[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 120-122.doi: 10.3736/jcim20040213

• Original Experimental Research • Previous Articles     Next Articles

Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-α

Xiao-bin Mei, Wei-jie Yuan, Feng-lan Zhan, Hao Wu, Xiao-ying Zhang, Ruo-lan Cui   

  1. Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai, 200433,China
  • Online:2004-03-20 Published:2018-10-25

RichHTML

1

PDF (PC)

125

Like

Objective: To investigate the role of p27 in the inhibition of emodin on the mesangial cell (MC) proliferation induced by tumor necrosis factor-α (TNF-α). Methods: p27 protein of MC was detected with western blotting analysis. The degree of MC proliferation was estimated through[ 3H]thymidine ([ 3H]TdR) incorporation. Different dosage of emodin (50 mg/L,100 mg/L) was added into MC stimulated by TNF-α. Results: TNF-α (200 kU/L) decreased p27 level of MC cultured in serum-free DMEM for 24 hours and increased[ 3H]TdR incorporation. Emodin increased p27 level of MC stimulated by TNF-α and decreased [ 3H]TdR incorporation. The more the emodin was added, the greater the above-mentioned effect of emodin. Conclusion: The increment of p27 level maybe play an important role in the inhibition of emodin on MC proliferation induced by TNF-α.

Key words: emodin, cyclin kinase inhibitor, tumor necrosis factor-α, mesangial cell

CLC Number: 

  • R285.6

Tab 1

Effect of emodin on [3H]TdR incorporation of MC stimulated by TNF-α (ヌ±S)"

Group n [3H]TdR incorporation (cpm/well)
Control 6 685±53
TNF-α 6 2 060±112 *
50 mg/L emodin 6 1 723±143△
100 mg/L emodin 6 1 376±109▲

Figure 1

Effect of emodin on p27 level of MC stimulatedby TNF-α1: Control; 2： TNF-α; 3: 50 mg/L emodin; 4: 100 mg/L emodin"

Tab 2

Effect of emodin on p27 level of MC stimulated by TNF-α (ヌ±S)"

Group n p27
Control 6 1.06±0.11
TNF-α 6 0.56±0.08
50 mg/L emodin 6 0.71±0.12△
100 mg/L emodin 6 0.84±0.15▲
[1] 刘志红, 黎磊石 . 大黄素对肾小球系膜细胞PCNA的影响[J]. 细胞生物学杂志, 1993,15(4):174-176
[2] 戴春笋, 刘志红, 陈惠萍 , 等. 大黄素与雷公藤酯醇联合治疗大鼠抗肾小球基底膜肾炎的试验研究[J]. 肾脏病透析与移植杂志, 2000,9(2):117-123
doi: 10.3969/j.issn.1006-298X.2000.02.004
[3] 刘冠贤, 叶任高, 谭志明 , 等. 大黄素对狼疮性肾炎成纤维细胞生物学行为的影响[J]. 中国中西医结合杂志, 2000,20(3):196-198
[4] 王晓玲, 王俭勤, 夏延龄 , 等. 大黄素对人肾成纤维细胞抑制作用的研究[J]. 中国中西医结合肾病杂志, 2002,3(11):629-631
doi: 10.3969/j.issn.1009-587X.2002.11.004
[5] Shankland SJ . Cell-cycle control and renal disease[J]. Kidney Int, 1997,52(2):294-308
doi: 10.1038/ki.1997.335 pmid: 9263984
[6] 梅小斌, 崔若兰, 高从容 , 等. Thy1肾炎大鼠肾小球周期素激酶抑制剂p27的变化[J]. 中华肾脏病杂志, 2001,17(5):335-336
[7] 梅小斌, 崔若兰, 高从容 , 等. 周期素激酶抑制剂p27在肿瘤坏死因子-α诱导系膜细胞增生中的作用[J]. 中华内科杂志, 2002,41(9):599-601
doi: 10.3760/j.issn:0578-1426.2002.09.008
[8] Shankland SJ, Pippin J, Flanagan M , et al. Mesangial cell proliferation mediated by PDGF and bFGF is determined by levels of the cyclin kinase inhibitor p27Kip1[J]. Kidney Int, 1997,51(4):1088-1099
[9] 谌贻璞, 高进, 邢宝才 , 等. 肾小球系膜细胞培养[J]. 北京医科大学学报, 1989,21(4):335-336
[10] Terada Y, Inoshita S, Nakashima O , et al. Lovastatin inhibits mesangial cell proliferation via p27[J]. J Am Soc Nephrol, 1998,9(12):2235-2243
[11] 林秀珍, 马德禄, 崔荣芬 , 等. 大黄素、番泻甙及大黄多糖对培养大鼠肝细胞内游离钙浓度的影响[J]. 中国中西医结合杂志, 1995,15(7):419-421
doi: 10.7661/CJIM.1995.7.419
[12] Short AD, Bian J, Ghosh TK , et al. Intracellular Ca2+ pool content is linked to control of cell growth[J]. Proc Natl Acad Sci USA, 1993,90(11):4986-4990
[1] Rui-chen Zhang, Bin Liu, Zhen-xiao Sun, Dong-yan Xu. Effects of extract of Polygonum multiflorum on cell cycle arrest and apoptosis of human liver cell line L02. Journal of Chinese Integrative Medicine, 2010, 8(6): 554-561.
[2] Jian-wen Ning, Feng Ji, Dan-dong Luo, Chun-yan Yang, Li-jun Wang. Effects of emodin on cell apoptosis of intestinal mucosa and serum leptin in rats with severe acute pancreatitis. Journal of Chinese Integrative Medicine, 2009, 7(12): 1167-1173.
[3] Ting-fang Chen, Ming Chen, Jian-hua Qin, Ming-yong Wang. Interventional effects of emodin on transforming growth factor-beta1/integrin-linked kinase signal way in interleukin-1β-induced transdifferen-tiation of rat tubular epithelial-myofibroblasts. Journal of Chinese Integrative Medicine, 2009, 7(1): 59-64.
[4] Wan-sen Sun, Zhu Wang, Juan Wang, Qiao-ya Ma, Xiao-qiang Zhang, Xi-li Wu. Effects of Qufeng Tongluo Recipe on proliferation of and TGF-β1 and IL-6 mRNA expressions in glomerular mesangial cells from rats. Journal of Chinese Integrative Medicine, 2008, 6(9): 915-920.
[5] Yong-hong Li, Zong-wen Huang, Ping Xue, Jia Guo, Fu-qian He, Zheng You, Zheng-rong Wang. Effects of Chaiqin Chengqi Decoction on activation of nuclear factor-κB in pancreas of rats with acute necrotizing pancreatitis. Journal of Chinese Integrative Medicine, 2008, 6(2): 180-184.
[6] Hui Yang, Yi Li, Ling Cheng, Jin-sen He. Effect of electroacupuncture and diet adjusting on insulin resistance in rats with nutrition obesity. Journal of Chinese Integrative Medicine, 2007, 5(5): 546-549.
[7] Jing-hua Peng, Zhi-hong Fang, Jian-wei Cui, Qin Feng, Li-li Xu, Hong-tu Gu, Yi-yang Hu. Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide. Journal of Chinese Integrative Medicine, 2007, 5(3): 302-306.
[8] Jing Mu, Yi-kui Li, Sheng-guang Fu. Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats. Journal of Chinese Integrative Medicine, 2003, 1(2): 122-124.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]