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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (1): 42-43.doi: 10.3736/jcim20030117

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Study on Realgar inducing apoptosis in T lymphocytic cell line CEM

Chen Zhang(), Shi-lin Huang, Yang Xiang, Ai-xia Guo   

  1. TCM Hematology Institute of People’s Liberation Army, Dalian,Liaoning Province 116021,China
  • Received:2003-03-04 Online:2003-03-20 Published:2018-10-19


To study the Realgar induced T lymphocytic leukemia cell line CEM apoptosis in vitro.


CEM cells were incubated with Realgar. Cell proliferation inhibition was determined by MTT. Cell cycle, apoptosis, Apo2.7 and Fas were measured by cytometer.


Realgar inhibited the proliferation of CEM cell line. The cells treated with Realgar showed a Sub-G0/G1 apoptotic peak in DNA distribution histogram, increment of Apo2.7 protein expression, and arrested cells in G2/M phase, but ineffectiveness on Fas expression.


The Realgar can induce CEM cell apoptosis in vitro.

Key words: Realgar, leukemia, apoptosis

CLC Number: 

  • R733.7


药物浓度(mg/L) G0/G1 S G2/M 凋亡率
对照组 62.2 27.6 10.2 3.6
15 58.1 23.3 18.6 9.1
30 59.2 20.7 20.1 12.6
60 53.5 21.1 25.3 21.5


药物浓度(mg/L) Apo2.7 Fas
对照组 2.83±2.03 15.10±2.49
15 11.07±1.89* 13.10±5.31
30 18.72±8.54* 12.50±1.85
60 30.70±9.43* 15.70±3.45
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