SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 180-184.doi: 10.3736/jcim20080215

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chaiqin Chengqi Decoction on activation of nuclear factor-κB in pancreas of rats with acute necrotizing pancreatitis

Yong-hong Li1,2, Zong-wen Huang1(), Ping Xue1, Jia Guo1, Fu-qian He1, Zheng You1, Zheng-rong Wang2   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China
    2. Department of Biomedical Engineering, School of West China Basic Medicine and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China
  • Received:2007-06-13 Online:2008-02-20 Published:2008-02-15
  • Contact: HUANG Zong-wen E-mail:huangzongwen@medmail.com.cn

RichHTML

1

PDF (PC)

103

Like

Objective:To explore the mechanism of Chaiqin Chengqi Decoction (CQCQD) in treatment of rats with acute necrotizing pancreatitis (ANP).

Methods:Thirty SD rats were randomly divided into 3 groups: sham-operated (SO) group, ANP group and CQCQD-treated group. ANP was induced by retro-pumping 3.5% sodium cholate to common bile duct. Blood sample was collected from abdominal vein for examination and the pancreatic tissue samples were taken for making pathology section 6 hours later. The pancreatic tissue (HE staining) was observed by light microscope. The content of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was detected with the method of enzyme-linked immunosorbent assay, and the activation of nuclear factor-κB (NF-κB) in pancreas was detected by immunohistochemical method.

Results:Compared with the SO group, there was dramatic increase in the white blood cell (WBC) counts and AMY level in the ANP group (P<0.05, P<0.01). Compared with the ANP group, the WBC counts and AMY level in CQCQD-treated group were significantly reduced (P<0.05). The edema, inflammatory infiltration, haemorrhage and necrosis scores and total pathological score in the ANP group were obviously higher than those in the SO group (P<0.01). The edema, haemorrhage and inflammatory infiltration scores and the total pathological score in CQCQD-treated group were decreased (P<0.05). The integral optical density of NF-κB p65 positive cells of pancreas in CQCQD-treated group was lower than that in the ANP group (P<0.05).

Conclusion:CQCQD can reduce the content of serum TNF-α and IL-6, depress the activation of NF-κB, and lessen the pancreatic lesions.

Key words: acute necrotizing pancreatitis, nuclear factor kappa B, Chaiqin Chengqi Decoction, tumor necrosis factor α, interleukin-6, rats

CLC Number: 

  • R289.5

Table 1

AMY content and WBC counts in SO, ANP and CQCQD-treated groups ($\bar{x}$±s)"

Group n AMY （U/L） WBC （×109/L）
Sham-operated group 10 748.8±64.1 8.8±2.7
ANP group 10 4 041.4±1 087.7** 12.8±3.8**
CQCQD-treated group 10 1 901.0±204.2**△△ 8.5±1.6**△△

Table 2

Inflammatory factor expression and IOD of NF-κB p65 in SO, ANP and CQCQD-treated groups ($\bar{x}$±s)"

Group n IL-6 (ng/L) TNF-α (ng/L) IOD of NF-κB p65
Sham-operated group 10 86.2±17.2 13.6±2.1 88.0±43.0
ANP group 10 338.5±47.0** 124.0±36.0** 579.1±338.4**
CQCQD-treated group 10 137.5±12.7**△△ 52.9±7.4**△△ 136.9±90.6**△

Figure 1

Expression of NF-κB p65 positive cells in pancreas tested by immunohistochemical method in 3 groups (Light microscope, ×400) A: SO group; B: ANP group; C: CQCQD-treated group."

Table 3

Pancreatic pathological scores in SO, ANP and CQCQD-treated groups ($\bar{x}$±s)"

Group n Edema score Inflammatory
infiltration score		 Haemorrhage
score		 Necrosis
score		 Total pathological
score
Sham-operated group 10 0.4±0.7 0.4±0.5 0 0 0.8±1.0
ANP group 10 3.2±0.6** 2.5±1.0** 2.6±0.7** 2.1±1.0** 10.2±2.7**
CQCQD-treated group 10 2.0±0.7△ 1.4±1.0△ 1.7±0.7△ 1.8±0.9 6.9±1.7△

Figure 2

Pathological section of pancreatic tissue under light microscope (HE staining, ×400) A: SO group; B: ANP group; C: CQCQD-treated group."

[1] de Beaux AC, Ross JA, Maingay JP , et al. Proinflam- matory cytokine release by peripheral blood mononuclear cells from patients with acute pancreatitis. Br J Surg. 1996; 83(3):1071-1075.
[2] Rongione AJ, Kusske AM, Kwan K , et al. Interleukin 10 reduces the severity of acute pancreatitis in rats. Gastroenterology. 1997; 112(3):960-967.
doi: 10.1053/gast.1997.v112.pm9041259 pmid: 9041259
[3] Dambrauskas Z, Pundzius J, Barauskas G . Predicting development of infected necrosis in acute necrotizing pancreatitis. Medicina (Kaunas). 2006; 42(6):441-449.
pmid: 16816537
[4] Andoh A, Hata K, Shimada M , et al. Inhibitory effects of somatostatin on tumor necrosis factor-alpha-induced interleukin-6 secretion in human pancreatic periacinar myofibroblasts. Int J Mol Med. 2002; 10(1):89-93.
[5] Zhang ZD, Yan LN, Liu XB. Acute pancreatitis. Beijing: Peple's Medical Publishing House. 2004: 47-48. Chinese.
张肇达, 严律南, 刘续宝 . 急性胰腺炎.北京: 人民卫生出版社. 2004: 47-48.
[6] Mercurio F, Manning AM . Multiple signals converging on NF-kappaB. Curr Opin Cell Biol. 1999; 11(2) : 226-232.
doi: 10.1016/S0955-0674(99)80030-1
[7] Liu XB, Jiang JM, Huang ZW, et al. Clinical study on the treatment of severe acute pancreatitis by integrated traditional Chinese medicine and Western medicine. Sichuan Da Xue Xue Bao ( Yi Xue Ban ). 2004; 35(2):204-208. Chinese with abstract in English.
刘续宝, 蒋俊明, 黄宗文 , 等. 中西医结合治疗重症急性胰腺炎的临床研究(附1376例报告). 四川大学学报 (医学版). 2004; 35(2):204-208.
[8] Huang ZW, Xia Q, Chen GY , et al. Clinical effect of Chaiqin Chengqi Decoction in treating severe acute pancreatitis in early stage. Chendu Zhong Yi Yao Da Xue Xue Bao. 2003; 26(3):25-26. Chinese.
doi: 10.3969/j.issn.1004-0668.2003.03.010
黄宗文, 夏庆, 陈光远 , 等. 早期应用柴芩承气汤治疗重症急性胰腺炎临床疗效分析.成都中医药大学学报. 2003; 26(3):25-26.
doi: 10.3969/j.issn.1004-0668.2003.03.010
[9] Xue P, Huang ZW, Guo J , et al. Clinical study of Chaiqin Chengqi Decoction in treating severe acute biliary pancreatitis. Zhong Xi Yi Ji He Xue Bao. 2005; 3(4):263-265. Chinese with abstract in English.
doi: 10.7666/d.y793084
薛平, 黄宗文, 郭佳 , 等. 早期应用柴芩承气汤治疗胆源性重症急性胰腺炎的临床研究.中西医结合学报. 2005; 3(4):263-265.
doi: 10.7666/d.y793084
[1] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3