祛风通络方对大鼠系膜细胞增殖及转化生长因子β1和白细胞介素6 mRNA表达的影响

doi:10.3736/jcim20080908

Abstract

Objective: To investigate the effects of Qufeng Tongluo Recipe (QFTLR), a compound of traditional Chinese herbal medicine for dispelling wind and removing obstruction in the meridians, on cell proliferation and expressions of transforming growth factor-beta1 (TGF-β1) and interleukin-6 (IL-6) mRNAs induced by lippolysaccharide in glomerular mesangial cells from rats.
Methods: The method of serum pharmacology was used. A total of 32 rats were divided into normal control group, untreated group, QFTLR group and positive control group which also was named Monopril (fosinopril sodium) group. Mesangial proliferative glomerulonephritis was induced by injection of antithymocyte serum in the rats except for the normal control group. Sera of the rats were obtained after corresponding interventions. Lipopolysaccharide-induced proliferation of rat mesangial cells (MCs) cultured in the respective serum-containing media was detected by the method of methyl thiazolyl tetrazolium (MTT) assay, and the expressions of TGF-β1 and IL-6 mRNAs were analyzed by the method of reverse transcription polymerase chain reaction.
Results: Compared with the untreated group, QFTLR showed remarkable inhibitory function on the proliferation of the mesangial cells (P<0.05). The expressions of TGF-β1 mRNA in mesangial cells were increased in the untreated group, QFTLR group and Monopril group when compared with the normal control group (P<0.01), but the TGF-β1 mRNA expressions in QFTLR group and in Monopril group were lower than that in the untreated group. The IL-6 mRNA expression could be increased by the LPS stimulation, and it was significantly higher in the other three groups than that in the normal control group, including the untreated group, the Monopril group and the QFTLR group (P<0.01). Compared with the untreated group, the expression of IL-6 mRNA was decreased by QFTLR and Monopril (P<0.01). QFTLR was better than Monopril in inhibiting the proliferation of the mesangial cells and decreasing the expressions of TGF-β1 and IL-6 mRNAs (P<0.05).
Conclusion: QFTLR has great inhibitory effect on mesangial cell proliferation and expressions of TGF-β1 and IL-6 mRNAs, which may be one of its mechanisms in postponing glomerular sclerosis.

Key words: Qufeng Tongluo Recipe, mesangial cell, pharmacology (TCD), transforming growth factor-betal, interleukin-6, rats

CLC Number:

R295.5

Wan-sen Sun, Zhu Wang, Juan Wang, Qiao-ya Ma, Xiao-qiang Zhang, Xi-li Wu. Effects of Qufeng Tongluo Recipe on proliferation of and TGF-β1 and IL-6 mRNA expressions in glomerular mesangial cells from rats. Journal of Chinese Integrative Medicine, 2008, 6(9): 915-920.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20080908

http://www.jcimjournal.com/EN/Y2008/V6/I9/915

TrendMD

Figures/Tables 5

Table 1

Figure 1

Table 2

Figure 2

Figure 3

References 8

[1]	Kashgarian M, Sterzel RB . The pathobiology of the mesangium[J]. Kidney Int, 1992,41(3):524-529 doi: 10.1038/ki.1992.74 pmid: 1573822
[2]	Dong B, Zou WZ, You JF . A study on the proliferative and sclerosing mechanism of glomerular mesangium[J]. Zhonghua Bing Li Xue Za Zhi, 1994,23(1):10-13 doi: 10.1007/BF02007173
	董葆, 邹万忠, 由江峰 . 肾小球系膜细胞增生与系膜硬化机制的初步探讨[J]. 中华病理学杂志, 1994,23(1):10-13 doi: 10.1007/BF02007173
[3]	Liu JF, Wen JK, Yan X , et al.Model of rat with mesangial proliferative glomerulonephritis induced by antithymocyte serum[J].Zhongguo Bing Li Sheng Li Za Zhi, 1999, 15(3): 274, 277
	刘静方, 温进坤, 严霞 , 等 .抗胸腺细胞血清法诱发大鼠系膜增殖性肾炎模型[J].中国病理生理杂志, 1999, 15(3): 274, 277
[4]	Sun L, Yi ZW, Yu PL . Effect of tetramethylhyrazine on proliferation of human fetal mesangial cells[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1995,15(3):134-136 doi: 10.7661/CJIM.1995.3.134
	孙林, 易著文, 虞佩兰 . 川芎嗪对人胎肾小球系膜细胞增殖的影响及其机理探讨[J]. 中国中西医结合杂志, 1995,15(3):134-136 doi: 10.7661/CJIM.1995.3.134
[5]	Fu P, Liu F, Su S , et al. Signaling mechanism of renal fibrosis in unilateral ureteral obstructive kidney disease in ROCK1 knockout mice[J]. J Am Soc Nephrol, 2006,17(11):3105-3114 doi: 10.1681/ASN.2005121366
[6]	Lu HZ, Zhou JH . Role of transforming growth factor-β on signal transduction and mesangial cell in glomerular fibrosis[J]. Shi Yong Er Ke Lin Chuang Za Zhi, 2007,22(17):1346-1348
	卢宏柱, 周建华 . 转化生长因子-β作用于系膜细胞的信号转导与肾小球纤维化[J]. 实用儿科临床杂志, 2007,22(17):1346-1348
[7]	Zhang J, Yu YH, Luo LX , et al. Effect of Yushen Granule on expression of TGF-β in mesangial cells in rats with angiotensin Ⅱ irritating[J]. Zhongguo Zhong Xi Yi Jie He Shen Bing Za Zhi, 2005,6(6):354-356
	张君, 于英华, 罗立欣 , 等. 愈肾颗粒对血管紧张素Ⅱ刺激大鼠肾系膜细胞TGF-β表达的影响[J]. 中国中西医结合肾病杂志, 2005,6(6):354-356
[8]	Ruef C, Budde K, Lacy J , et al. Interleukin 6 is an autocrine growth factor for mesangial cells[J]. Kidney Int, 1990,38(2):249-257 doi: 10.1038/ki.1990.193 pmid: 2402117222

Related Articles 15

[1]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2]	Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3]	Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4]	Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5]	Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6]	Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7]	Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8]	Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9]	Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10]	Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11]	Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12]	Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13]	Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14]	Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng . Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15]	Amir Rashidian, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2]	Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3]	Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4]	SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5]	Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6]	Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8]	Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9]	Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10]	Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239

Group	n	OD
Normal control	6	1.253±0.149
Untreated	6	1.960±0.205*
Monopril	6	1.712±0.121*
QFTLR	6	1.386±0.111^△▲

Group	n	TGF-β1 mRNA/β-actin	IL-6 mRNA/β-actin
Normal control	3	0.267±0.009	0.350±0.012
Untreated	3	0.740±0.012**	0.940±0.012**
Monopril	3	0.663±0.009**^△△	0.853±0.009**^△△
QFTLR	3	0.443±0.062**^△△▲▲	0.627±0.018**^△△▲▲

Effects of Qufeng Tongluo Recipe on proliferation of and TGF-β1 and IL-6 mRNA expressions in glomerular mesangial cells from rats

RichHTML

PDF (PC)

Like