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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (2): 120-122.doi: 10.3736/jcim20040213

• Original Experimental Research • Previous Articles     Next Articles

Role of cyclin kinase inhibitor p27 in inhibition of emodin on mesangial cell proliferation induced by tumor necrosis factor-α

Xiao-bin Mei, Wei-jie Yuan, Feng-lan Zhan, Hao Wu, Xiao-ying Zhang, Ruo-lan Cui   

  1. Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai, 200433,China
  • Online:2004-03-20 Published:2018-10-25

Objective: To investigate the role of p27 in the inhibition of emodin on the mesangial cell (MC) proliferation induced by tumor necrosis factor-α (TNF-α). Methods: p27 protein of MC was detected with western blotting analysis. The degree of MC proliferation was estimated through[ 3H]thymidine ([ 3H]TdR) incorporation. Different dosage of emodin (50 mg/L,100 mg/L) was added into MC stimulated by TNF-α. Results: TNF-α (200 kU/L) decreased p27 level of MC cultured in serum-free DMEM for 24 hours and increased[ 3H]TdR incorporation. Emodin increased p27 level of MC stimulated by TNF-α and decreased [ 3H]TdR incorporation. The more the emodin was added, the greater the above-mentioned effect of emodin. Conclusion: The increment of p27 level maybe play an important role in the inhibition of emodin on MC proliferation induced by TNF-α.

Key words: emodin, cyclin kinase inhibitor, tumor necrosis factor-α, mesangial cell

CLC Number: 

  • R285.6

Tab 1

Effect of emodin on [3H]TdR incorporation of MC stimulated by TNF-α (ヌ±S)"

Group n [3H]TdR incorporation (cpm/well)
Control 6 685±53
TNF-α 6 2 060±112 *
50 mg/L emodin 6 1 723±143
100 mg/L emodin 6 1 376±109

Figure 1

Effect of emodin on p27 level of MC stimulatedby TNF-α1: Control; 2: TNF-α; 3: 50 mg/L emodin; 4: 100 mg/L emodin"

Tab 2

Effect of emodin on p27 level of MC stimulated by TNF-α (ヌ±S)"

Group n p27
Control 6 1.06±0.11
TNF-α 6 0.56±0.08
50 mg/L emodin 6 0.71±0.12
100 mg/L emodin 6 0.84±0.15
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