Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 416-420.doi: 10.3736/jcim20070411

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbs for replenishing qi and resolving stagnation on transforming growth factor-β1 of skin ulcers in rats with diabetes

Zhen Zhang, Hua-fa Que, Yuan-ying Zhu, Yun-fei Wang, Xiao-he Liu, Pei-yong Zheng   

  1. Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Online:2007-07-31 Published:2007-05-15

Objective: To explore the effects of Yiqi Huayu Recipe, a compound traditional Chinese herbal medicine for replenishing qi and resolving stagnation, on transforming growth factor-β1(TGF-β1) of skin ulcers in rats with diabetes.Methods: Sixty rats were randomly divided into six groups, Yiqi Huayu Recipe-treated group, Yiqi (replenishing qi) Recipe-treated group, Huayu (resolving stagnation) Recipe-treated group, basic fibroblast growth factor (bFGF) treated group, untreated group and normal control group. Diabetes was induced by peritoneal injection of streptozotocin and skin ulcers were made by surgery method in rats except for the normal control group. Then the rats were administered with different drugs respectively, and the expression of TGF-β1 in granulation tissue of the skin ulcers was detected with the methods of Western blotting, image analysis and immunohistochemistry.Result: The level of TGF-β1 expression in the untreated group was lower than that in the normal control group (P<0.01); and the level of TGF-β1 expression in the drug-treated groups was higher than that in the untreated group (P<0.01); and the TGF-β1 expression in the Yiqi Huayu Recipe-treated group was higher than that in the Yiqi Recipe-treated group, Huayu Recipe-treated group and bFGF-treated group (P<0.05).Conclusion: The replenishing qi and resolving stagnation therapy can control the secretion of TGF-β1 of the wound in the process of wound healing in the levels of gene and molecule.

Key words: diabetes mellitus, skin ulcers, transforming growth factor beta1, rats

CLC Number: 

  • R285

Table 1

TGF-β1 expression of skin ulcers in rats with diabetes"

GroupImmunohistochemical method (μm2)(n=8)Western blotting (n=6)
Yiqi Huayu Recipe-treated17 219.37±2 658.74**▲□■220.17±13.76**▲▲□□■■
Yiqi Recipe-treated13 977.01±3 072.60**150.00±5.06**
Huayu Recipe-treated13 954.54±2 552.99**151.00±5.93**
bFGF-treated14 006.89±1 798.88182.00±7.32**
Untreated9 859.68±1 940.99△△78.48±8.85
Normal control15 385.41±3 248.42**221.50±12.72**▲▲□□■■

Figure 1

Expression of TGF-β1 in granulation tissue of the rats in six groups seven days after skin lesion (SP, ×100)A: Yiqi Huayu Recipe-treated; B: Yiqi Recipe-treated; C: Huayu Recipe-treated; D: Untreated; E: bFGF-treated; F: Normal control."

Figure 2

Expression of TGF-β1 in granulation tissue of the rats in six samples seven days after skin lesion (Western blotting)A: Yiqi Huayu Recipe-treated; B: Yiqi Recipe-treated; C: Huayu Recipe-treated; D: Untreated; E: bFGF-treated; F: Normal control."

[1] 阙华发, 唐汉钧, 向寰宇, 等.益气化瘀法治疗糖尿病皮肤溃疡38例临床观察[J].中西医结合学报,2004,2(1):63-64
Que HF, Tang HJ, Xiang HY, et al.Treatment of diabetic skin ulcers by invigorating qi and dispersing stagnation: A report of 38 cases[J].Zhong Xi Yi Jie He Xue Bao,2004,2(1):63-64
[2] 陈奇. 中药药理实验方法学[M].北京: 人民卫生出版社,1993,():176
Chen Q.Experimental methods of Chinese traditional medicinal pharmacology[M].Beijing: People's Medical Publishing House,1993,():176
[3] 付小兵, 孙同柱, 盛志勇.几种用于创伤修复研究的动物模型[J].中华实验外科杂志,1999,16(5):479-480
Fu XB, Sun TZ, Sheng ZY.Some kinds of animal models for study of trauma repairing[J].Zhonghua Shi Yan Wai Ke Za Zhi,1999,16(5):479-480
[4] 阙华发, 唐汉钧, 王林扬, 等.益气化瘀法促进慢性难愈性创面修复愈合的机制研究[J].中西医结合学报,2005,3(3):243-246
Que HF, Tang HJ, Wang LY, et al.Study on wound healing mechanism of replenishing qi and dissipating stagnation therapy for chronic skin ulcers[J].Zhong Xi Yi Jie He Xue Bao,2005,3(3):243-246
[5] 屈纪富, 郝利, 孙薇, 等.细胞因子在创伤愈合过程中的变化及其意义的研究进展[J].创伤外科杂志,2003,5(1):74-76
Qu JF, Hao L, Sun W, et al.Retrospective study on changes of cytokines in processes of wound healing and its significance[J].Chuang Shang Wai Ke Za Zhi,2003,5(1):74-76
[6] 黄君富, 房殿春, 鲁荣.TGF-β家族的细胞信号转导[J].生理科学进展,1999,30(3):255-258
Huang JF, Fang DC, Lu R.Cell signal transduction of TGF-β[J].Sheng Li Ke Xue Jin Zhan,1999,30(3):255-258
[7] 付小兵, 程飚.伤口愈合的新概念[J].中国实用外科杂志,2005,25(1):29-32
Fu XB, Chen B.New conception of wound healing[J].Zhongguo Shi Yong Wai Ke Za Zhi,2005,25(1):29-32
[8] Cromack DJ, Sporn MB, Roberts AB, et al.Transforming growth factor beta levels in rat wound chambers[J].J Surg Res,1987,42(6):622-628
doi: 10.1016/0022-4804(87)90005-9
[9] 宋雅芳, 洪嘉禾, 徐列明.中医药对TGF-β1调控作用的研究进展[J].上海中医药杂志,2004,38(1):61-64
Song YF, Hong JH, Xu LM.Progress of the regulation of TGF-β1 by Chinese medicine[J].Shang Hai Zhong Yi Yao Za Zhi,2004,38(1):61-64
[10] 刘志君, 徐辉.转化生长因子-β与创伤愈合[J].重庆医学,2004,33(6):927-929
Liu ZJ, Xu H.Transforming growth factor-β and wound healing[J].Chongqing Yi Xue,2004,33(6):927-929
[1] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[2] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[3] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[4] Olakunle Bamikole Afolabi, Omotade Ibidun Oloyede, Shadrack Oludare Agunbiade. Inhibitory potentials of phenolic-rich extracts from Bridelia ferruginea on two key carbohydrate-metabolizing enzymes and Fe2+-induced pancreatic oxidative stress. Journal of Integrative Medicine, 2018, 16(3): 192-198.
[5] Chun-song Hu, Tengiz Tkebuchava. SEEDi1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[6] Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[7] Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[8] Yee Chi Peggy Wong. Need of integrated dietary therapy for persons with diabetes mellitus and “unhealthy” body constitution presentations. Journal of Integrative Medicine, 2016, 14(4): 255-268.
[9] Ausanee Wanchai, Duangjai Phrompayak. Use of complementary and alternative medicine among Thai patients with type 2 diabetes mellitus. Journal of Integrative Medicine, 2016, 14(4): 297-305.
[10] Sulaiman Al-Eidi, SalwaTayel, Fatima Al-Slail, Naseem Akhtar Qureshi, Imen Sohaibani, Mohamed Khalil, Abdullah Mohammad Al-Bedah. Knowledge, attitude and practice of patients with type 2 diabetes mellitus towards complementary and alternative medicine. Journal of Integrative Medicine, 2016, 14(3): 187-196.
[11] Mohammad Hashem Hashempur, Mojtaba Heydari, Seyed Hamdollah Mosavat, Seyyed Taghi Heydari, Mesbah Shams. Complementary and alternative medicine use in Iranian patients with diabetes mellitus. Journal of Integrative Medicine, 2015, 13(5): 319-325.
[12] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[13] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[14] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[15] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[4] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[5] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[6] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[7] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[8] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[9] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[10] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76