Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (4): 427-431.doi: 10.3736/jcim20070413

• Original Experimental Research • Previous Articles     Next Articles

Effects of Shenfu Injection on prostacyclin, thromboxane A2 and activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury

Song-lin Peng1, Xi Gu1, Chao-liu Dai1, Yong Huang2, Yong Zhao1   

  1. 1. Department of Hepatobiliary and Mammary Surgery, The Second Hospital, China Medical University, Shenyang, Liaoning Province 110004, China  2. Department of General Surgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong Province 271100, China
  • Online:2007-07-31 Published:2007-05-15

Objective: To explore the effects of Shenfu Injection on prostacyclin, thromboxane A2 and the activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury.Methods: Twenty-four male Wistar rats weighing 200-250 g were randomly divided into two groups: Shenfu Injection (SF)-treated group (rats were treated with Shenfu Injection of 10 ml/kg through intraperitoneal injection) and untreated group (rats were administered with normal saline at the same dose and served as a control group). Hepatic ischemia was caused by Pringle's maneuver and lasted for fifteen minutes, and then one-hour or three-hour reperfusion was performed. Venous blood samples for the measurement of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) were collected three hours after reperfusion. Liver tissue samples were collected one hour or three hours after reperfusion for the measurement of Na+-K+-ATPase and Ca2+-Mg2+-ATPase and for morphological studies.Result: Plasma TXB2 was lower in the SF-treated group than that in the untreated group after three-hour reperfusion (P>0.05), while 6-keto-PGF1α was higher in the SF-treated group than that in the untreated group (P>0.05). The ratio of TXB2 and 6-keto-PGF1α was significantly lower in the SF-treated group than that in the untreated group (P<0.05). The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in the SF-treated group were improved obviously. A three-hour reperfusion after fifteen-minute ischemia caused important hepatic histological alterations. Marked structural abnormalities were observed in the untreated group, such as massive hepatocyte swelling, necrosis, mitochondria edema and vacuolar changes. In the SF-treated group, hepatic tissue injury was reduced significantly.Conclusion: Shenfu Injection protects hepatic tissue from ischemia-reperfusion injury, and such protective effects are achieved by decreasing the ratio of thromboxane A2 and prostacyclin, and increasing the activities of Na+-K+-ATPase and Ca2+-Mg2+- ATPase.

Key words: ischemia-reperfusion injury, thromboxane A2, prostacyclin, Na+-K+-ATPase, Ca2+-Mg2+-ATPase

CLC Number: 

  • R657.3

Table 1

Plasma concentrations of TXB2 and 6-keto-PGF1α and their ratio after three-hour reperfusion(ヌ±S)"

Groupn TXB2(ng/L)6-keto-PGF1a(ng/L)TXB2/6-keto-PGF1a
SF-treated6118.7±19.11 081.7±282.70.11±0.03*

Table 2

Levels of hepatic tissue ATPases after one- or three-hour reperfusion[ヌ±S, μmol Pi/(mg prot·h)]"

Groupn1 h3 h

Figure 1

Massive hepatocyte swelling, necrosis, sinusoidal and microvascular congestion in untreated group (HE staining, ×100)"

Figure 2

Sinusoidal congestion and tissue injury were reduced significantly in SF-treated group (HE staining, ×100)"

Figure 3

Severe injuries of hepatocytes and mitochondria in untreated group (transmission electron microscope, ×8 000)"

Figure 4

Injuries of hepatocytes and mitochondria were reduced in SF-treated group (transmission electron microscope, ×8 000)"

[1] Kondo T, Todoroki T, Hirano T, et al.Impact of ischemia-reperfusion injury on dimensional changes of hepatic microvessels[J].Res Exp Med (Berl),1998,198(2):63-72
doi: 10.1007/s004330050090 pmid: 9782526
[2] Xiao CY, Hara A, Yuhki K, et al.Roles of prostaglandin I2 and thromboxane A2 in cardiac ischemia-reperfusion injury: a study using mice lacking their respective receptors[J].Circulation,2001,104(18):2210-2215
[3] Schubert R, Serebryakov VN, Mewes H, et al.Iloprost dilates rat small arteries: role of K(ATP)- and K(Ca)-channel activation by cAMP-dependent protein kinase[J].Am J Physiol,1997,272(3 pt 2):H1147-H1156
[4] Node K, Kitakaze M, Kosaka H, et al.Bradykinin mediation of Ca 2+-activated K+ channels regulates coronary blood flow in ischemic myocardium[J].Circulation,1997,95(6):1560-1567
[5] Gross GJ, Auchampach JA.Blockade of ATP-sensitive potassium channels prevents myocardial preconditioning in dogs[J].Circ Res,1992,70(2):223-233
doi: 10.1007/BF00789438 pmid: 1310443
[6] de Wit C, Bolz SS, Pohl U.Interaction of endothelial autacoids in microvascular control[J].Z Kardiol,2000,89(suppl 9):Ⅸ/113-116
[7] Myers SI, Horton JW, Hernandez R, et al.Pentoxifylline protects splanchnic prostacyclin synjournal during mesenteric ischemia/reperfusion[J].Prostaglandins,1994,47(2):137-150
[8] Jiang XW, Kambara K, Gotoh N, et al.Effects of low-dose Beraprost sodium, a stable prostaglandin I2 analogue, on reperfusion injury to rabbit lungs[J].Am J Respir Crit Care Med,1998,158(5 Pt 1):1669-1675
[9] 张汝鹏, 王殿昌, 邢福胜.大鼠肝缺血再灌注损伤时血栓素A2和前列环素变化及川芎嗪的作用[J].中国中西医结合外科杂志,1997,3(6):393-395
Zhang RP, Wang DC, Xing FS.Changes of thromboxane and prostaeyclin in liver after ischemia/reperfusion injury and the therapeutic effect of tetramethypyraxine[J].Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi,1997,3(6):393-395
[10] Shirabe K, Kin S, Shinagawa Y, et al.Inhibition of thromboxane A2 activity during warm ischemia of the liver[J].J Surg Res,1996,61(1):103-107
doi: 10.1006/jsre.1996.0088 pmid: 8769950
[11] 包维民, 郭永章, 唐映梅, 等.大鼠肝脏冷/热缺血再灌注损伤钠钾ATP酶、钙ATP酶及镁ATP酶与肝细胞死亡方式的研究[J].中国现代普通外科进展,2004,7(6):343-346
Bao WM, Guo YZ, Tang YM, et al.The effect of cold/warm ischemia reperfusion injury on cell death pattern and the activities of Na+-K+ATPase, Mg2+ATPase and Ca2+ATPase in liver of rats[J].Zhongguo Xian Dai Pu Tong Wai Ke Jin Zhan,2004,7(6):343-346
[12] 谷天祥, 张显清, 谷春久, 等.心肌缺血再灌注损伤亚细胞Ca2+反常与ATP酶泵功能抑制[J].中华心血管病杂志,2001,29(7):420-423
Gu TX, Zhang XQ, Gu CJ, et al.The relationship between subcellular calcium paradox and ATPase pump inhibition in myocardial ischemic and reperfusion injury[J].Zhonghua Xin Xue Guan Bing Za Zhi,2001,29(7):420-423
[13] 郭建荣, 崔健君, 丁节清, 等.异丙酚对缺血再灌注损伤大鼠海马线粒体能量代谢、ATP酶活性及自由基系统的影响[J].中国临床药理学与治疗学,2004,9(8):893-896
Guo JR, Cui JJ, Ding JQ, et al.Effects of propofol on ATP content, ATPase activity and lipid peroxidation of hippocampus mitochondria following global ischemia-reperfusion in rats[J].Zhongguo Lin Chuang Yao Li Xue Yu Zhi Liao Xue,2004,9(8):893-896
[1] Chan Chen, Chang-xun Chen, Xi-min Wu, Rui Wang, Yi-ming Li. Effects of extracts of Radix Scrophulariae on blood pressure in spontaneously hypertensive rats and the underlying mechanisms. Journal of Chinese Integrative Medicine, 2012, 10(9): 1009-1017.
[2] Bin Cheng, Xin Wang, Lei Wang, Kai Cao, Lei Duan, Feng-tao Li. Changes of mitochondrial apoptosis in spinal cord ischemia-reperfusion injury and the effects of Herba Erigerontis Breviscapi Injection preconditioning intervention in rabbits. Journal of Chinese Integrative Medicine, 2009, 7(9): 842-847.
Full text



[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239