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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 115-116.doi: 10.3736/jcim20040211

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Effect of hesperidin and rutin on oxidative modification of high density lipoprotein in vitro

Qin-shan Li1, Gui-yu Lou2, Min-zhang Qian1   

  1. 1. Deparment of Biochemistry, Zunyi Medical College, Zunyi,Guizhou Province 563003,China
    2. Deparment of Biochemistry, Third Military Medical University,Chongqing 400038, China
  • Online:2004-03-20 Published:2018-10-25

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Objective: To study the effect of hesperidin and rutin on oxidative modification of high density lipoprotein (HDL) in vitro.Methods: HDL was isolated from healthy human plasma by sequential ultracentrifugation, and was oxidized by copper ions. The inhibitory effects of hesperidin and rutin on HDL oxidative modification were valued by the formation of malondialdehyde (MDA). Results: Hesperidin and rutin significantly inhibited copper-induced oxidation of HDL in a dose-dependent manner.Conclusion: Both hesperidin and rutin can prevent HDL from copper-induced oxidative modification in vitro. This result suggests that they might have antiatherogenic effect.

Key words: hesperidin, rutin, high density lipoproteins, atherosclerosis

CLC Number: 

  • R285

Fig. 1

Agarose gel electrophoresis of HDL samples^1：ox-HDL; 2：HDL; 3：Normal serum"

Tab 1

Effect of hesperidin on MDA in Cu2+ induced oxidation of HDL (ヌ±S)"

Group n MDA(μmol/L)
HDL 3 4.36±2.23
ox-HDL 3 29.54±2.27**
Hesperidin(3 mg/L) 3 25.00±0.08△
Hesperidin(9 mg/L) 3 17.17±1.16△△
Hesperidin(27 mg/L) 3 12.12±1.31△△
Hesperidin(80 mg/L) 3 6.06±1.53△△
Hesperidin(160 mg/L) 3 4.51±2.19△△

Tab 2

Effect of rutin on MDA in Cu2+ induced oxidation of HDL (ヌ±S)"

Group n MDA（μmol/L）
HDL 3 4.36±2.23
ox-HDL 3 29.54±2.27**
Hesperidin(3 mg/L) 3 27.27±2.18
Hesperidin(9 mg/L) 3 19.69±1.36 △△
Hesperidin(27 mg/L) 3 15.15±2.62 △△
Hesperidin(80 mg/L) 3 9.85±1.39 △△
Hesperidin(160 mg/L) 3 6.06±1.25△△
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