Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 880-885.doi: 10.3736/jcim20120808

Previous Articles     Next Articles

Vasodilator effect of oroxylin A on thoracic aorta isolated from rats

Hong Wang1,2(), Jing-tian Qu1, Xin Zhao2, Ying Guo2, Hao-ping Mao1   

  1. 1. Tianjin State Key Laboratory of Modern Chinese Medicine, Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    2. Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese medicine, Tianjin 300193, China
  • Received:2012-06-06 Accepted:2012-06-14 Online:2012-08-20 Published:2018-08-15
  • Contact: Wang Hong E-mail:wanghongsys@126.com

Objective: To investigate the vasodilator effect and the endothelium-dependent mechanism of oroxylin A in thoracic aorta isolated from rats.

Methods: Thoracic aorta was isolated from Wistar rats. After pretreatment with norepinephrine or KCl, the effects of oroxylin A at different concentrations were detected on isolated vascular rings prepared from rats’ thoracic aorta. The response of thoracic aortic ring was evaluated in the presence and absence of endothelium, and N G-nitro-L-arginine methyl ester (L-NAME), a specific inhibitor of nitric oxide synthase.

Results: Oroxylin A (10 and 100 μmol/L) caused vasodilation on endothelium-intact aortic rings pretreated with norepinephrine (1 μmol/L) and KCl (60 mmol/L) compared with the control (P<0.05, P<0.01). The vasodilation function of 10 and 100 μmol/L oroxylin A on the endothelium-denuded aorta rings was significantly lower than that on the endothelium-intact aorta rings (P<0.05, P<0.01). L-NAME pretreatment significantly attenuated the effect of 100 μmol/L oroxylin A on endothelium-intact aorta rings (P<0.05, P<0.01).

Conclusion: Oroxylin A can induce the relaxation of the aorta ring in endothelium-dependent manner. Nitric oxide may be involved in the endothelium-dependent effect of oroxylin A.

Key words: plant extracts, aorta, thoracic, oroxylin A, endothelium, vascular, vasodilation, rats, in vitro

Figure 1

Structure of oroxylin A"

Figure 2

Effects of oroxylin A on contractile function of endothelium-intact aortic rings pretreated with NEData are represented as mean±standard deviation, n=6. *P<0.05, **P<0.01, vs control group. NE: norepinephrine."

Figure 3

Effects of oroxylin A on contractile function of endothelium-intact aortic rings pretreated with KClData are represented as mean±standard deviation, n=6. *P<0.05, **P<0.01, vs control group."

Figure 4

Effects of oroxylin A on contractile function of endothelium-denuded aortic rings pretreated with NEData are represented as mean±standard deviation, n=6. △P<0.05, △△P<0.01, vs +E+oroxylin A group. +E: endothelium intact rings; –E: endothelium-denuded rings; NE: norepinephrine."

Figure 5

Effects of oroxylin A on contractile function of endothelium-denuded aortic rings pretreated with KClData are represented as mean±standard deviation, n=6. △P<0.05, △△P<0.01, vs +E+oroxylin A group. +E: endothelium intact rings; –E: endothelium-denuded rings. "

Figure 6

Effects of L-NAME on the vasodilator function of oroxylin A in aorta rings pretreated with NE Data are represented as mean±standard deviation, n=6. ▲P<0.05, ▲▲P<0.01, vs oroxylin A group. L-NAME: NG-nitro-L-arginine methyl ester; NE: norepinephrine."

Figure 7

Effects of L-NAME on the vasodilator function of oroxylin A in aorta rings pretreated with KClData are represented as mean±standard deviation, n=6. ▲▲P<0.01, vs oroxylin A group. L-NAME: NG-nitro-L-arginine methyl ester."

[1] Li YQ, Feng YL, Yang SL, Xu LZ . Research on chemical constituents of Capparis spinosa L[J]. Zhong Cao Yao, 2007,38(4):510-512
李云秋, 冯育林, 杨世林, 徐丽珍 . 刺山柑化学成分的研究[J]. 中草药, 2007,38(4):510-512
[2] Hang W. Studies on the antitumor active constituents isolated from the root of Ardisia crispa. Changsha: Central South University. 2007. Chinese with abstract in English.
doi: 10.7666/d.y1326340
黄伟 . 百两金抗肿瘤活性成分研究. 长沙: 中南大学. 2007.
doi: 10.7666/d.y1326340
[3] 张中朋, 杨中林, 唐登峰, 孙明珍 . 地蚕化学成分的分离与鉴定[J]. 中成药, 2004,26(12):1051-1053
doi: 10.3969/j.issn.1001-1528.2004.12.022
Zhang ZP, Yang ZL, Tang DF, Sun MZ . Isolation and structure identification of chemical constituents from Stachys geobombycis[J]. Zhong Cheng Yao, 2004,26(12):1051-1053
doi: 10.3969/j.issn.1001-1528.2004.12.022
[4] Yin ZY. Studies on the phytoestrogenic constituents of Eucommia ulmoides Oliv. Tianjin: Tianjin University. 2010. Chinese with abstract in English.
doi: 10.7666/d.y1874368
尹昭晔 . 杜仲的植物雌激素活性成分研究. 天津: 天津大学. 2010.
doi: 10.7666/d.y1874368
[5] Zhao L, Chen Z, Wang J, Yang L, Zhao Q, Wang J, Qi Q, Mu R, You QD, Guo QL . Synergistic effect of 5-fluorouracil and the flavanoid oroxylin A on HepG2 human hepatocellular carcinoma and on H22 transplanted mice[J]. Cancer Chemother Pharmacol, 2010,65(3):481-489
doi: 10.1007/s00280-009-1053-2
[6] Jeon SJ, Rhee SY, Seo JE, Bak HR, Lee SH, Ryu JH, Cheong JH, Shin CY, Kim GH, Lee YS, Ko KH . Oroxylin A increases BDNF production by activation of MAPK-CREB pathway in rat primary cortical neuronal culture[J]. Neurosci Res, 2011,69(3):214-222
doi: 10.1016/j.neures.2010.11.008
[7] Chen Y, Yang L, Lee TJ . Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via suppression of nuclear factor-κB activation[J]. Biochem Pharmacol, 2000,59(11):1445-1457
doi: 10.1016/S0006-2952(00)00255-0
[8] Shih HC, Hsu CS , Yang LL .In vitro study of the tocolytic effect of oroxylin A from Scutellaria baicalensis root[J].J Biomed Sci, 2009, 16: 27
doi: 10.1186/1423-0127-16-27 pmid: 2656494
[9] Trinh HT, Joh EH, Kwak HY, Baek NI, Kim DH . Anti-pruritic effect of baicalin and its metabolites, baicalein and oroxylin A, in mice[J]. Acta Pharmacol Sin, 2010,31(6):718-724
doi: 10.1038/aps.2010.42 pmid: 4002967
[10] Wang H. Study on material basis of Qing’e Formula based on phytoestrogen-like effect. Tianjin: Tianjin University of Traditional Chinese Medicine. 2010. Chinese with abstract in English.
王虹 . 基于植物雌激素样作用的青娥方药效物质基础及作用机理研究. 天津: 天津中医药大学. 2010.
[11] Tep-areenan P, Kendall DA, Randall MD . Mechanisms of vasorelaxation to 17β-oestradiol in rat arteries[J]. Eur J Pharmacol, 2003,476(1-2):139-149
doi: 10.1016/S0014-2999(03)02152-6 pmid: 12969759
[12] Tep-Areenan P, Sawasdee P, Randall M . Possible mechanisms of vasorelaxation for 5,7-dimethoxyflavone from Kaempferia parviflora in the rat aorta[J]. Phytother Res, 2010,24(10):1520-1525
doi: 10.1002/ptr.v24:10
[13] Huang ZX, Yue JL, Zhao FS, Sha DN, Fan XB, Lan XD . Experimental studies on effects of lowering blood pressure and acute toxicity of Viscum coloratum and Eucommia ulmoides[J]. Tian Ran Chan Wu Yan Jiu Yu Kai Fa, 2003,15(3):245-248
doi: 10.3969/j.issn.1001-6880.2003.03.020
黄志新, 岳京丽, 赵凤生, 沙大年, 范小兵, 蓝先德 . 槲寄生、杜仲的降血压作用和急性毒性的实验研究[J]. 天然产物研究与开发, 2003,15(3):245-248
doi: 10.3969/j.issn.1001-6880.2003.03.020
[14] Song Y, Xu JY . Anti-hypertension effect and primary mechanism discussion of Eucommia ulmoides Oliv. Lignans[J]. Zhong Yi Yao Xue Kan, 2006,24(10):1934-1936
宋妍, 许激扬 . 杜仲木脂素化合物降压药效学研究与机制初探[J]. 中医药学刊, 2006,24(10):1934-1936
[15] Riddoch FC, Rowbotham SE, Brown AM, Redfern CP, Cheek TR . Release and sequestration of Ca2+ by a caffeine- and ryanodine-sensitive store in a sub-population of human SH-SY5Y neuroblastoma cells[J]. Cell Calcium, 2005,38(2):111-120
doi: 10.1016/j.ceca.2005.06.001
[16] Thebault S, Flourakis M, Vanoverberghe K, Vandermoere F, Roudbaraki M, Lehen’kyi V, Slomianny C, Beck B, Mariot P, Bonnal JL, Mauroy B, Shuba Y, Capiod T, Skryma R, Prevarskaya N . Differential role of transient receptor potential channels in Ca2+ entry and proliferation of prostate cancer epithelial cells[J]. Cancer Res, 2006,66(4):2038-2047
doi: 10.1158/0008-5472.CAN-05-0376
[17] Berridge MJ . Smooth muscle cell calcium activation mechanisms[J]. J Physiol, 2008,586(Pt 21):5047-5061
doi: 10.1113/jphysiol.2008.160440 pmid: 18787034
[18] Cohen RA, Vanhoutte PM . Endothelium-dependent hyperpolarization. Beyond nitric oxide and cyclic GMP[J]. Circulation, 1995,92(11):3337-3349
doi: 10.1161/01.CIR.92.11.3337
[19] Chambliss KL, Shaul PW . Estrogen modulation of endothelial nitric oxide synthase[J]. Endocr Rev, 2002,23(5):665-686
doi: 10.1210/er.2001-0045
[20] Simoncini T, Hafezi-Moghadam A, Brazil DP, Ley K, Chin WW, Liao JK . Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase[J]. Nature, 2000,407(6803):538-541
doi: 10.1038/35035131
[1] Brody Slostad, Tejinder Khalsa, Kathleen Young, Hildalicia Guerra, Anjali Bhagra. A case-based approach to integrative medicine for cardiovascular disease prevention. Journal of Integrative Medicine, 2020, 18(2): 159-162.
[2] Noriko Shinjyo, James Parkinson, Jimmy Bell, Tatsuro Katsuno, Annie Bligh. Berberine for prevention of dementia associated with diabetes and its comorbidities: A systematic review. Journal of Integrative Medicine, 2020, 18(2): 125-151.
[3] Sherman Gu, Arthur Yin Fan. Controversial conclusions from two randomized controlled trials for acupuncture's effects on polycystic ovary syndrome or in vitro fertilization support. Journal of Integrative Medicine, 2020, 18(2): 89-91.
[4] Sana Zaki, Nasreen Jahan, Mohd Kalim, Ghausia Islam. In vitro antilithiatic activity of the hydro-alcoholic extract of Cinnamomum zeylanicum Blume bark on calcium oxalate crystallization. Journal of Integrative Medicine, 2019, 17(4): 273-281.
[5] Stefania Lamponi, Anna Maria Aloisi, Claudia Bonechi, Marco Consumi, Alessandro Donati, Gemma Leone, Claudio Rossi, Gabriella Tamasi, Luana Ghiandai, Ersilia Ferrini, Paolo Fiorenzani, Ilaria Ceccarelli, Agnese Magnani. Evaluation of in vitro cell and blood compatibility and in vivo analgesic activity of plant-derived dietary supplements. Journal of Integrative Medicine, 2019, 17(3): 213-220.
[6] Rohitash Jamwal. Bioavailable curcumin formulations: A review of pharmacokinetic studies in healthy volunteers. Journal of Integrative Medicine, 2018, 16(6): 367-374.
[7] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Thongchai Koobkokkruad, Chutichot Pattamadilok, Nontima Vardhanabhuti . Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2018, 16(6): 427-434.
[8] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[9] Shohini Chakraborty, Nashra Afaq, Neelam Singh, Sukanta Majumdar. Antimicrobial activity of Cannabis sativaThuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of Integrative Medicine, 2018, 16(5): 350-357.
[10] Bruno José Martins Da Silva, Amanda Anastácia Pinto Hage, Edilene Oliveira Silva, Ana Paula Drummond Rodrigues. Medicinal plants from the Brazilian Amazonian region and their antileishmanial activity: A review. Journal of Integrative Medicine, 2018, 16(4): 211-222.
[11] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[12] Sahar Dehdari, Homa Hajimehdipoor, Somayeh Esmaeili, Rasool Choopani, Seyed Alireza Mortazavi. Traditional and modern aspects of hemorrhoid treatment in Iran: A review. Journal of Integrative Medicine, 2018, 16(2): 90-98.
[13] Chun-song Hu, Tengiz Tkebuchava. SEEDi1.0-3.0 strategies for major noncommunicable diseases in China. Journal of Integrative Medicine, 2017, 15(4): 265-269.
[14] Chen Fu, Nevin Lianwen Zhang, Bao-xin Chen, Zhou Rong Chen, Xiang Lan Jin, Rong-juan Guo, Zhi-gang Chen, Yun-ling Zhang. Identification and classification of traditional Chinese medicine syndrome types among senior patients with vascular mild cognitive impairment using latent tree analysis. Journal of Integrative Medicine, 2017, 15(3): 186-200.
[15] Nisha Panth, Keshav Raj Paudel, Rajendra Karki. Phytochemical profile and biological activity of Juglans regia. Journal of Integrative Medicine, 2016, 14(5): 359-373.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[2] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[7] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[8] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[9] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[10] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040