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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (3): 223-227.doi: 10.3736/jcim20090305

• Original Experimental Research • Previous Articles     Next Articles

Effects of sophoricoside and genistein on biological characteristics of osteoblasts

Y Xu,WZ Chen,N Du   

  • Received:2008-04-07 Accepted:2008-12-26 Online:2009-03-20 Published:2009-03-15

Objective

 To observe the effects of sophoricoside and genistein on the biological characteristics of osteoblasts.
Methods

Osteoblasts were isolated from parietal bone of the newborn SD rats (less than 24 hours) and cultured in minimum essential medium (MEM). The effects of different concentrations of sophoricoside and genistein (10, 1 and 0.1 μmol/L) on the bone formation of osteoblasts in vitro were observed by cell proliferation rate. Differentiation of osteoblasts was evaluated by detecting the activity of alkaline phosphatase (ALP) using 4-nitrophenyl phosphate disodium salt (PNPP) method and by measuring the mineralized nodiform dyed by alizarin bordeaux. And the activity of osteoprotegerin (OPG) promoters of human beings was tested by using gene cloning and reporter gene.
Results

Sophoricoside at 1 and 0.1 μmol/L could promote the proliferation of osteoblasts (vs control group, P<0.05). Genistein at 10 μmol/L could inhibit the cell proliferation, while genistein at 0.1 μmol/L showed promotion to cell proliferation (vs control group, P<0.05). The activity of ALP of osteoblasts was increased by sophoricoside at concentrations from 0.1 μmol/L to 10 μmol/L, but genistein only at concentration of 0.1 μmol/L could make it. The total areas of mineralization of the osteoblasts in 10, 1 and 0.1 μmol/L sophoricoside groups increased by 73%, 138.6% and 114.3% as compared with the control group, respectively, but those in different concentrations of genistein groups decreased. The activity of LacZ was increased by sophoricoside at concentrations from 0.1 μmol/L to 10 μmol/L, as well as by genistein at concentration of 0.1 μmol/L.
Conclusion

Sophoricoside can stimulate the proliferation, differentiation and mineralization of osteoblasts. And it shows no inhibition to bone formation of osteoblasts compared with genistein. Sophoricoside can also increase the activity of osteoprotegerin promoter and is more effective than genistein.

Table 1

Effects of sophoricoside and genistein on proliferation and ALP activity of osteoblasts(x±s)"

Group n OD value
Proliferation of
osteoblasts
ALP activity of
osteoblasts
Control 8 0.226±0.016 0.644±0.015
Genistein
10 μmol/L 8 0.130±0.014* 0.598±0.017
1 μmol/L 8 0.248±0.018 0.662±0.023
0.1 μmol/L 8 0.378±0.032* 0.674±0.015*
Sophoricoside
10 μmol/L 8 0.231±0.017 0.670±0.025*
1 μmol/L 8 0.265±0.022* 0.673±0.026*
0.1 μmol/L 8 0.367±0.048* 0.691±0.027*
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