参芪方血清对慢性肾衰大鼠肾脏细胞增殖的影响

doi:10.3736/jcim20030211

Abstract

Objective

To observe the effect of Shenqi Recipe on the proliferation of glomerular mesangial cells (MC) and interstitial fibroblasts (FC) in rats.

Methods

The effect of serum containing prepared drugs from normal and chronic renal failure rats treated by Shenqi Recipe on glomerular mesangial cells and intersititial fibroblasts in vitro was observed by MTT colorimetric assay and serophrmacology.

Results

Compared with the blank sample normal rats, serum containing Shenqi Recipe inhibited the proliferation of MC and FC (P<0.001), and the serum containing Shenqi Recipe of CRF rats had significantly inhibited the proliferation of MC and FC compared with the model group (P<0.001).

Conclusion

Shenqi Recipe can postpone CRF progress by this mechanism.

Key words: Shenqi Recipe, glomerular mesangial cells, interstitial fibroblasts

CLC Number:

R285.5

Jing Mu, Yi-kui Li, Sheng-guang Fu. Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats. Journal of Chinese Integrative Medicine, 2003, 1(2): 122-124.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20030211

http://www.jcimjournal.com/EN/Y2003/V1/I2/122

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血清添加浓度	胎牛血清（OD值）	空白鼠血清（OD值）	含药血清（OD值）
20％	0.226±0.01	0.214±0.01	0.172±0.01**
40％		0.294±0.03	0.172±0.04**

血清添加浓度	胎牛血清（OD值）	空白鼠血清（OD值）	含药血清（OD值）
10％	1.41±0.05	1.47±0.08	1.05±0.12***
20％	–	1.44±0.06	1.23±0.02***
40％	–	1.35±0.08	0.79±0.12***

组别	添加浓度	OD值
假手术组	20％	1.03±0.06**
模型组	20％	1.28±0.25
参芪组	20％	0.81±0.24**
洛汀新组	20％	0.82±0.07**

组别	添加浓度	OD值
假手术组	10％	1.40±0.06*
模型组	10％	1.48±0.08
参芪组	10％	1.18±0.14**
洛汀新组	10％	1.26±0.11**

Influence of Shenqi Recipe on mesangial cells and interstitial fibroblasts of chronic renal failure rats

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