003,11(2):75-77
[10] 张炜宁, 张烨, 李家邦.健胃愈疡颗粒剂对胃溃疡患者胃黏膜表皮生长因子受体表达的影响[J].中西医结合学报,2004,2(1):24-26
Zhang WN, Zhang Y, Li JB.Effect of Jianwei Yuyang Granules on expression of epidermal growth factor receptor in gastric mucosa of gastric ulcer patients[J].Zhong Xi Yi Jie He Xue Bao,2004,2(1):24-26
[11] Xiao ZQ, Li J, Majumdar AP.Regulation of TGF-alpha-induced activation of AP-1 in the aging gastric mucosa[J].Am J Physiol Gastrointest Liver Physio,2003,285(2):G396-G403
doi: 10.1152/ajpgi.00530.2002 pmid: 12672650
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