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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (12): 1233-1237.doi: 10.3736/jcim20081205

• Original Clinical Research • Previous Articles     Next Articles

Relationship between plasma protein expression profiles and states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis

Jian-nan Song1(), Jun-lian Liu1, Xiang-zhong Fang2, Yuan-hui Hu3, Yan Lei4, Xiao-hong Niu1, Gang Wu5, Bao-sheng Chen5, Ya-luan Ma1, Bing Chen1, Hong jin1   

  1. 1. Research Center of Basic Chinese Medicine, Institute of Basic Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700, China
    2. Department of Probability and Statistics, School of Mathematical Sciences, Peking University, Beijing 100871, China
    3. Department of Cardiovascular Diseases, Guang'anmen Hospital,China Academy of Chinese Medical Sciences, Beijing 100053,China
    4. Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
    5. Department of Biochemistry, Institute of Basic Medical Sciences, China Academy of Medical Sciences, Beijing 100730, China
  • Received:2008-06-19 Online:2008-12-20 Published:2008-12-15

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Objective: To investigate the relationship between the plasma biomarker proteins and the states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis.
Methods: The states of Zang-Fu organs in 146 patients with perlipidemia and atherosclerosis were diagnosed by syndrome differentiation of traditional Chinese medicine. The plasma proteins from these patients were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Differential protein profiling was established by Image Master 6.0 software, and the differential proteins were analyzed by quadrupole time of flight mass spectrometry (Q-TOF-MS). The association between the plasma biomarker proteins and the states of Zang-Fu organs was analyzed by graphical models.
Results: The biomarker proteins such as fibrinogen gamma chain, albumin and apolipoprotein AI (precursor) in discrimination of the patients with phlegm syndrome from phlegm accumulating with stagnation syndrome were correlated with the deficiency of kidney-qi, heart-qi and spleen-qi. Among the four biomarker proteins in discrimination of the patients with phlegm syndrome from blood stagnation syndrome, albumin, adrenomedullin binding protein (precursor) and haptoglobin (precursor) were correlated with the deficiency of kidney-qi and heart-qi, but complement component C4 was independent of the deficient Zang-Fu organs. The biomarker albumin was associated with the deficiency of kidney-qi, heart-qi and spleen-qi, and adrenomedullin binding protein (precursor) was correlated with the deficiency of spleen-qi in discrimination of the patients with blood stagnation syndrome from phlegm accumulating with stagnation syndrome. As the potential biomarker proteins in discrimination of the patients with non-phlegm and non-stagnation syndrome from phlegm accumulating with stagnation syndrome, the fibrinogen beta chain was related with the deficiency of kidney-qi, and apolipoprotein AI (precursor) was correlated with both the deficiency of kidney-qi and heart-qi.
Conclusion: There exists inherent correlation between the states of Zang-Fu organs and the plasma probable biomarker proteins in the patients with different phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis.

Key words: hyperlipidemias, atherosclerosis, phlegm syndrome, blood stagnation, plasma proteins, Zang-Fu organs

CLC Number: 

  • R589.21

Table 1

Frequency of the states of Zang-Fu organs in the patients with phlegm or blood stagnation syndromes"

Sydrome type n Deficiency of heart-qi Deficiency of kidney-qi Deficiency of spleen-qi
Phlegm 32 20 14 12
Blood stagnation 38 28 22 0
Phlegm accumulating with stagnation 41 22 25 19
Non-phlegm and non-stagnation 35 16 16 8

Figure 1

Relationship between the plasma biomarker proteins and the states of Zang-Fu organs in the patients with phlegm or blood stagnation syndromes A: Phlegm syndrome; B: Blood stagnation syndrome; C: Phlegm accumulating with stagnation sydrome; D: Non-phlegm and non-stagnation syndrome."

[1] Song JN, Liu DY, Niu XH , et al. Experimental study on the relationship between hyperlipidemia and phlegm of Chinese medicine[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 1995,1(1):49-51
宋剑南, 刘东远, 牛晓红 , 等. 高脂血症与中医痰浊关系的实验研究[J]. 中国中医基础医学杂志, 1995,1(1):49-51
[2] Cao HX, Zhang HM . Recognization on the attack mechanism of coronary heart disease and interelation between phlegm and stasis[J]. Zhong Yi Yao Xue Kan, 2001,19(6):544-546
doi: 10.3969/j.issn.1673-7717.2001.06.005
曹洪欣, 张华敏 . 痰瘀互结与冠心病发病机理辨识[J]. 中医药学刊, 2001,19(6):544-546
doi: 10.3969/j.issn.1673-7717.2001.06.005
[3] Fang Q, Wang ZL, Ning TH , et al. Remedial proposal of dyslipidemia[J]. Zhonghua Xin Xue Guan Bing Za Zhi, 1997,25(3):169-175
方圻, 王钟林, 宁田海 , 等. 血脂异常防治建议[J]. 中华心血管病杂志, 1997,25(3):169-175
[4] Gibbons RJ, Balady GJ, Beasley JW , et al. ACC/AHA Guidelines for Exercise Testing. A report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines(Committee on Exercise Testing)[J]. J Am Coll Cardiol, 1997,30(1):260-311
doi: 10.1016/S0735-1097(97)00150-2
[5] State Bureau of Technical Supervision. National Standards of People's Republic of China: Clinical terminology of traditional Chinese medical diagnosis and treatment[M]. Beijing: Standards Press of China,1997
国家技术监督局. 中华人民共和国国家标准·中医临床诊疗术语(第1版)[M]. 北京: 中国标准出版社,1997,(第1版)
[6] Wang X, Song JN, Fang XZ . Multivariate analysis of symptom diagnosis in traditional Chinese medicine[J]. Beijing Da Xue Xue Bao Zi Ran Ke Xue Ban Wang Luo Ban, 2007,( 4):320-325
doi: 10.3321/j.issn:0479-8023.2008.05.001
王曦, 宋剑南, 房祥忠 . 中医证候的多元统计分析及方法研究[J]. 北京大学学报 (自然科学版)网络版, 2007,( 4):320-325
doi: 10.3321/j.issn:0479-8023.2008.05.001
[7] Song JN . Views on basic research model and method for syndrome of traditional Chinese medicine[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2007,13(2):81-83
doi: 10.3969/j.issn.1006-3250.2007.02.001
宋剑南 . 论中医证候基础研究的模式和方法[J]. 中国中医基础医学杂志, 2007,13(2):81-83
doi: 10.3969/j.issn.1006-3250.2007.02.001
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