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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (3): 302-306.doi: 10.3736/jcim20070314

• Original Experimental Research • Previous Articles     Next Articles

Effects of Jianpi Huoxue Decoction on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide

Jing-hua Peng, Zhi-hong Fang, Jian-wei Cui, Qin Feng, Li-li Xu, Hong-tu Gu, Yi-yang Hu   

  1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2007-05-31 Published:2007-05-15

Objective: To observe the effects of Jianpi Huoxue Decoction, a compound Chinese herbal medicine, on Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and lipopolysaccharide (LPS).Methods: SD rats were divided into normal, control liquid diet, ethanol liquid diet and ethanol liquid diet plus Jianpi Huoxue Decoction group. Rats were administrated with Jianpi Huoxue Decoction or distilled water via gastrogavage for 4 weeks after administration with ethanol or control liquid diet for 2 weeks respectively. After that, rats in each group were stimulated with LPS via gastrogavage for 3.5 h and harvested. Alanine aminotransferase (ALT) in serum and triglyceride (TG) in liver were analyzed. Pathological changes in liver tissues were observed in HE staining section. Tumor necrosis factor-α(TNF-α) in portal vein plasma was assayed by ELISA. The protein expressions of CD68, Toll-like receptor 4 (TLR4), phosphorylation-IκB (P-IκB) and TNF-α in liver were evaluated with Western-blotting.Result: After the treatment with Jianpi Huoxue Decoction, the pathologic changes in liver tissue were lightened, levels of ALT in serum, TG in liver and TNF-α in portal vein plasma were decreased, and the protein expressions of CD68, TLR4, P-IκB and TNF-α in liver were reduced.Conclusion: Jianpi Huoxue Decoction can inhibit Kupffer cell signal pathway activation in rats with liver injury induced by Lieber-Decarli liquid diet and LPS.

Key words: liver disease, alcoholic, endotoxin, lipopolysaccharides, tumor necrosis factor-α

CLC Number: 

  • R575

Figure 1

HE staining for liver tissue and immunohistochemical staining for CD68 (×200)A: HE staining for liver tissue; A1: Normal group; A2: Control liquid diet group; A3: Ethanol liquid diet group; A4: Jianpi Huoxue Decoction group. B: Immunohistochemical staining for CD68; B1: Normal group; B2: Control liquid diet group; B3: Ethanol liquid diet group; B4: Jianpi Huoxue Decoction group."

Table 1

Contents of TG in liver, TNF-α in portal vein plasma and activity of ALT in serum (ヌ±S)"

Group n TG in liver (mg/g) TNF-α in portal vein plasma (ng/L) ALT in serum (U/L)
Normal 6 38.93±16.51* 14.97±1.52** 39±4**
Control liquid diet 11 75.37±23.30* 15.91±1.19** 24±7**
Ethanol liquid diet 12 365.59±50.42 29.55±3.37 96±22
Jianpi Huoxue Decoction 12 245.59±63.85*△△ 26.00±2.77*△△ 75±18**

Figure 2

Effects of Jianpi Huoxue Decoction on protein expressions of CD68, TLR4, P-IκB and TNF-α in rats with liver injury induced by Lieber-Decarli diet and LPS (Western-blotting)1: Normal group; 2: Control liquid diet group; 3: Ethanol liquid diet group; 4: Jianpi Huoxue Decoction group; *P<0.05, *P<0.01, vs ethanol liquid diet group."

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