SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (6): 667-673.doi: 10.3736/jcim20120611

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chinese herbal medicine Shoushen Granule on telomere length and telomerase activity of peripheral white blood cells and vascular cells in rats with atherosclerosis

Chuan Chen(), Hui-ying Chi, Zhi-hua Yu, Jiu-lin Chen#br#   

  1. Shanghai Geriatric Institute of Chinese Medicine, Shanghai 200032, China
  • Received:2012-02-06 Accepted:2012-04-11 Online:2012-06-20 Published:2018-06-15
  • Contact: Chuan Chen E-mail:ch9453@126.com

RichHTML

3

PDF (PC)

86

Like

Objective: To observe the effects of Shoushen Granule, a compound traditional Chinese herbal medicine, on telomere length and telomerase activity in peripheral leukocytes and vascular cells, artery wall lesions and blood lipid in a Sprague-Dawley (SD) rat model of atherosclerosis.
Methods: Forty SD rats were randomly divided into normal control group, model group, Shoushen Granule group and Western medicine group with 10 in each group. The rat model of atherosclerosis was established by high-fat diet and vitamin D3 loading. The model group was given gastric perfusion of double distilled water; The Shoushen Granule group and the Western medicine group were respectively given gastric perfusion of Shoushen Granule and pravastatin. After 12 weeks, pathological changes of abdominal aorta were determined by hematoxylin and eosin staining. Biochemical colorimetric method was used to detect the contents of total cholesterol (TC), triacylglycerol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol (LDL-C) in serum of the rats. Telomere length and telomerase activity in peripheral leukocytes and vascular cells of the rats were tested by quantitative real-time polymerase chain reaction method.
Results: When compared with the model group, atherosclerosis lesions of the arterial wall were significantly improved in the Shoushen Granule group. In addition, both TC and LDL-C levels in the Shoushen Granule group were decreased significantly compared with the model group (P<0.01). Besides, not only telomerase activity but also telomere length in peripheral leukocytes (P<0.01) and vascular cells (P<0.05) were increased significantly as compared to those in the model group. However, there was no significant difference between the Shoushen Granule group and the normal control group (P>0.05).
Conclusion: Shoushen Granule improves the atherosclerosis lesions in rats, and the mechanism may be related to regulating telomere length and telomerase activity.

Key words: drugs, Chinese herbal, atherosclerosis, telomeres, telomerase, dyslipidemias, rats

Table 1

The primer sequences of telomere and telomerase"

Gene Primer sequence (5′-3′)
Telomere Forward: CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT
Reverse: GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT
Telomerase Forward: CAGCTCCCATTTCATCAGCAA
Reverse: GCGACATCCCTGCGTTCTT
Internal control gene Forward: CAGCAAGTGGGAAGGTGTAATCC
Reverse: CCCATTCTATCATCAACGGGTACAA

Figure 1

Abdominal aorta of rats observed by hematoxylin and eosin staining (Light microscopy, ×100) A: Normal control group; B: Model group; C: Pravastatin group; D: Shoushen Granule group."

Table 2

TC, TAG, LDL-C, HDL-C levels in serum and ratio of TC/HDL-C in different groups (x±s)"

Group n TC (mmol/L) TAG (mmol/L) LDL-C (mmol/L) HDL-C (mmol/L) TC/HDL-C
Normal control 6 1.842±0.181 0.533±0.158 0.497±0.172 0.946±0.017 1.945±0.162
Model 6 6.320±0.826** 0.312±0.050** 4.832±1.092** 0.905±0.020** 6.977±0.884**
Pravastatin 6 4.464±1.531△ 0.427±0.061 3.259±1.288△ 0.880±0.022 5.081±1.762△
Shoushen Granule 6 3.754±0.681△△ 0.237±0.026 1.689±0.717△△▲ 0.867±0.021 4.344±0.873△△

Table 3

Telomere length and telomerase activity in different groups (x±s)"

Group n Telomere length Telomerase activity
Vascular cells Peripheral leukocytes Vascular cells Peripheral leukocytes
Normal control 6 3.848±0.933 0.940±0.181 16.988±3.191 0.874±0.386
Model 6 2.586±0.199* 0.514±0.058** 11.685±2.319* 1.026±0.283
Pravastatin 6 7.355±1.005△△** 2.374±0.344**△△ 15.400±1.782△ 2.203±0.614*△
Shoushen Granule 6 3.083±1.627 1.176±0.194△△ 17.034±1.973△△ 2.358±0.530**△△
[1] Nzietchueng R, Elfarra M, Nloga J, Labat C, Carteaux JP, Maureira P, Lacolley P, Villemot JP, Benetos A . Telomere length in vascular tissues from patients with atherosclerotic disease[J]. J Nutr Health Aging, 2011,15(2):153-156
doi: 10.1007/s12603-011-0029-1 pmid: 21365170
[2] Kroenke CH, Pletcher MJ, Lin J, Blackburn E, Adler N, Matthews K, Epel E . Telomerase, telomere length, and coronary artery calcium in black and white men in the CARDIA study[J]. Atherosclerosis, 2012,220(2):506-512
doi: 10.1016/j.atherosclerosis.2011.10.041
[3] Calvert PA, Liew TV, Gorenne I, Clarke M, Costopoulos C, Obaid DR , O’Sullivan M, Shapiro LM, McNab DC, Densem CG, Schofield PM, Braganza D, Clarke SC, Ray KK, West NE, Bennett MR . Leukocyte telomere length is associated with high-risk plaques on virtual histology intravascular ultrasound and increased proinflammatory activity[J]. Arterioscler Thromb Vasc Biol, 2011,31(9):2157-2164
doi: 10.1161/ATVBAHA.111.229237
[4] Lu JB, Zhou RQ, Xu PC, Yu ZH, Wang J, Zhao DM, Cao DF, Fang J, Lin SM . Effect of different components in meridian-strengthening 981 on atherosclerosis in rabbits[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2001,7(10):33-36
doi: 10.3969/j.issn.1006-3250.2001.10.014
陆金宝, 周如倩, 徐品初, 郁志华, 王健, 赵德敏, 曹棣芳, 方军, 林水淼 . 健脉981拆方治疗家兔动脉粥样硬化的实验研究[J]. 中国中医基础医学杂志, 2001,7(10):33-36
doi: 10.3969/j.issn.1006-3250.2001.10.014
[5] Lin SM, Lu JB . Experimental study on “meridian-strengthening 981” in eliminating atherosclerotic plaque in rabbits[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 1999,13(4):43-46
林水淼, 陆金宝 . 健脉981对消退家兔动脉粥样硬化斑块的实验研究[J]. 上海中医药大学学报, 1999,13(4):43-46
[6] Lu JB, Lin SM, Cao DF . Experimental study on regressive effect of reinforcing kidney and replenishing qi prescription for atherosclerosis plaque in rabbits[J]. Zhongguo Lao Nian Xue Za Zhi, 2002,22(9):385-387
陆金宝, 林水淼, 曹棣芳 . 补肾益气方对消退兔动脉粥样硬化斑块作用的实验研究[J]. 中国老年学杂志, 2002,22(9):385-387
[7] Yang PY, Rui YC, Jiao YB . Establishment of experimental atherosclerosis model in rats[J]. Di Er Jun Yi Da Xue Xue Bao, 2003,24(7):802-804
doi: 10.3321/j.issn:0258-879X.2003.07.031
杨鹏远, 芮耀诚, 焦亚斌 . 动脉粥样硬化大鼠实验模型的建立[J]. 第二军医大学学报, 2003,24(7):802-804
doi: 10.3321/j.issn:0258-879X.2003.07.031
[8] Xu SY, Bian RL, Chen X. Methodology of pharmacological experiment. 3rd ed[M]. Beijing: People’s Medical Publishing Hourse, 2001: 1861
徐叔云, 卞如濂, 陈修 . 药理实验方法学. 第3版[M]. 北京: 人民卫生出版社, 2001: 1861
[9] Lei DZ, Han QH . The application of the ratio of TC/HDL-C in diagnosis of cardiovascular disease[J]. Zhong Xi Yi Jie He Xin Nao Xue Guan Bing Za Zhi, 2009,7(2):235-236
雷大洲, 韩清华 . TC/HDL-C比值在冠心病诊断中的应用[J]. 中西医结合心脑血管病杂志, 2009,7(2):235-236
[10] Chen JZ, Zhang FR, Zhu JH, Zhu JH, Xie XD, Sun J, Shang YP, Guo XG . Effects of statins on number and activity of endothelial progenitor cells from peripheral blood[J]. Zhonghua Xin Xue Guan Bing Za Zhi, 2004,32(4):346-350
陈君柱, 张芙荣, 朱建华, 朱军慧, 谢旭东, 孙坚, 尚云鹏, 郭晓纲 . 他汀类药物对外周血内皮祖细胞数量和功能的影响[J]. 中华心血管病杂志, 2004,32(4):346-350
[11] Qin JM, Li JJ . Anti-inflammatory effect of statins[J]. Zhongguo Dong Mai Ying Hua Za Zhi, 2004,12(3):363-366
秦晋梅, 李建军 . 他汀类药物的抗炎作用[J]. 中国动脉硬化杂志, 2004,12(3):363-366
[12] Li YM, Chen HF, Yang YZ . Harmonized Clinical Guidelines on Prevention of Atherosclerotic Vascular Disease(Summary) announced by International Atherosclerosis Society[J]. Zhongguo Dong Mai Ying Hua Za Zhi, 2003,11(5):484-492
李悦梅, 陈慧芳, 杨永宗 . 国际动脉粥样硬化学会: 预防动脉粥样硬化性心血管疾病临床指南(摘要)[J]. 中国动脉硬化杂志, 2003,11(5):484-492
[13] Bennett MR, Littlewood TD, Schwartz SM, Weissberg PL . Increased sensitivity of human vascular smooth muscle cells from atherosclerotic plaques to p53-mediated apoptosis[J]. Circ Res, 1997,81(4):591-599
doi: 10.1161/01.RES.81.4.591
[14] Benetos A, Waeber B, Izzo J, Mitchell G, Resnick L, Asmar R, Safar M . Influence of age, risk factors, and cardiovascular and renal disease on arterial stiffness: clinical applications[J]. Am J Hypertens, 2002,15(12):1101-1108
doi: 10.1016/S0895-7061(02)03029-7
[15] Matthews C, Gorenne I, Scott S, Figg N, Kirkpatrick P, Ritchie A, Goddard M, Bennett M . Vascular smooth muscle cells undergo telomere-based senescence in human atherosclerosis: effects of telomerase and oxidative stress[J]. Circ Res, 2006,99(2):156-164
doi: 10.1161/01.RES.0000233315.38086.bc
[16] Minamino T, Miyauchi H, Yoshida T, Komuro I . Endothelial cell senescence in human atherosclerosis: role of telomeres in endothelial dysfunction[J]. J Cardiol, 2003,41(1):39-40
[17] Fitzpatrick AL, Kronmal RA, Gardner JP, Psaty BM, Jenny NS, Tracy RP, Walston J, Kimura M, Aviv A . Leukocyte telomere length and cardiovascular disease in the cardiovascular health study[J]. Am J Epidemiol, 2007,165(1):14-21
doi: 10.1093/aje/kwj346 pmid: 17043079
[18] Samani NJ, Boultby R, Butler R, Thompson JR, Goodall AH . Telomere shortening in atherosclerosis[J]. Lancet, 2001,358(9280):472-473
doi: 10.1016/S0140-6736(01)05633-1
[19] Zhong YC, Luo PL, Jin GE, Yang HL, Han SF, Ge RL . Real-time quantitative PCR in determination of telomere length[J]. Lin Chuang He Shi Yan Yi Xue Za Zhi, 2008,7(4):14-16
doi: 10.3969/j.issn.1671-4695.2008.04.007
钟英成, 罗朋立, 靳国恩, 杨慧莲, 韩淑芬, 格日力 . 荧光实时定量PCR检测端粒长度[J]. 临床和实验医学杂志, 2008,7(4):14-16
doi: 10.3969/j.issn.1671-4695.2008.04.007
[20] Friedrich U, Griese E, Schwab M, Fritz P, Thon K, Klotz U . Telomere length in different tissues of elderly patients[J]. Mech Ageing Dev, 2000,119(3):89-99
doi: 10.1016/S0047-6374(00)00173-1 pmid: 11080530
[1] Shu-yi Chen, Qun-wei Chen, Liu-mei Shou, Hong Pan, Shan-ming Ruan, Zhe-hao Liang, Qi-jin Shu. Stevens-Johnson syndrome/toxic epidermal necrolysis successfully treated with Chinese herbal medicine Pi-Yan-Ning: A case report. Journal of Integrative Medicine, 2021, 19(6): 555-560.
[2] Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[3] Ya-lei Liu, Zhen-yu Zhou, Min Gao, Guang Ji, Cheng Huang, Sheng-jie Fan. Therapeutic effects of herbal formula Huangqisan on metabolic disorders via SREBF1, SCD1 and AMPK signaling pathway. Journal of Integrative Medicine, 2021, 19(2): 167-176.
[4] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[5] Qing-lan Wang, Yan-yan Tao, Hong-dong Xie, Cheng-hai Liu, Ping Liu. Fuzheng Huayu recipe, a traditional Chinese compound herbal medicine, attenuates renal interstitial fibrosis via targeting the miR-21/PTEN/AKT axis. Journal of Integrative Medicine, 2020, 18(6): 505-513.
[6] Ming-sheng Lyu, De-ying Li, Shao-zhong Zhou, Cheng-jun Ban, Jun Yan. Adult-onset Still's disease successfully treated with Chinese herbal medicine: A case report with 15-month follow-up. Journal of Integrative Medicine, 2020, 18(6): 530-534.
[7] Arthur Yin Fan, Sherman Gu, Sarah Faggert Alemi, Research Group for Evidence-based Chinese Medicine. Chinese herbal medicine for COVID-19: Current evidence with systematic review and meta-analysis. Journal of Integrative Medicine, 2020, 18(5): 385-394.
[8] Bin Lan, Jin-wen Ge, Shao-wu Cheng, Xi-long Zheng, Jun Liao, Chao He, Zheng-qing Rao, Guo-zuo Wang. Extract of Naotaifang, a compound Chinese herbal medicine, protects neuron ferroptosis induced by acute cerebral ischemia in rats. Journal of Integrative Medicine, 2020, 18(4): 344-350.
[9] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[10] Deng-hai Zhang, Kun-lun Wu, Xue Zhang, Sheng-qiong Deng, Bin Peng. In silico screening of Chinese herbal medicines with the potential to directly inhibit 2019 novel coronavirus. Journal of Integrative Medicine, 2020, 18(2): 152-158.
[11] Fatma Mohamed Hussein Shediwah, Khalid Mohammed Naji, Hussein Saleh Gumaih, Fatima A Alhadi, Amani Lutf Al-Hammami, Myrene Roselyn D'Souza. Antioxidant and antihyperlipidemic activity of Costus speciosus against atherogenic diet-induced hyperlipidemia in rabbits. Journal of Integrative Medicine, 2019, 17(3): 181-191.
[12] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[13] Carola Analía Torres, Cristina Marisel Pérez Zamora, María Beatriz Nuñez, Ana María Gonzalez. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of Integrative Medicine, 2018, 16(4): 255-262.
[14] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[15] Zahra Hajmohammadi, Mojtaba Heydari, Majid Nimrouzi, Pouya Faridi, Mohammad Javad Zibaeenezhad, Gholamhossein Ranjbar Omrani, Mesbah Shams . Rhus coriaria L. increases serum apolipoprotein-A1 and high-density lipoprotein cholesterol levels: A double-blind placebo-controlled randomized clinical trial. Journal of Integrative Medicine, 2018, 16(1): 45-50.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3