Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester-induced hypertensive male rats

doi:10.1016/j.joim.2017.11.001

Abstract

Objective
This study investigated the effects of aqueous leaf extract of Tridax procumbens (ALETP) on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive male rats.

Methods

Twenty normal, adult male rats (130–150?g) were divided into four groups of five rats each. Group I (control) was given normal saline (0.6?mL/kg) and group II was given L-NAME (40?mg/kg) for 6?weeks. Groups III and IV also received L-NAME (40?mg/kg) for 6?weeks but were further co-treated with 100 and 200?mg/kg of ALETP, respectively, from week 4 to week 6. All treatments were given orally. Strips of corpus cavernosum from each of the four groups were exposed to increasing concentrations of acetylcholine (ACh) and sodium nitroprusside (SNP) (10^?9–10^?5 mol/L) after contraction with phenylephrine (10^?7?mol/L) to test for a dose–response effect. Response to potassium and calcium was also measured after cumulatively adding potassium and calcium (10–50?mmol/L) to potassium- and calcium-free organ chamber. Isometric contractions were recorded through an Ugo Basile data capsule acquisition system.

Results

Mean arterial blood pressure was significantly reduced in the ALETP co-treated group compared to the control and L-NAME-only groups (P?<?0.05). Cavernosa strips from ALETP co-treated rats exhibited significant inhibition of contraction in response to phenylephrine, potassium chloride, and calcium chloride (P?<?0.05). Relaxation in response to Ach and SNP was also significantly impaired in cavernosa strips from the L-NAME-only treated group (P?<?0.05), while ALETP co-treated groups showed enhanced percentage relaxation.

Conclusion

ALETP treatment of L-NAME-induced hypertensive rats promotes a relaxant effect on isolated cavernosa strips. ALETP shows potential in correcting erectile dysfunction in hypertension.

Key words: L-arginine methyl ester')">N-Nitro-L-arginine methyl ester, Hypertensive, Tridax procumbens, Corpus cavernosum, Relaxation, Sodium nitroprusside, Acetylcholine

Shakiru Ademola Salami, Hussein Mofomosara Salahdeen, Evangelshane Chukwudubem Ugbebor, Babatunde Adekunle Murtala, Yinusa Raji . Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N-nitro-L-arginine methyl ester-induced hypertensive male rats. Journal of Integrative Medicine, 2018, 16(1): 51-56.

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URL: http://www.jcimjournal.com/EN/10.1016/j.joim.2017.11.001

http://www.jcimjournal.com/EN/Y2018/V16/I1/51

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