Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (1): 39-41.doi: 10.3736/jcim20030116

Previous Articles     Next Articles

Study of the mechanism of preventing proteinuria by Yishen Gujing Decoction (YSGJD) in early diabetic nephropathy

Xiao-jing Zhou, Jian-xiong Shen, Lan Huang   

  1. Department of Nephrology, Yueyang Hospital of Integrated Chinese and Western Medicine,Shanghai
  • Received:2003-02-15 Online:2003-03-20 Published:2018-10-19


To study the mechanism of preventing proteinuria by traditional Chinese medicine YSGJD in early diabetic nephropathy (DN) in rats.


The male Sprague-Dawley (SD) rats were randomly divided into three groups: normal control, untreated control and treated groups. Experimental diabetes mellitus (DM) was induced in untreated control and treated rats with streptozotocin (STZ), and the normal group was given equal buffer solution. The treated rats were given YSGJD daily. The other two groups were given running water. Eight weeks later,the rats were raised in metabolic cages for 24 hours. Then, the 24 h urinary protein, serum glucose and nitric oxide (NO) were measured. In the renal cortex, the levels of iNOS mRNA and cGMP were determined by reverse transcription-polymerase chain reaction (RT-PCR) and radioimmunoassay separately.


Compared with normal control, in DN rats, the 24 h urine protein, the levels of renal cortex iNOS mRNA and cGMP were increased significantly (P<0.01). All above in treated group were reduced than in untreated control (P<0.05). The serum glucose and NO were increased in DN rats than in normal control (P<0.01), and had a little decrease in the treated group, with no significance between untreated and treated groups.


This recipe can diminish the urine protein in early DN without the significant decrease of serum sugar, can correct the abnormal levels of iNOS mRNA and cGMP in renal cortex. Interfering with the function of NO may be one of its mechanisms.

Key words: diabetic nephropathy, proteinuria, nitric oxide, therapy, TCM

CLC Number: 

  • R587.24


分组 例数 血糖(mmol/L) 24 h尿蛋白总量(mg/24 h) 血NO(μmol/L) 肾cGMP(pmol/g) 肾iNOS mRNA
正常组 11 5.4±0.7 17.4±5.5 9.7±8.1 7.9±1.3 0.15±0.05
对照组 7 19.5±2.1** 74.5±30.3** 18.0±14.5** 12.4±2.7** 0.54±0.19**
中药治疗组 9 16.6±5.1** 38.6±21.3 17.0±14.7** 9.5±2.1 0.32±0.13
[1] Mogensen CE. Early diabetic renal involvement nephropathy[A]. In: Alberti KGMM,Krall LP. The diabetes[M]. Vol 3. Amsterdam: Elsevier Science publishers, 1987. 306.
[2] Andersen AR, Christiansen JS, Andersen JK , et al. Diabetic nephropathy in type 1(insulin-dependent) diabetes: an epidemiological study[J]. Diabetologia, 1983,25(6):496-501
[3] Amore A, Cirina P, Mitola S , et al. Nonenzymatically glycated albumin(Amadori adducts) enhances nitric oxide synthase activity and gene expression in endothelial cells[J]. Kidney Int, 1997,51(1):27-35
doi: 10.1038/ki.1997.4
[4] 钟慈奇, 孙安阳 . 一氧化氮的生物医学(第1版)[M]. 上海: 上海医科大学出版社, 1996,( 第1版):16-17
[5] 陈雪梅, 杜晓刚, 李荣享 . 一氧化氮与肾小球疾病[J]. 国外医学·泌尿系统分册, 2000,20(5):201-203
[6] Trachtman H, Koss I, Bogart M , et al. High glucose enhances growth factor-stimulated nitric oxide production by cultured rat messengial cells[J]. Res Commun Mol Pathol Pharmacol, 1998,100(2):213-215
[7] Hostetter TH, Troy JL, Brenner BM . Glomerular hemodynamics in experimental diabetes mellitus[J]. Kidney Int, 1981,19(3):410-405
doi: 10.1038/ki.1981.33 pmid: 7241881
[8] 刘华锋, 陈孝文 . 一氧化氮与肾损伤的研究进展[J]. 国外医学·泌尿系统分册, 2000,20(5):198-200
[9] Veelken R, Hilgers KF, Hartner A , et al. Nitric oxide synthase isoforms and glomerular hyperfiltration in early diabetic nephropathy[J]. J Am Soc Nephrol, 2000,11(1):71-79
doi: 10.1046/j.1442-2042.2000.00131.x pmid: 10616842
[10] 祁忠华, 林善琰, 黄宇峰 . 黄芪改善糖尿病早期肾血流动力学异常的研究[J]. 中国糖尿病杂志, 1999,7(3):147-149
doi: 10.1016/B978-008043005-8/50012-3
[11] 祁忠华, 林善琰 . 卡托普利调节早期糖尿病大鼠肾脏诱生型一氧化氮合成酶基因表达[J]. 中华肾脏病杂志, 1996,12(6):332-335
[12] 朱又春 . 名老中医朱良春应用鬼箭羽经验[J]. 现代中医, 1997,10(3):136
[1] Tara Rajendran. Addressing the need for personalizing music therapy in integrative oncology. Journal of Integrative Medicine, 2022, 20(4): 281-283.
[2] Tara Rajendran, Martha Summa-Chadwick. The scope and potential of music therapy in stroke rehabilitation. Journal of Integrative Medicine, 2022, 20(4): 284-287.
[3] Lu Yin, Yun An, Xiao Chen, Hui-xin Yan, Tao Zhang, Xin-gang Lu, Jun-tao Yan. Local vibration therapy promotes the recovery of nerve function in rats with sciatic nerve injury. Journal of Integrative Medicine, 2022, 20(3): 265-273.
[4] Bin Zhao, Yong Chen, Shi-min Liao, Jian-ying Zheng, Shi-hua Yan, Dong-shu Zhang. Successfully treated recalcitrant atopic eczema with acupoint autohemotherapy: A case report and hypothesized mechanism of the therapy. Journal of Integrative Medicine, 2022, 20(2): 182-186.
[5] Yan-jun Lin, Kun-li Jiao, Bo Liu, Lu Fang, Shu Meng. Antiplatelet and myocardial protective effect of shexiang tongxin dropping pill in patients undergoing percutaneous coronary intervention: A randomized controlled trial. Journal of Integrative Medicine, 2022, 20(2): 126-134.
[6] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[7] Gai Ran, Xi-lin Feng, Yi-lin Xie, Qing-yun Zheng, Peng-peng Guo, Ming Yang, Ying-lu Feng, Chen Ling, Li-qing Zhu, Chen Zhong. The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy. Journal of Integrative Medicine, 2021, 19(6): 515-525.
[8] Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[9] Zahra Karimzadeh, Mansooreh Azizzadeh Forouzi, Haleh Tajadini, Mehdi Ahmadinejad, Callista Roy, Mahlagha Dehghan. Effects of lavender and Citrus aurantium on pain of conscious intensive care unit patients: A parallel randomized placebo-controlled trial. Journal of Integrative Medicine, 2021, 19(4): 333-339.
[10] Ning Zhang, Xiao-he Xiao. Integrative medicine in the era of cancer immunotherapy: Challenges and opportunities. Journal of Integrative Medicine, 2021, 19(4): 291-294.
[11] Riska Siregar, Aris Setyawan, Syahruramdhani Syahruramdhani. A model to standardize safety and quality of care for cupping therapy. Journal of Integrative Medicine, 2021, 19(4): 327-332.
[12] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[13] Nurul Afiedia Roslim, Aryati Ahmad, Mardiana Mansor, Myat Moe Thwe Aung, Farrahdilla Hamzah, Haszalina Hassan, Pei Lin Lua. Hypnotherapy for overweight and obese patients: A narrative review. Journal of Integrative Medicine, 2021, 19(1): 1-5.
[14] Swatantra Kumar, Vimal K Maurya, Debadatta Nayak, Anil Khurana, Raj K Manchanda, Srinivasulu Gadugu, Madan LB Bhatt, Shailendra K Saxena. Calcarea carbonica treatment rescues lipopolysaccharide-induced inflammatory response in human mononuclear cells via downregulation of inducible cyclooxygenase pathway. Journal of Integrative Medicine, 2020, 18(5): 441-449.
[15] Johannes Loeser, Brigitte Layer, Christopher Plata, Klaus-Maria Perrar, Tim Hucho, Rebecca Kulbida. Hirudotherapy attenuates arthritic pain in patients with various chronic pain syndromes: A retrospective analysis. Journal of Integrative Medicine, 2020, 18(5): 425-433.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228