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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (3): 193-195.doi: 10.3736/jcim20040312

• Original Experimental Research • Previous Articles     Next Articles

Effects of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes

Shu-hai Wang1(), Wen-jian Wang1, Xue-feng Wang2, Wei-hua Chen1   

  1. 1. Institute of Integrated Traditional Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
    2. Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Online:2004-06-20 Published:2018-10-25

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Objective: To observe the effects of salidroside on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes. Methods: 3H-glucose uptaking rate in adipocytes treated by salidroside was detected. Adipocytes treated by salidroside in differentiation were stained by oil red O and analyzed by spectrophotography quantitatively. The expression of PPAR-γ and C/EBP-α mRNA relating to differentiation of adipocytes was detected by reverse transcription PCR. Results: 3H-glucose uptaking rate in the salidroside group was 110.4％, higher than that in the blank control group (P<0.01). Salidroside suppressed the differentiation of 3T3-L1 pre-adipocytes and down-regulated the expression of PPAR-γ and C/EBP-α mRNA (P<0.01, vs control group). Conclusion: Salidroside promotes the 3H-glucose uptaking, suppresses the differentiation and down-regulates the expression of PPAR-γ and C/EBP-α mRNA in 3T3-L1 adipocytes.

Key words: salidroside, 3T3 cells, cells differentiation, metabolism, glucose

CLC Number: 

  • R285

Figure 1

3H-glucose uptaking rate (n=7)**P<0.01, vs blank control group; △△ P<0.01,vs rosiglitazone treated group"

Tab 1

Differentiation of 3T3-L1 pre-adipocytes (ヌ±S)"

Group n OD
Salidroside treated 12 0.404±0.022**△△
Rosiglitazone treated 12 0.700±0.033**
Blank control 12 0.538±0.053△△

Figure 3

Agarose gel electrophoresis of PPAR-γ1. Salidroside treated; 2. Rosiglitazone treated; 3. Blank control"

Figure 3

Expression of PPAR-γmRNA in 3T3-L1 adipocytes ( n = 6)* * P＜ 0. 01, vs blank control group; △ △ P＜ 0. 01,vs rosiglitazone treated group"

Figure 4

Agarose gel electrophoresis of C/EBP-α1. Salidroside treated; 2. Rosiglitazone treated; 3. Blank control"

Figure 5

Expression of C/EBP-α mRNA in 3T3-L1 adipocytes(n=6)**P<0.01, vs blank control group; △△P<0.01,vs rosiglitazone treated group"

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