Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (3): 223-227.doi: 10.3736/jcim20090305

• Original Experimental Research • Previous Articles     Next Articles

Effects of sophoricoside and genistein on biological characteristics of osteoblasts

Y Xu,WZ Chen,N Du   

  • Received:2008-04-07 Accepted:2008-12-26 Online:2009-03-20 Published:2009-03-15


 To observe the effects of sophoricoside and genistein on the biological characteristics of osteoblasts.

Osteoblasts were isolated from parietal bone of the newborn SD rats (less than 24 hours) and cultured in minimum essential medium (MEM). The effects of different concentrations of sophoricoside and genistein (10, 1 and 0.1 μmol/L) on the bone formation of osteoblasts in vitro were observed by cell proliferation rate. Differentiation of osteoblasts was evaluated by detecting the activity of alkaline phosphatase (ALP) using 4-nitrophenyl phosphate disodium salt (PNPP) method and by measuring the mineralized nodiform dyed by alizarin bordeaux. And the activity of osteoprotegerin (OPG) promoters of human beings was tested by using gene cloning and reporter gene.

Sophoricoside at 1 and 0.1 μmol/L could promote the proliferation of osteoblasts (vs control group, P<0.05). Genistein at 10 μmol/L could inhibit the cell proliferation, while genistein at 0.1 μmol/L showed promotion to cell proliferation (vs control group, P<0.05). The activity of ALP of osteoblasts was increased by sophoricoside at concentrations from 0.1 μmol/L to 10 μmol/L, but genistein only at concentration of 0.1 μmol/L could make it. The total areas of mineralization of the osteoblasts in 10, 1 and 0.1 μmol/L sophoricoside groups increased by 73%, 138.6% and 114.3% as compared with the control group, respectively, but those in different concentrations of genistein groups decreased. The activity of LacZ was increased by sophoricoside at concentrations from 0.1 μmol/L to 10 μmol/L, as well as by genistein at concentration of 0.1 μmol/L.

Sophoricoside can stimulate the proliferation, differentiation and mineralization of osteoblasts. And it shows no inhibition to bone formation of osteoblasts compared with genistein. Sophoricoside can also increase the activity of osteoprotegerin promoter and is more effective than genistein.

Table 1

Effects of sophoricoside and genistein on proliferation and ALP activity of osteoblasts(x±s)"

Group n OD value
Proliferation of
ALP activity of
Control 8 0.226±0.016 0.644±0.015
10 μmol/L 8 0.130±0.014* 0.598±0.017
1 μmol/L 8 0.248±0.018 0.662±0.023
0.1 μmol/L 8 0.378±0.032* 0.674±0.015*
10 μmol/L 8 0.231±0.017 0.670±0.025*
1 μmol/L 8 0.265±0.022* 0.673±0.026*
0.1 μmol/L 8 0.367±0.048* 0.691±0.027*
[1] Coxam V . Phyto-oestrogens and bone health[J]. Proc Nutr Soc, 2008,67(2):184-195
doi: 10.1017/S0029665108007027
[2] Du N, Xu Y, Chen WZ, Zhang FH . Effect of sophoricoside on histomorphology of bone in ovariectomized rats[J]. J Chin Integr Med, 2003,1(1):44-46
杜宁, 许勇, 陈伟珍, 张风华 . 槐甙对去卵巢大鼠骨量丢失的预防作用[J]. 中西医结合学报, 2003,1(1):44-46
[3] Du N, Chen WZ, Xu Y, Liu T, Ye JQ, Fan YH . Observation on therapeutic effect of sophoricoside capsule for postmenopausal osteoporosis[J]. Zhongguo Lin Chuang Kang Fu, 2003,7(30):4122-4123
杜宁, 陈伟珍, 许勇, 刘涛, 叶剑璆, 范蕴华 . 槐甙胶囊防治绝经后骨质疏松症的疗效观察[J]. 中国临床康复, 2003,7(30):4122-4123
[4] Hamel P, Abed E, Brissette L, Moreau R . Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells[J]. Am J Physiol Cell Physiol, 2008,294(4):C1021-C1033
doi: 10.1152/ajpcell.00361.2007
[5] Ehrlich LA, Chung HY, Ghobrial I, Choi SJ, Morandi F, Colla S, Rizzoli V, Roodman GD, Giuliani N . IL-3 is a potential inhibitor of osteoblast differentiation in multiple myeloma[J]. Blood, 2005,106(4):1407-1414
doi: 10.1182/blood-2005-03-1080
[6] Wu J, Chen HQ, Li L, Wu WC, Sung KL . Influence of differentsized titanium particles loading on osteo-blastic differentiation and mineralization[J]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi, 2005,22(1):30-34
吴江, 陈槐卿, 李良, 吴文超, Sung KL . 不同直径的钛颗粒负荷对成骨细胞分化和矿化能力的影响[J]. 生物医学工程学杂志, 2005,22(1):30-34
[7] Chen XW, Garner SC, Anderson JJ . Isoflavones regulate interleukin-6 and osteoprotegerin synjournal during osteoblast cell differentiation via an estrogen-receptor-dependent pathway[J]. Biochem Biophys Res Commun, 2002,295(2):417-422
doi: 10.1016/S0006-291X(02)00667-8
[8] Gallagher JC . Advances in bone biology and new treatments for bone loss[J]. Maturitas, 2008,60(1):65-69
doi: 10.1016/j.maturitas.2008.04.005
[9] Yang HM, Shin HR, Bang SC, Lee KC, Hoangle TA, Lee IJ, Kim Y, Jung SH .The role of alkoxy group on the A ring of isoflavones in the inhibition of interleukin-5[J]. Arch Pharm Res, 2007,30(8):950-954
doi: 10.1007/BF02993962
[10] Morito K, Hirose T, Kinjo J, Hirakawa T, Okawa M, Nohara T, Ogawa S, Inoue S, Muramatsu M, Masamune Y . Interaction of phytoestrogens with estrogen receptors alpha and beta[J]. Biol Pharm Bull, 2001,24(4):351-356
doi: 10.1248/bpb.24.351
No related articles found!
Full text



[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239