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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (2): 180-184.doi: 10.3736/jcim20080215

• Original Experimental Research • Previous Articles     Next Articles

Effects of Chaiqin Chengqi Decoction on activation of nuclear factor-κB in pancreas of rats with acute necrotizing pancreatitis

Yong-hong Li1,2, Zong-wen Huang1(), Ping Xue1, Jia Guo1, Fu-qian He1, Zheng You1, Zheng-rong Wang2   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, China
    2. Department of Biomedical Engineering, School of West China Basic Medicine and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China
  • Received:2007-06-13 Online:2008-02-20 Published:2008-02-15
  • Contact: HUANG Zong-wen E-mail:huangzongwen@medmail.com.cn

Objective:To explore the mechanism of Chaiqin Chengqi Decoction (CQCQD) in treatment of rats with acute necrotizing pancreatitis (ANP).

Methods:Thirty SD rats were randomly divided into 3 groups: sham-operated (SO) group, ANP group and CQCQD-treated group. ANP was induced by retro-pumping 3.5% sodium cholate to common bile duct. Blood sample was collected from abdominal vein for examination and the pancreatic tissue samples were taken for making pathology section 6 hours later. The pancreatic tissue (HE staining) was observed by light microscope. The content of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was detected with the method of enzyme-linked immunosorbent assay, and the activation of nuclear factor-κB (NF-κB) in pancreas was detected by immunohistochemical method.

Results:Compared with the SO group, there was dramatic increase in the white blood cell (WBC) counts and AMY level in the ANP group (P<0.05, P<0.01). Compared with the ANP group, the WBC counts and AMY level in CQCQD-treated group were significantly reduced (P<0.05). The edema, inflammatory infiltration, haemorrhage and necrosis scores and total pathological score in the ANP group were obviously higher than those in the SO group (P<0.01). The edema, haemorrhage and inflammatory infiltration scores and the total pathological score in CQCQD-treated group were decreased (P<0.05). The integral optical density of NF-κB p65 positive cells of pancreas in CQCQD-treated group was lower than that in the ANP group (P<0.05).

Conclusion:CQCQD can reduce the content of serum TNF-α and IL-6, depress the activation of NF-κB, and lessen the pancreatic lesions.

Key words: acute necrotizing pancreatitis, nuclear factor kappa B, Chaiqin Chengqi Decoction, tumor necrosis factor α, interleukin-6, rats

CLC Number: 

  • R289.5

Table 1

AMY content and WBC counts in SO, ANP and CQCQD-treated groups ($\bar{x}$±s)"

Group n AMY (U/L) WBC (×109/L)
Sham-operated group 10 748.8±64.1 8.8±2.7
ANP group 10 4 041.4±1 087.7** 12.8±3.8**
CQCQD-treated group 10 1 901.0±204.2**△△ 8.5±1.6**△△

Table 2

Inflammatory factor expression and IOD of NF-κB p65 in SO, ANP and CQCQD-treated groups ($\bar{x}$±s)"

Group n IL-6 (ng/L) TNF-α (ng/L) IOD of NF-κB p65
Sham-operated group 10 86.2±17.2 13.6±2.1 88.0±43.0
ANP group 10 338.5±47.0** 124.0±36.0** 579.1±338.4**
CQCQD-treated group 10 137.5±12.7**△△ 52.9±7.4**△△ 136.9±90.6**

Figure 1

Expression of NF-κB p65 positive cells in pancreas tested by immunohistochemical method in 3 groups (Light microscope, ×400) A: SO group; B: ANP group; C: CQCQD-treated group."

Table 3

Pancreatic pathological scores in SO, ANP and CQCQD-treated groups ($\bar{x}$±s)"

Group n Edema score Inflammatory
infiltration score
Haemorrhage
score
Necrosis
score
Total pathological
score
Sham-operated group 10 0.4±0.7 0.4±0.5 0 0 0.8±1.0
ANP group 10 3.2±0.6** 2.5±1.0** 2.6±0.7** 2.1±1.0** 10.2±2.7**
CQCQD-treated group 10 2.0±0.7 1.4±1.0 1.7±0.7 1.8±0.9 6.9±1.7

Figure 2

Pathological section of pancreatic tissue under light microscope (HE staining, ×400) A: SO group; B: ANP group; C: CQCQD-treated group."

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