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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 565-569.doi: 10.3736/jcim20110516

• Original Experimental Research • Previous Articles     Next Articles

Thread-dragging therapy in treatment of a rat model of infected subcutaneous fistula

Chen Wang1, Jin-gen Lu1, Hao-qiang Yin2, Yong-qing Cao1()   

  1. 1. Department of Anorectal Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Department of Medical Imaging, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2011-01-15 Accepted:2011-02-21 Online:2011-05-20 Published:2011-05-15
  • Contact: Cao Yong-qing E-mail:caoyq88@sh163.net

Objective: This study was to develop an experimental rat model of subcutaneous fistula induced by bacteria infection and treat it with thread-dragging therapy.
Methods: A spring-gauze was surgically implanted into 24 male Sprague-Dawley rats’ dorsal muscles, respectively, then mixed Staphylococcus aureus and Escherichia coli were injected into the spring-gauze. After 45 d, all the spring-gauzes were removed from the rats under anesthesia. X-ray and ultrasound were performed to assess the fistula tract. Each rat was examined by a fistula probe and four rats were randomly selected for histological assessment. The residual rats were randomly divided into two groups. Group A was treated with thread-dragging therapy, and group B was treated with fistulotomy. The healing time and body weight of the two groups were compared.
Results: On the 45th day of the spring-gauze implantation, imaging assessment proved a tract with two openings in all rats and histological results proved that lumen and surrounding granulation tissue with epithelium were similar to the features of fistula in human beings. The mean healing time of rats in group A was shorter than that of the rats in group B (P<0.01). The body weight of rats in group A was heavier than that of the rats in group B on the 7th and 14th day after surgery, respectively (P<0.05).
Conclusion: A rat model of subcutaneous fistula induced by mixed Staphylococcus aureus and Escherichia coli infection was successfully established. Traditional Chinese medicine thread-dragging therapy is less invasive and safer than fistulotomy.

Key words: subcutaneous fistula, thread-dragging therapy, traditional Chinese medicine, models, animal

Figure 1

Spring-gauze was implanted beneath the rat’s dorsal muscle"

Figure 2

Thread-dragging therapy"

Figure 3

Fistulotomy therapy"

Figure 4

Little yellow pus can be found at the surface of spring-gauze after 45 d of modeling"

Figure 5

Histology of fistulas on the 45th day tested by hematoxylin and eosin staining (Light microscopy,×50) The arrows point the lumen of fistula track and the thickness of inner layer was uneven."

Figure 6

Two tiny scars on the rat’s skin with thread-dragging therapy"

Figure 7

Severe scar on the rat’s skin with fistulotomy"

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[1] Wolff BG, Fleshman JW, Beck DE, Pemberton JH, Wexner SD . The ASCRS textbook of colon and rectal surgery[M]. New York: Springer, 2007: 198-214
[2] Buchanan GN, Sibbons P, Osborn M, Bartram CI, Ansari T, Halligan S, Cohen CR . Experimental model of fistula-in-ano[J]. Dis Colon Rectum, 2005,48(2):353-358
doi: 10.1007/s10350-004-0769-7 pmid: 15714247
[3] Zhao BM, Zhang SX. Study on diseases of large intestine and anus[M]. Shanghai: Second Military Medical University Press, 2004: 553
赵宝明, 张书信 . 大肠肛门病学[M]. 上海: 第二军医大学出版社, 2004: 553
[4] Lu JG, Que HF, Chen HF, Cao YQ, Wang YF, Wang C, Yao YB . Superiority of thread-dragging therapy in treatment of refractory sinus tracts or fistulae[J]. J Chin Integr Med, 2008,6(10):991-994
陆金根, 阙华发, 陈红风, 曹永清, 王云飞, 王琛, 姚一博 . 拖线疗法治疗难愈性窦瘘的优势[J]. 中西医结合学报, 2008,6(10):991-994
[5] Johnson EK, Gaw JU, Armstrong DN . Efficacy of anal fistula plug vs. fibrin glue in closure of anorectal fistulas[J]. Dis Colon Rectum, 2006,49(3):371-376
doi: 10.1007/s10350-005-0288-1 pmid: 16421664
[6] Lu JG, Cao YQ, He CM, Guo XT, Huang HX, Yi J, Xiao LX, Xu YM, Din M, Pan YB, Wang C . Clinical research of thread-dragging through fistula method in treating patients with simple anorectal fistula[J]. J Chin Integr Med, 2006,4(2):140-146
陆金根, 曹永清, 何春梅, 郭修田, 黄鸿翔, 易进, 肖立新, 徐昱旻, 丁敏, 潘一滨, 王琛 . 隧道式拖线术治疗单纯性肛瘘的临床研究[J]. 中西医结合学报, 2006,4(2):140-146
[7] Fitch MT, Manthey DE, McGinnis HD, Nicks BA, Pariyadath M .A skin abscess model for teaching incision and drainage procedures[J]. BMC Med Educ, 2008,8(1):38
doi: 10.1186/1472-6920-8-38 pmid: 2453116
[8] Grace RH, Harper IA, Thompson RG . Anorectal sepsis: microbiology in relation to fistula-in-ano[J]. Br J Surg, 1982,69(7):401-403
doi: 10.1002/bjs.1800690715 pmid: 7104611
[9] Lunniss PJ, Faris B, Rees HC, Heard S, Phillips RK . Histological and microbiological assessment of the role of microorganisms in chronic anal fistula[J]. Br J Surg, 1993,80(8):1072
doi: 10.1002/(ISSN)1365-2168
[10] Bao Y, Li YS, Li JS . A laboratory model of intraabdominal infections by intestinal fistula[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2004,21(1):99-100
doi: 10.3760/j.issn:1001-9030.2004.01.041
鲍扬, 李幼生, 黎介寿 . 一种新型腹腔感染动物模型的建立[J]. 中华实验外科杂志, 2004,21(1):99-100
doi: 10.3760/j.issn:1001-9030.2004.01.041
[11] Seow-Choen F, Hay AJ, Heard S, Phillips RK . Bacteriology of anal fistulae[J]. Br J Surg, 1992,79(1):27-28
doi: 10.1002/bjs.1800790107 pmid: 12587233
[12] Harding Rains AJ, Mann CV . Bailey and Love’s short practice of surgery[M]. London: H.K. Lewis, 1998,( 20th ed):116-117
[13] Lunniss PJ, Sheffield JP, Talbot IC, Thomson JP, Phillips RK . Persistence of idiopathic anal fistula may be related to epithelialization[J]. Br J Surg, 1995,82(1):32-33
doi: 10.1002/bjs.1800820112 pmid: 7881949
[14] Bataille F, Klebl F, Rümmele P, Schroeder J, Farkas S, Wild PJ, Fürst A, Hofstadter F, Scholmerich J, Herfarth H, Rogler G . Morphological characterisation of Crohn’s disease fistulae[J]. Gut, 2004,53(9):1314-1321
doi: 10.1136/gut.2003.038208 pmid: 1774207
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