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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 117-119.doi: 10.3736/jcim20040212

• Original Experimental Research • Previous Articles     Next Articles

Preventive effect of Breviscapus Injection on chronic hypoxic myocardium injury in rats

Sun-zhong Mao1, Zhe Yan1, Yu-peng Xie2   

  1. 1. Department of Biochemistry, Wenzhou Medical College, Wenzhou,Zhejiang Province 325027,China
    2. First Hospital, Wenzhou Medical College, Wenzhou,Zhejiang Province 325027,China
  • Online:2004-03-20 Published:2018-10-25

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Objective: To study the preventive effect of Breviscapus Injection on the chronic hypoxic myocardium injury in rats and its mechanism. Methods: Transmission electron microscope and biochemical analysis were used to assay the therapeutic effect of Breviscapus Injection in rat model induced by 4 week's hypoxia. Results: The contents of malondialdehyde(MDA) and Ca 2+ in the homogenate of myocardial tissue of the hypoxic rats were significantly higher than those of the control rats. The activities of superoxide dismutase(SOD), nitric oxide synthase (NOS) in the homogenate of myocardial tissue and the plasmic NO levels of the hypoxic rats were significantly lower than those of the control rats. Breviscapus Injection reduced the contents of MDA and Ca 2+, and increased the levels of SOD, NOS and NO. Ultrastructural examination revealed that the injuries of the ultrastructure of heart in hypoxic rats were improved by treatment with Breviscapus Injection. Conclusion: Breviscapus Injection can effectively prevent and treat the hypoxia-induced myocardial damage. One of its mechanisms may relate to its adjusting NO level, anti-damaging of free radicals and inhibiting calcium overload.

Key words: Breviscapus Injection, myocardial protection, animals, laboratory

CLC Number: 

  • R542.2

Tab 1

Activities of SOD and contents of MDA and Ca2+ in homogenate of myocardial tissue (ヌ±S)"

Group n SOD(U/mg pro) MDA(μmol/g pro) Ca2+ (μmol/g pro)
Control 10 589±86 3.7±1.2 6.3±2.1
Hypoxic rats 10 433±64** 7.7±1.8** 15.3±4.4**
Breviscapus Injection 10 504±75△* 5.6±1.5△* 8.0±1.7△△

Tab 2

Activity of NOS in homogenate of myocardialtissue and plasmic NO level (ヌ±S)"

Group n NOS(U/mg pro) NO(μmol/L)
Control 10 10.18±0.49 34.3±6.5
Hypoxic rats 10 8.21±0.44** 27.6±2.1*
Breviscapus Injection 10 9.41±0.27△ 30.7±2.1

Figure 1

Ultrastructure of myocardiumA: Control rat（×17 K）; B: Hypoxic rat（×20 K）; C: Breviscapus Injection treated hypoxic rat（×20 K）"

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