大黄素抑制系膜细胞增生与周期素激酶抑制剂p27的关系

doi:10.3736/jcim20040213

Abstract

Objective: To investigate the role of p27 in the inhibition of emodin on the mesangial cell (MC) proliferation induced by tumor necrosis factor-α (TNF-α). Methods: p27 protein of MC was detected with western blotting analysis. The degree of MC proliferation was estimated through[ ³H]thymidine ([ ³H]TdR) incorporation. Different dosage of emodin (50 mg/L,100 mg/L) was added into MC stimulated by TNF-α. Results: TNF-α (200 kU/L) decreased p27 level of MC cultured in serum-free DMEM for 24 hours and increased[ ³H]TdR incorporation. Emodin increased p27 level of MC stimulated by TNF-α and decreased [ ³H]TdR incorporation. The more the emodin was added, the greater the above-mentioned effect of emodin. Conclusion: The increment of p27 level maybe play an important role in the inhibition of emodin on MC proliferation induced by TNF-α.

Key words: emodin, cyclin kinase inhibitor, tumor necrosis factor-α, mesangial cell

CLC Number:

R285.6

Xiao-bin Mei, Wei-jie Yuan, Feng-lan Zhan, Hao Wu, Xiao-ying Zhang, Ruo-lan Cui. Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-α. Journal of Chinese Integrative Medicine, 2004, 2(2): 120-122.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20040213

http://www.jcimjournal.com/EN/Y2004/V2/I2/120

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References 12

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Group	n	[³H]TdR incorporation (cpm/well)
Control	6	685±53
TNF-α	6	2 060±112 *
50 mg/L emodin	6	1 723±143^△
100 mg/L emodin	6	1 376±109^▲

Group	n	p27
Control	6	1.06±0.11
TNF-α	6	0.56±0.08
50 mg/L emodin	6	0.71±0.12^△
100 mg/L emodin	6	0.84±0.15^▲

Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-α

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