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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 416-420.doi: 10.3736/jcim20070411

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbs for replenishing qi and resolving stagnation on transforming growth factor-β1 of skin ulcers in rats with diabetes

Zhen Zhang, Hua-fa Que, Yuan-ying Zhu, Yun-fei Wang, Xiao-he Liu, Pei-yong Zheng   

  1. Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Online:2007-07-31 Published:2007-05-15

Objective: To explore the effects of Yiqi Huayu Recipe, a compound traditional Chinese herbal medicine for replenishing qi and resolving stagnation, on transforming growth factor-β1(TGF-β1) of skin ulcers in rats with diabetes.Methods: Sixty rats were randomly divided into six groups, Yiqi Huayu Recipe-treated group, Yiqi (replenishing qi) Recipe-treated group, Huayu (resolving stagnation) Recipe-treated group, basic fibroblast growth factor (bFGF) treated group, untreated group and normal control group. Diabetes was induced by peritoneal injection of streptozotocin and skin ulcers were made by surgery method in rats except for the normal control group. Then the rats were administered with different drugs respectively, and the expression of TGF-β1 in granulation tissue of the skin ulcers was detected with the methods of Western blotting, image analysis and immunohistochemistry.Result: The level of TGF-β1 expression in the untreated group was lower than that in the normal control group (P<0.01); and the level of TGF-β1 expression in the drug-treated groups was higher than that in the untreated group (P<0.01); and the TGF-β1 expression in the Yiqi Huayu Recipe-treated group was higher than that in the Yiqi Recipe-treated group, Huayu Recipe-treated group and bFGF-treated group (P<0.05).Conclusion: The replenishing qi and resolving stagnation therapy can control the secretion of TGF-β1 of the wound in the process of wound healing in the levels of gene and molecule.

Key words: diabetes mellitus, skin ulcers, transforming growth factor beta1, rats

CLC Number: 

  • R285

Table 1

TGF-β1 expression of skin ulcers in rats with diabetes"

GroupImmunohistochemical method (μm2)(n=8)Western blotting (n=6)
Yiqi Huayu Recipe-treated17 219.37±2 658.74**▲□■220.17±13.76**▲▲□□■■
Yiqi Recipe-treated13 977.01±3 072.60**150.00±5.06**
Huayu Recipe-treated13 954.54±2 552.99**151.00±5.93**
bFGF-treated14 006.89±1 798.88182.00±7.32**
Untreated9 859.68±1 940.99△△78.48±8.85
Normal control15 385.41±3 248.42**221.50±12.72**▲▲□□■■

Figure 1

Expression of TGF-β1 in granulation tissue of the rats in six groups seven days after skin lesion (SP, ×100)A: Yiqi Huayu Recipe-treated; B: Yiqi Recipe-treated; C: Huayu Recipe-treated; D: Untreated; E: bFGF-treated; F: Normal control."

Figure 2

Expression of TGF-β1 in granulation tissue of the rats in six samples seven days after skin lesion (Western blotting)A: Yiqi Huayu Recipe-treated; B: Yiqi Recipe-treated; C: Huayu Recipe-treated; D: Untreated; E: bFGF-treated; F: Normal control."

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